Int J Stomatol

Int J Stomatol ›› 2020, Vol. 47 ›› Issue (2): 159-165.doi: 10.7518/gjkq.2020039

• Original Articles • Previous Articles     Next Articles

Expression and potential clinical significance of Transformer 2β in oral squamous cell carcinoma

Hao Fu1,Ning Yi1,Sun Rui2(),Zheng Xiaoxu3   

  1. 1. Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, China
    2. Dept. of Oral and Maxillofacial Surgery, Shanxi Provincial People’s Hospital, Taiyuan 030012, China
    3. Dept. of Nephrology, Affiliated Hospital of Medical College, University of Maryland, Baltimore 21201, USA
  • Received:2019-07-05 Revised:2019-12-10 Online:2020-03-01 Published:2020-03-12
  • Contact: Rui Sun E-mail:ruidoctor@163.com
  • Supported by:
    This study was supported by Program for Natural Science Foundation of Shanxi Province(2011011037-2)

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Abstract:

Objective This study aimed to investigate the expression and clinical significance of Transformer 2β (Tra2β) in oral squamous cell carcinoma (OSCC). Methods Sixty-four samples of OSCC were subjected to immunohistochemistry to analyze the expression of Tra2β protein in OSCC and to explore the relationship between the expression of Tra2β protein and different clinical pathological parameters. Twenty-two pairs of fresh OSCC tissues and adjacent tissues were used to explore the expression of Tra2β mRNA and protein through real-time polymerase chain reaction and Western blot analyses. The Kaplan-Meier method was used to estimate and validate the effect of Tra2β protein on the survival rate of patients with OSCC. Cox multivariate regression was used to analyze the effect of Tra2β protein on the prognosis of patients with OSCC. Results Immunohistochemistry results revealed that Tra2β protein was expressed in OSCC, and the positive expression of Tra2β protein was significantly associated with the differentiation degree and clinical stage of OSCC. Moreover, the expression trends of Tra2β protein and mRNA were consistent, and the expression levels of Tra2β protein and mRNA in OSCC tissues were higher than those in adjacent tissues. Kaplan-Meier survival analysis showed that patients with OSCC and Tra2β protein expression had poor prognoses. Cox multivariate regression analysis demonstrated that the Tra2β protein was an independent prognostic factor of OSCC. Conclusion Tra2β may be a new molecular target for the treatment of OSCC.

Key words: Transformer 2β, oral squamous cell carcinoma, prognosis

CLC Number: 

  • R739.81

TrendMD: 

Tab 1

Sequence of RT-PCR primers and length of products"

基因 引物（5’~3’） 产物长度/bp
Tra2β 正向引物：TGCGGAGGCGGTGCGGAGCATT;反向引物：CATGACTCCTGGCTGCTGTCGCC 149
GADPH 正向引物：CAACAGCCTCAAGATCATCAGC;反向引物：CATGAGTCCTTCCACGATACCA 103
临床病理参数 阴性（-） 弱阳性（+） 中阳性（++） 强阳性（+++） P值
年龄/岁 >60 3 18 13 2 0.341
≤60 0 15 9 4
性别 男 1 16 10 5 0.443
女 2 17 12 1
淋巴结转移 是 0 3 7 1 0.162
否 3 30 15 5
病变部位 唇 1 10 5 0 0.235
舌 0 2 3 1
颊 0 3 4 1
牙龈 1 17 7 2
腭 1 1 3 2
临床分期 T1 1 18 5 2 <0.001*
T2 1 6 6 2
T3 1 6 6 0
T4 0 3 5 2
分化程度 高分化 1 26 3 1 0.046*
中分化 1 6 18 3
低分化 1 1 1 2

Fig 1

Immunohistochemical staining of Tra2β protein expression in OSCC"

Tab 2

Relationship between the expression of Tra2β protein and clinical pathological data of 64 OSCC"

Fig 2

The expression of Tra2β protein in tumor and paracancerous tissues in OSCC"

Fig 3

The relative expression of Tra2β mRNA in tumors and paracan-cerous tissues of 22 OSCC"

Fig 4

Effect of Tra2β protein expression on survival rate of OSCC patients"

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