Int J Stomatol

Inter J Stomatol ›› 2019, Vol. 46 ›› Issue (1): 5-11.doi: 10.7518/gjkq.2019.01.002

• Original Articles • Previous Articles     Next Articles

Expression of periostin and p53 in oral leukoplakia and oral squamous cell carcinoma

Qun Li,Weiqun Guan(),Yang’an Zhang,Zhichao. Huang   

  1. Dept. of Stomatology, Fujian Medical University Union Hospital, Fuzhou 350001, China
  • Received:2018-05-15 Revised:2018-10-10 Online:2019-01-01 Published:2019-01-11
  • Contact: Weiqun Guan E-mail:gwqluck@126.com
  • Supported by:
    This study was supported by Fujian Foundation for Medical Innovation(2018CXB-9)

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Abstract:

Objective This study aimed to investigate the expression and clinical significance of periostin and p53 in oral leukoplakia and oral squamous cell carcinoma. Methods The material for immunohistochemical studies consisted of 30 cases of normal oral mucosa, 30 cases of hyperplasia leukoplakia, 38 cases of dysplastic leukoplakia and 79 cases of oral squamous cell carcinoma. Results The index of positive cell vessels and the value of integrated optical density of periostin and p53 between every two groups, except for oral normal mucosa and hyperplasia leukoplakia, were statistically significant (P<0.05). Periostin expression in dysplastic leukoplakia and oral squamous cell carcinoma positively correlated with that of p53. Furthermore, the expression levels of periostin and p53 were significantly related to TNM stage, tumour differentiation degree and lymph node metastasis (P<0.05). Conclusion Periostin and p53 may be involved in the carcinogenesis of oral squamous cell carcinoma and associated with tumour invasion.

Key words: periostin, p53, oral leukoplakia, oral squamous cell carcinoma, carcinogenesis

CLC Number: 

  • R7303.231

TrendMD: 

Tab 1

The index of positive cell vessels and the IA value of periostin in oral normal mucosa, oral leukoplakia and squamous cell carcinoma"

组织类别 例数 阳性表达例数 阳性细胞面积/μm2 IA值（×10-6）
正常黏膜 30 8 2.51±1.30 15.41±1.63
单纯增生白斑 30 10 2.70±1.26 15.66±1.17
异常增生白斑 38 26
轻度异常 17 11 8.70±1.60 26.43±1.50
中/重度异常 21 15 14.23±0.42 33.13±1.07
鳞状细胞癌 79 77
Ⅰ期 21 20 18.69±2.81 38.15±1.42
Ⅱ期 19 18 26.77±2.03 44.26±2.11
Ⅲ期 19 19 33.47±1.77 51.29±2.67
Ⅳ期 20 20 45.36±1.56 60.01±1.36

Fig 1

Periostin and p53 expression in oral normal mucosa, hyperplasia leukoplakia and moderate dysplasis Elivision staining × 400"

Fig 2

Periostin and p53 expression in oral squamous cell carcinomas Elivision staining × 400"

Tab 2

The index of positive cell vessels and the IA value of p53 in oral normal mucosa, oral leukoplakia and squamous cell carcinoma"

组织类别 例数 阳性表达例数 阳性细胞面积/μm2 IA值（×10-6）
正常黏膜 30 0 0 0
单纯增生白斑 30 4 4.68±0.12 5.73±0.94
异常增生白斑 38 20
轻度异常 17 5 11.40±0.89 21.65±1.52
中/重度异常 21 15 12.50±0.24 28.35±0.64
鳞状细胞癌 79 62
Ⅰ期 21 14 17.10±1.45 33.37±1.25
Ⅱ期 19 16 24.33±1.89 39.48±2.01
Ⅲ期 19 15 30.05±2.05 46.51±1.92
Ⅳ期 20 17 37.16±1.75 56.31±1.78

Tab 3

The positive expression of periostin and p53 in oral normalmucosa, oral leukoplakia and squamous cell carcinoma"

骨膜蛋白 p53
组织类别 例数 阳性细胞数 χ2 P 阳性细胞数 χ2 P
（阳性率） （阳性率）
正常黏膜 30 8（26.7%） 0（0.0%）
单纯增生白斑 30 10（33.3%） 1.22* 0.26 4（13.3%） 6.35** 0.01
异常增生白斑 38 26（68.4%） 1.32* 0.21 20（52.6%） 17.93** 0.00
鳞状细胞癌 79 77（97.4%） 9.07** 0.00 62（78.4%） 27.51** 0.00

Tab 4

Relationship between periostin, p53 expressions and clinical pathological parameters in oral squamous cell carcinomas"

骨膜蛋白 p53
临床病理因素 例数 阳性 阴性 χ2 P 阳性 阴性 χ2 P
例数 例数 例数 例数
性别 男 42 33 9 1.353 0.245 31 11 1.149 0.257
女 37 31 6 29 8
年龄 >60 55 54 1 0.574 0.449 48 7 0.690 0.313
≤60 24 22 2 23 1
吸烟 是 43 37 6 0.618 0.137 30 13 0.759 0.416
否 36 29 7 27 9
分化程度 高 34 29 5 4.800 0.028 26 8 20.230 0.000
中/低 45 41 4 34 11
TNM分期 Ⅰ~Ⅱ 38 29 9 5.593 0.018 37 1 6.580 0.015
Ⅲ~Ⅳ 41 33 8 35 6
淋巴结转移 是 48 39 9 5.792 0.016 36 12 5.390 0.021
否 31 27 4 28 3
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