Int J Stomatol

Inter J Stomatol ›› 2011, Vol. 38 ›› Issue (5): 535-538.doi: 10.3969/j.issn.1673-5749.2011.05.011

Previous Articles     Next Articles

γ-irradiation up-regulates the expression of mtDNA damage repair-related factors

Zhu Yang, Li Hejia, Li Dechao, Liu Jiguang.   

  1. Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154004, China
  • Received:2011-04-20 Revised:2011-06-21 Online:2011-09-20 Published:2011-09-20

RichHTML

4

PDF (PC)

949

Abstract:

Objective To study mitochondrial transcription factor A（Tfam）, 8-oxoguanine DNA glycosylase（Ogg）-1 and DNA polymerase gamma（Polg）, which is relative to mitochondrial DNA repair system, regulated by γ-irradiation in oral squamous cell carcinoma（OSCC）. Methods OSCC-2 and 5 cells were cultured in vitro and treated with γ-irradiation. Cell survival rate was calculated with methyl thiazolyl tetrazolium assay. Using Western blot, PCR and RT-PCR detected the protein and mRNA level of Tfam, Ogg-1 and Polg. Results After OSCC-2 and 5 cells were irradiated at a dose of 30 Gy, the cell proliferation were （35.33±6.03）% and （75.67±4.16）% respectively. The level of tfam mRNA were increased, but higher tfam mRNA levels were observed in OSCC-5 cells at 6 h after irradiation. Besides, the expression of Ogg-1 and Polg was also stronger in the irradiated OSCC-5 cells. In addition, each small interfering RNA（siRNA） down-regulated the corresponding protein expression. Transfection of the siRNA increased apoptosis of the irradiated cells. Conclusion In vitro, γ-irradiation can inhibit the proliferation and up-regulate the expression of Tfam, Ogg-1 and Polg of OSCC-2 and 5 cells.

Key words: oral squamous cell carcinoma, mitochondrial DNA, mitochondrial transcription factor A, 8-oxoguanine DNA glycosylase, DNA polymerase gamma


TrendMD: 
[1] Zhou Jinkuo,Zhang Jinhong,Shi Xiaojing,Liu Guangshun,Jiang Lei,Liu Qianfeng. Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation, invasion and migration of oral squamous carcinoma cells by regulating microRNA-27b-3p [J]. Int J Stomatol, 2024, 51(1): 52-59.
[2] Li Liheng,Wang Rui,Wang Xiaoming,Zhang Zhiyi,Zhang Xuan,An Feng,Wang Qin,Zhang Fan. Effects of circular RNA hsa_circ_0085576 on cell migration and invasion of oral squamous cell carcinoma by regulating the microRNA-498/B-cell-specific Moloney murine leukemia virus integration site 1 axis [J]. Int J Stomatol, 2024, 51(1): 60-67.
[3] Wu Jiamin,Xia Bin,Yang Hefeng,Xu Biao.. Research progress on cancer-associated fibroblasts in the tumor microenvironment of oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(6): 711-717.
[4] Liu Jianglong, Tuerdi Maimaitituxun. Progress of contrast-enhanced ultrasound in the diagnosis of cervical lymph node metastasis from oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(5): 514-520.
[5] Sheng Nanning,Wang Jue,Nan Xinrong. Research progress on mechanism and treatment of sex-determining region Y box 9 in oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(3): 314-320.
[6] Li Tan,Liang Xin-hua.. Role of discoidin domain receptor 1 in the regulation of malignant tumor progression and therapy [J]. Int J Stomatol, 2023, 50(2): 230-236.
[7] Zhao Zhuoping,Xin Pengfei,Gao Yang,Zhang Caifeng,Zhang Kuanshou,Liu Qingmei. Research progress on the use of photothermal therapy to treat oral squamous cell carcinoma [J]. Int J Stomatol, 2022, 49(4): 462-470.
[8] Jiang Han,Shen Yingqiang,Chen Qianming. Experimental study of muscarinic receptors on the biological behavior of oral squamous cell carcinoma through Yes related protein signal [J]. Int J Stomatol, 2022, 49(2): 138-143.
[9] Jiang Yulei,Xia Bin,Rao Nanquan,Yang Hefeng,Xu Biao. Exosomes mediate the malignant progression of oral squamous cell carcinoma and its application in diagnosis and treatment [J]. Int J Stomatol, 2021, 48(6): 711-717.
[10] Gan Jianguo,Gao Pan,Wang Xiaoyi. Research progress on the relationship between circulating tumor cells and oral squamous cell carcinoma [J]. Int J Stomatol, 2021, 48(2): 205-212.
[11] Huang Junwen,Qiao Jie,Mei Zi,Chen Zhuo,Li Yang,Qiao Bin. Expression and clinical significance of lipopolysaccharide binding protein in oral squamous cell carcinoma [J]. Int J Stomatol, 2021, 48(1): 50-57.
[12] He Yuqing,Dan Hongxia,Chen Qianming. Application of photodynamic therapy for oral carcinogenesis prevention [J]. Int J Stomatol, 2020, 47(6): 669-676.
[13] Hao Fu,Ning Yi,Sun Rui,Zheng Xiaoxu. Expression and potential clinical significance of Transformer 2β in oral squamous cell carcinoma [J]. Int J Stomatol, 2020, 47(2): 159-165.
[14] Xue Lingli,Li Yadong. Survival analysis of patients with oral squamous cell carcinoma treated by radical surgery for the first time [J]. Int J Stomatol, 2020, 47(2): 166-174.
[15] Chen Hongli,Yang Jing,Yin Gang,Li Haoyuan,Qiao Yan. Expression of zinc finger protein 32 in oral squamous cell carcinoma and its effect on oral squamous cell carcinoma stem cells [J]. Int J Stomatol, 2019, 46(6): 631-639.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 2005, 32(06): 458 -460 .
[8] . [J]. Foreign Med Sci: Stomatol, 2005, 32(06): 452 -454 .
[9] . [J]. Inter J Stomatol, 2008, 35(S1): .
[10] . [J]. Inter J Stomatol, 2008, 35(S1): .