Int J Stomatol

Int J Stomatol ›› 2024, Vol. 51 ›› Issue (3): 288-295.doi: 10.7518/gjkq.2024044

• Original Articles • Previous Articles     Next Articles

Correlation study of plasma cell-free extra-mitochondrial mitochondria DNA and periodontitis clinical parameters

Zaimu Yang1(),Pei Cao1,Zhenhua Liu2,Qingxian Luan1()   

  1. 1.Dept. of Periodontology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
    2.Dept. of Stomatology, Beijing Zhongguancun Hospital, Beijing 100190, China
  • Received:2023-08-16 Revised:2024-02-01 Online:2024-05-01 Published:2024-05-06
  • Contact: Qingxian Luan E-mail:yangzaimu@pku.org.cn;kqluanqx@126.com

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Abstract:

Objective Plasma cell-free extra-cellular mitochondrial DNA (cf-exmtDNA) shows pro-inflammatory potential. This study aims to investigate the correlation between cf-exmtDNA and baseline periodontitis clinical parameters in patients in general health. Methods A total of 78 participants aged 18-45 years were enrolled: 11 periodontal healthy volunteers, 11 patients with gingivitis, and 56 patients with periodontitis. Baseline periodontal index, age, gender, body mass index (BMI), and baseline fasting blood glucose (FBG) were examined and recorded. Anticoagulated venous blood (4 mL) was collected, and cf-mtDNA was extracted by secondary centrifugation. cf-exmtDNA concentration was measured through real-time quantitative polymerase chain reaction. Different states of periodontal inflammation were compared in terms of plasma cf-exmtDNA copy number, and the relationship among plasma cf-exmtDNA, mean probing depth (mPD), mean clinical attachment level (mCAL), mean bleeding index (mBI), mean plaque index, age, FBG, and BMI were examined through correlation and multiple linear regression analyses. Results Patients with periodontitis had significantly higher plasma cf-exmtDNA levels than the healthy volunteers (P=0.042). The overall plasma cf-exmtDNA was significantly positively correlated with age (P=0.023), mPD (P<0.001), mCAL (P=0.006), and mBI (P=0.026). Multiple regression analysis showed that plasma cf-exmtDNA level was significantly dependent on mPD. Conclusion In the general population aged 18–45, patients with periodontitis had significantly higher plasma cf-exmt-DNA level than the healthy volunteers, and plasma cf-exmtDNA concentration was significantly positively correlated with age, mPD, mCAL, and mBI.

Key words: periodontitis, cell-free DNA, mitochondrial DNA, cross-sectional study, periodontal clinical parameters

CLC Number: 

  • R781.4

TrendMD: 

Tab 1

qPCR program setting"

程序温度/℃时长
保持期502 min
预变性9510 min
PCR期（40 个循环）变性9510 s
退火6015 s
延伸a7230 s
熔解曲线期

Tab 2

Primer sequences"

基因方向序列
ND1正向5’-CACACTAGCAGAGACCAACCGAAC-3’
反向5’-CGGCTATGAAGAATAGGGCGAAGG-3’

Tab 3

Baseline characteristics of periodontal healthy, gingivitis, periodontitis participants n=78"

纳入指标组别
牙周健康组（n=11）牙周炎组（n=56）
男/女4/78/330/26
年龄/岁25（23，29）26（24，30）32（26，35）
空腹血糖/（mmol/L）4.80±0.295.05±0.064.96±0.17
体质指数/（kg/m2）20.89±4.7422.22±6.8221.27±4.73
mPD/mm2.16（2.11，2.39）2.35（2.10，2.40）2.97（2.73，3.67）
mCAL/mm0.03（0.00，0.03）0.03（0.00，0.05）3.07（2.77，3.80）
mBI1.32±0.191.66±0.292.53±0.34
mPLI1.15±0.241.40±0.621.55±0.25

Fig 1

The relationship between plasma cf-exmtDNA and periodontal inflammation status and clinical indicators"

Tab 4

Correlations between clinical parameters and plasma cf-exmtDNA"

测量指标与血浆cf-exmtDNA的相关系数
r值P值
牙周指标mPD/mm0.409<0.001
mCAL/mm0.3100.006
mBI0.2520.026
mPLI0.0090.936
全身指标年龄/岁0.2570.023
BMI /（kg/m2）0.2060.071
FBG/（mmol/L）-0.0290.802

Tab 5

Multiple linear regression of plasma cf-exmtDNA and periodontal clinical parameters"

多重线性回归模型模型参数B95% CIβt值P值
血浆cf-exmtDNA常量-1 257.091（-2 090.559，-423.622）-3.0040.004
mPD528.636（258.892，798.380）0.4093.903<0.001
R2adj=0.156（n=78，P<0.001）
mPD常量1.117（0.774，1.459）6.491<0.001
血浆cf-exmtDNA1.73×10-4（0.68×10-4，2.77×10-4）0.2233.2850.002
mBI0.811（0.662，0.960）0.73710.841<0.001
R2adj=0.667（n=78，P<0.001）
mBI常量-0.223（-0.626，0.180）-1.1030.273
mPD0.672（0.551，0.793）0.73911.056<0.001
mPLI0.310（0.136，0.484）0.2373.5530.001
R2adj=0.674（n=78，P<0.001）

Fig 2

Multiple variables bubble plot (left) and multiple linear regression model (right) of mPD"

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