Int J Stomatol

Int J Stomatol ›› 2019, Vol. 46 ›› Issue (3): 277-281.doi: 10.7518/gjkq2019036

• Original Articles • Previous Articles     Next Articles

Research on the surface-enhanced Raman spectrum of blood serum for clinical staging of oral squamous cell carcinoma

Bing Yan1,2,3,Xianyang Luo1,2,3,Yingyun Tan4,Limei Guan1,Lili Xue5()   

  1. 1.Dept. of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China
    2.School of Medicine, Xiamen University, Xiamen 361003, China
    3.Xiamen Key Laboratory of Otolaryngology Head and Neck Surgery, Xiamen 361003, China
    4.Dept. of Implant, Stomatological Hospital of Chongqing Medical University, Chongqing 401120, China
    5.Dept. of Stomatology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China
  • Received:2018-09-21 Revised:2019-01-10 Online:2019-05-01 Published:2019-06-05
  • Contact: Lili Xue E-mail:xuelili0596@163.com
  • Supported by:
    This study was supported by National Natural Science Foundation of China(81502584);Project of Medical Innovation of Fujian Province(2015-CXB-35);Natural Science Foudation of Fujian Province(2017J01357);Project of Science and Technology of Xiamen(3502Z20174062)

RichHTML

8

PDF (PC)

291

Abstract:

Objective To evaluate the surface-enhanced Raman spectrum of the blood serum of oral squamous cell carcinoma patients and establish a diagnostic model for clinical staging. Methods Serum samples were obtained from a total of 144 patients with oral squamous cell carcinoma and classified into three groups, namely, the early cancer group, the mid-term cancer group, and the advanced cancer group. Principal component and linear discriminant analyses were utilized to establish a diagnostic model with which to classify the different groups based on spectral differences. Results A total of 144 spectra were generated from the samples. The main differences found involved peaks at 721-727, 931-932, 1 129-1 133,1 264-1 266, and 1 367-1 371 cm -1, which can be assigned to proteins, lipids, and nucleic acids. The total accuracy of the established model exceeded 80%, and its cross-validation accuracy exceeded 70%. Conclusion Surface-enhanced Raman spectroscopy could be applied to detect the clinical stages of oral squamous cell carcinoma based on blood serum.

Key words: oral squamous cell carcinoma, surface-enhanced Raman spectroscopy, clinical stage, diagnosis

CLC Number: 

  • R782

TrendMD: 

Fig 1

Scanning electron microscopy image of the gold nanoparticles"

Fig 2

Surface spectrums of serum"

Fig 3

The surface-enhanced Raman spectrums of serum of oral squamous cell carcinoma"

Tab 1

Classification result of the principle component analysis and linear discriminant analysis"

分类 预测分类 合计
早期组 中期组 晚期组
判别函数 早期组 44 7 8 59
中期组 5 35 0 40
晚期组 5 3 37 45
交互验证 早期组 37 11 11 59
中期组 7 32 1 40
晚期组 9 4 32 45

Fig 4

The scatter plot diagram"

