Int J Stomatol

Int J Stomatol ›› 2025, Vol. 52 ›› Issue (3): 349-357.doi: 10.7518/gjkq.2025046

• Original Articles • Previous Articles    

Promotion of mandibular defect healing through the regulation of osteogenic and angiogenic functions by sirtuin 1

Zhikai Liu1(),Hanghang Liu2,Shibo Liu1,Bolun Li1,Yao Liu1,En Luo1()   

  1. 1.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Orthognathic and Joint Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Emergency, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2024-07-19 Revised:2024-12-01 Online:2025-05-01 Published:2025-04-30
  • Contact: En Luo E-mail:liuzhikai2015@qq.com;luoen521125@sina.com
  • Supported by:
    National Natural Science Foundation of China(82370932);Key Research and Development Project of Sichuan Province(2024YFFK0204);Natural Science Foundation Youth Project of Sichuan Province(2023NSFSC1512)

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Abstract:

Objective This study aims to investigate the effect of sirtuin 1 (SIRT1) on osteogenic and angiogenic functions in mice under in vivo and in vitro conditions, as well as its effect on mandibular defect healing. Methods SIRT1 activators and inhibitors were used to intervene in MC3T3-E1 cells and mandibular defects in mice. Various methods, including cell counting kit-8 (CCK-8) assay, real-time quantitative polymerase chain reaction, Western blot, alkaline phosphatase staining, and immunofluorescence staining, were employed to study the influence of SIRT1 on the expression of osteogenic and angiogenic factors in MC3T3-E1 cells, as well as the healing and osteogenic and angiogenic functions of mandibular defects in mice. Results In in vitro experiments, the activation of SIRT1 promo-ted the expression of osteogenic and angiogenic factors in MC3T3-E1 cells. In in vivo experiments, SIRT1 activation facilitated the hea-ling of mandibular defects and enhanced the osteogenic and angiogenic functions of the mandibular defects. Conversely, the inhibition of SIRT1 activity suppressed the aforementioned processes. Conclusion SIRT1 can promote the healing of mandibular defects by regulating the osteogenic and angiogenic functions in mice.

Key words: sirtuin 1, angiogenesis, mandibular defect, bone regeneration

CLC Number: 

  • R782

TrendMD: 

Tab 1

Primer sequences of osteogenesis and angiogenesis-related genes"

基因引物序列F（5’-3’）引物序列R（5’-3’）
ALPCCAACTCTTTTGTGCCAGAGAGGCTACATTGGTGTTGAGCTTTT
Runx2TTCAACGATCTGAGATTTGTGGGGGATGAGGAATGCGCCCTA
VEGFCTGCCGTCCGATTGAGACCCCCCTCCTTGTACCACTGTC
Slit3TGCCCCACCAAGTGTACCTGGCCAGCGAAGTCCATTTTG
GAPDHAGGTCGGTGTGAACGGATTTGTGTAGACCATGTAGTTGAGGTCA

Fig 1

Effects of SRT1720 and EX527 on proliferation of MC3T3-E1 cells"

Fig 2

The effects of SIRT1 activators and inhibitors on the expression of osteogenic and angiogenic factors in MC3T3-E1 cells at 3 days"

Fig 3

The effects of SIRT1 activators and inhibitors on the expression of osteogenic and angiogenic factors in MC3T3-E1 cells at 7 days"

Fig 4

Effect of SIRT1 activators and inhibitors on ALP staining of MC3T3-E1 cells at 7 days"

Fig 5

Effect of SIRT1 activator and inhibitor on mandibular defect healing at 7 days"

Fig 6

Effects of SIRT1 activators and inhibitors on the expression of osteogenic and angiogenic factors in mandibular defects at 7 days"

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