Int J Stomatol

Int J Stomatol ›› 2021, Vol. 48 ›› Issue (1): 50-57.doi: 10.7518/gjkq.2021013

• Original Articles • Previous Articles     Next Articles

Expression and clinical significance of lipopolysaccharide binding protein in oral squamous cell carcinoma

Huang Junwen1,Qiao Jie2,Mei Zi1,Chen Zhuo1,Li Yang1,Qiao Bin1()   

  1. 1. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
    2. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
  • Received:2020-05-26 Revised:2020-09-16 Online:2021-01-01 Published:2021-01-20
  • Contact: Bin Qiao E-mail:qiaobin@zzu.edu.cn
  • Supported by:
    This study was supported by Project of Department of Science and Technology of Henan Province(182300410319)

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Abstract:

Objective To investigate the expression and clinical importance of lipopolysaccharide binding protein(LBP) in oral squamous cell carcinoma (OSCC). Methods LBP expression in 48 OSCC tissues and matched adjacent normal tissues and LBP serum levels in 130 patients with OSCC and 90 normal individuals were measured by immunohistochemistry staining assay and enzyme-linked immunosorbent assay, respectively. The relationship between LBP expression and the clinicopathologic characteristics of OSCC was analysed by statistical methods. The diagnostic value of LBP serum level in OSCC was explored via receiver operating characteristic (ROC) curve. Results LBP expression was significantly upregulated in OSCC tissues, whereas little was expressed in adjacent normal tissues (P<0.001). Furthermore, LBP expression was closely associated with T-classification, node lymph metastasis and clinical stage (P<0.01) but not with the pathological grading, sex and age of patients (P>0.1). Meanwhile, it was found that CD204 had the similar results in OSCC through immunohistochemistry staining assay. The LBP serum level in patients with OSCC abnormally increased compared with that in normal individuals (P<0.001). LBP serum concentration was highly correlated to T-classification and clinical stage (P<0.001). Area under the ROC was 0.876 (P<0.001), and the optional cut-off value was 0.733 μg·mL -1 with 93.3% specificity and 80.0% sensitivity. Conclusion LBP contributes to the tumourigenesis and development of OSCC and could become a promising biomarker that might provide a useful clinical value in the early diagnosis of OSCC.

Key words: lipopolysaccharide binding protein, oral squamous cell carcinoma, immunohistochemical double-stai-ning, enzyme linked immunosorbent assay, receiver operating characteristic curve

CLC Number: 

  • R782.2

TrendMD: 

Tab 1

Association between the patient’s clinicopathological characteristics and LBP expression in OSCC patients"

患者参数 病例数 CD204 P值 LBP P值
性别 1.000 0.45
男 28 4.95(3.63~6.4) 1.32±0.54
女 20 4.93±0.92 1.15(0.90~1.56)
年龄/岁 0.788 0.649
<60 24 5.07±1.27 1.29±0.42
≥60 24 4.90(3.85~6.38) 1.31±0.53
临床分期 0.001 0.007
Ⅰ 12 3.80(3.53~4.00) 1.05(0.90~1.30)
Ⅱ 24 5.28±1.29 1.25(0.85~1.59)
Ⅲ 12 5.65±1.13 1.75(1.20~2.20)
肿瘤分期 0.021 0.007
T1 14 3.85(3.57~4.60) 1.20(0.90~1.30)
T2 26 5.34±1.25 1.30(0.95~1.60)
T3 8 5.45±1.37 2.05(1.23~2.28)
病理分级 0.107 0.755
高分化 8 4.30±0.82 1.28±0.49
中分化 40 5.20(3.93~6.33) 1.30±0.48
淋巴结转移 0.007 0.039
N0 42 4.50(3.80~5.55) 1.24±0.45
N1-3 6 6.10(6.00~7.00) 1.70±0.50
组织来源 <0.001 <0.001
OSCC组织 48 4.90(3.93~6.08) 1.25(0.93~1.60)
癌旁组织 48 0.30(0.03~0.48) 0.05(0~0.20)

Fig 1

The overregulated expression of the LBP and CD204 protein in the OSCC tissues"

Fig 2

The LBP and CD204 protein expression in different T-classification and clinical stage of OSCC"

Fig 3

The LBP and CD204 protein expression in different histological grade and node lymph metastasis of OSCC"

Tab 2

Association between the patient’s clinicopathological characteristics and LBP serum level in OSCC patients"

患者参数 病例数 LBP/（μg·mL-1） P值
性别 0.848
男 80 1.57(1.26~2.22)
女 50 1.55(1.39~1.94)
年龄/岁 0.63
<60 56 1.57±0.50
≥60 74 1.76(1.35~2.15)
临床分期 <0.001
Ⅰ 28 1.37±0.24
Ⅱ 48 1.63±0.60
Ⅲ 44 1.78±0.59
Ⅳ 10 2.73(2.11~3.11)
肿瘤分期 <0.001
T1 36 1.35(1.25~1.55)
T2 68 1.63±0.57
T3 26 2.01(1.88~2.8)
病理分级 0.221
高分化 26 1.51(1.27~1.60)
中分化 96 1.74(1.31~2.07)
低分化 8 1.78(1.35~2.24)
淋巴结转移 0.176
N0 94 1.56(1.28~1.93)
N1-3 36 1.61(1.41~2.28)

Fig 4

The LBP and CD204 protein expression in different histological grade and node lymph metastasis of OSCC"

Fig 5

ROC curve for OSCC patients vs. normal group"

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