[1] Blatt S, Krüger M, Ziebart T , et al. Biomarkers in diagnosis and therapy of oral squamous cell carcinoma: a review of the literature[J]. J Craniomaxillofac Surg, 2017,45(5):722-730.
doi: 10.1016/j.jcms.2017.01.033 pmid: 28318929
[2] Kreppel M, Nazarli P, Grandoch A , et al. Clinical and histopathological staging in oral squamous cell carcinoma-comparison of the prognostic significance[J]. Oral Oncol, 2016,60:68-73.
doi: 10.1016/j.oraloncology.2016.07.004 pmid: 27531875
[3] 薛丽丽, 李一, 蔡巧玲 , 等. 口腔黏膜鳞状细胞癌、上皮异常增生及正常黏膜组织拉曼光谱研究[J]. 中华口腔医学杂志, 2015,50(1):18-22.
doi: 10.3760/cma.j.issn.1002-0098.2015.01.005
Xue LL, Li Y, Cai QL , et al. Raman spectral characteristics of oral squamous cell carcinoma epithelial dysplasia and normal mucosa[J]. Chin J Stomatol, 2015,50(1):18-22.
doi: 10.3760/cma.j.issn.1002-0098.2015.01.005
[4] Ghantous Y, Bahouth Z , Abu El-Naaj I . Clinical and genetic signatures of local recurrence in oral squamous cell carcinoma[J]. Arch Oral Biol, 2018,95:141-148.
doi: 10.1016/j.archoralbio.2018.07.018
[5] Xue L, Yan B, Li Y , et al. Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma[J]. Int J Nanomedicine, 2018,13:4977-4986.
doi: 10.2147/IJN
[6] National Comprehensive Cancer Network. The NCCN clinical practice guidelines in oncology[EB/OL].. [2018-08-05]
[7] Wang J, Lin D, Lin J , et al. Label-free detection of serum proteins using surface-enhanced Raman spectroscopy for colorectal cancer screening[J]. J Biomed Opt, 2014,19(8):087003.
doi: 10.1117/1.JBO.19.8.087003 pmid: 25138208
[8] Yan B, Li B, Wen Z , et al. Label-free blood serum detection by using surface-enhanced Raman spectroscopy and support vector machine for the preoperative diagnosis of parotid gland tumors[J]. BMC Cancer, 2015,15:650.
doi: 10.1186/s12885-015-1653-7 pmid: 4595250
[9] Tan Y, Yan B, Xue L , et al. Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for the diagnosis of the oral squamous cell carcinoma[J]. Lipids Health Dis, 2017,16(1):73.
doi: 10.1186/s12944-017-0465-y pmid: 5384146
[10] Fleischmann M, Hendra PJ , McQuillan AJ . Raman spectra of pyridine adsorbed at a silver electrode[J]. Chem Phys Lett, 1974,26(2):163-166.
doi: 10.1016/0009-2614(74)85388-1
[11] Lin D, Pan J, Huang H , et al. Label-free blood plasma test based on surface-enhanced Raman scattering for tumor stages detection in nasopharyngeal cancer[J]. Sci Rep, 2014,4:4751.
doi: 10.1038/srep04751 pmid: 3996462
[12] Lin JS, Sun FJ, Lin PY , et al. Clinicopathological and prognostic significance of preoperative serum epidermal growth factor levels in patients with oral squamous cell carcinoma[J]. Int J Oral Maxillofac Surg, 2018,47(10):1236-1242.
doi: 10.1016/j.ijom.2018.01.023
[13] Gupta A, Tripathi A, Patil R , et al. Estimation of salivary and serum basic fibroblast growth factor in treated and untreated patients with oral squamous cell carcinoma[J]. J Oral Biol Craniofac Res, 2019,9(1):19-23.
doi: 10.1016/j.jobcr.2018.08.005
[14] Rekha P, Aruna P, Bharanidharan G , et al. Near infrared Raman spectroscopic characterization of blood plasma of normal, oral premalignant and malignant conditions—a pilot study[J]. J Raman Spectrosc, 2015,46(9):735-743.
doi: 10.1002/jrs.4693
[15] Sahu A, Nandakumar N, Sawant S , et al. Recurrence prediction in oral cancers: a serum Raman spectroscopy study[J]. Analyst, 2015,140(7):2294-2301.
doi: 10.1039/C4AN01860E
[1] Zhou Jinkuo,Zhang Jinhong,Shi Xiaojing,Liu Guangshun,Jiang Lei,Liu Qianfeng. Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation, invasion and migration of oral squamous carcinoma cells by regulating microRNA-27b-3p [J]. Int J Stomatol, 2024, 51(1): 52-59.
[2] Li Liheng,Wang Rui,Wang Xiaoming,Zhang Zhiyi,Zhang Xuan,An Feng,Wang Qin,Zhang Fan. Effects of circular RNA hsa_circ_0085576 on cell migration and invasion of oral squamous cell carcinoma by regulating the microRNA-498/B-cell-specific Moloney murine leukemia virus integration site 1 axis [J]. Int J Stomatol, 2024, 51(1): 60-67.
[3] Wu Jiamin,Xia Bin,Yang Hefeng,Xu Biao.. Research progress on cancer-associated fibroblasts in the tumor microenvironment of oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(6): 711-717.
[4] Liu Jianglong, Tuerdi Maimaitituxun. Progress of contrast-enhanced ultrasound in the diagnosis of cervical lymph node metastasis from oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(5): 514-520.
[5] Li Yijun, Xu Ziang, Li Yi.. Research progress on the sentinel lymph nodes in the detection of head and neck squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(5): 521-527.
[6] Ji Xiao,Zhang Lan,Huang Dingming.. Diagnosis and treatment of odontogenic and non-odontogenic maxillary sinusitis [J]. Int J Stomatol, 2023, 50(5): 566-572.
[7] Jiang Yueying,He Yutian,Li Ting,Zhou Ronghui.. Research progress on the application of near infrared fluorescence probe in the diagnosis of oral cancer [J]. Int J Stomatol, 2023, 50(4): 407-413.
[8] Xia Weiyao,Luo Yankun,Jia Zhonglin. Review of the precise diagnosis and genetic etiology of Pierre Robin sequence [J]. Int J Stomatol, 2023, 50(3): 287-292.
[9] Sheng Nanning,Wang Jue,Nan Xinrong. Research progress on mechanism and treatment of sex-determining region Y box 9 in oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(3): 314-320.
[10] Li Tan,Liang Xin-hua.. Role of discoidin domain receptor 1 in the regulation of malignant tumor progression and therapy [J]. Int J Stomatol, 2023, 50(2): 230-236.
[11] Qin Yichun,Tan Xuelian,Huang Dingming.. Clinical research progress on glandular odontogenic cyst [J]. Int J Stomatol, 2023, 50(1): 100-107.
[12] Li Ting,Yang Xuecai,Wang Junwei.. Children with Williams-Beuren syndrome associated with cranial and maxillofacial deformity [J]. Int J Stomatol, 2023, 50(1): 108-113.
[13] Li Weiguang,Wu Yafei,Guo Shujuan.. Research progress on the use of inorganic nanoparticles in the diagnosis and treatment of periodontal disease [J]. Int J Stomatol, 2022, 49(6): 724-730.
[14] Zhu Xingrong,Liao Lan. Research progress on oral clinical diagnosis and treatment of ectodermal dysplasia syndrome [J]. Int J Stomatol, 2022, 49(6): 737-742.
[15] Zeng Yanglin,Tan Xuelian,Song Dongzhe,Huang Dingming.. Research progress on diagnosis and treatment of root resorption [J]. Int J Stomatol, 2022, 49(5): 561-568.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[9] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[10] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .