Int J Stomatol

Int J Stomatol ›› 2015, Vol. 42 ›› Issue (6): 664-667.doi: 10.7518/gjkq.2015.06.012

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Similarity analysis of the oral bacteria community by polymerase chain reaction-denaturing gradient gel electrophoresis

Yu Miao1, Yi Hong2, Guo Xiao3.   

  1. 1. Dept. of Stomatology, Weifang People’s Hospital, Weifang 261041, China; 2. Dept. of Conservative Dentistry and Endodontics, The Affiliated Stomatological Hospital, Harbin Medical University, Harbin 150001, China; 3. Dept. of Stomatology, The Fifth Hospital of Daqing, Daqing 163000, China
  • Received:2015-04-15 Revised:2015-08-25 Online:2015-11-01 Published:2015-11-01

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Abstract:

Objective This study aimed to analyze the similarity of the oral bacteria community in 30 mother-child dyads via polymerase chain reaction(PCR)-based denaturing gradient gel electrophoresis. Methods Unstimulated saliva samples were obtained from 30 mother-child pairs:10 in caries-susceptive group, 10 in caries-moderate group, and 10 in caries-free group. DNAs were extracted for PCR amplification and denaturing gradient gel electrophoresis analysis. Results The compositions of the oral bacteria community in mother and child have a high degree of similarity. The oral bacteria community of children matched that of their mothers. The dice similarity coefficient of mother-child in the cariesfree group was more statistically significant compared with the two other groups(P<0.05). However, mothers of cariessusceptive children had significantly higher decay missing filling surface(DMFS) scores than mothers of caries-free and caries-moderate children(P<0.05). Conclusion Children obtained oral bacteria from their mothers.

Key words: saliva, oral, bacteria community, denaturing gradient gel electrophoresis

CLC Number: 

  • R 782

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