Int J Stomatol

Int J Stomatol ›› 2021, Vol. 48 ›› Issue (1): 50-57.doi: 10.7518/gjkq.2021013

• Original Articles • Previous Articles     Next Articles

Expression and clinical significance of lipopolysaccharide binding protein in oral squamous cell carcinoma

Huang Junwen1,Qiao Jie2,Mei Zi1,Chen Zhuo1,Li Yang1,Qiao Bin1()   

  1. 1. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
    2. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
  • Received:2020-05-26 Revised:2020-09-16 Online:2021-01-01 Published:2021-01-20
  • Contact: Bin Qiao E-mail:qiaobin@zzu.edu.cn
  • Supported by:
    This study was supported by Project of Department of Science and Technology of Henan Province(182300410319)

RichHTML

15

PDF (PC)

115

Abstract:

Objective To investigate the expression and clinical importance of lipopolysaccharide binding protein(LBP) in oral squamous cell carcinoma (OSCC). Methods LBP expression in 48 OSCC tissues and matched adjacent normal tissues and LBP serum levels in 130 patients with OSCC and 90 normal individuals were measured by immunohistochemistry staining assay and enzyme-linked immunosorbent assay, respectively. The relationship between LBP expression and the clinicopathologic characteristics of OSCC was analysed by statistical methods. The diagnostic value of LBP serum level in OSCC was explored via receiver operating characteristic (ROC) curve. Results LBP expression was significantly upregulated in OSCC tissues, whereas little was expressed in adjacent normal tissues (P<0.001). Furthermore, LBP expression was closely associated with T-classification, node lymph metastasis and clinical stage (P<0.01) but not with the pathological grading, sex and age of patients (P>0.1). Meanwhile, it was found that CD204 had the similar results in OSCC through immunohistochemistry staining assay. The LBP serum level in patients with OSCC abnormally increased compared with that in normal individuals (P<0.001). LBP serum concentration was highly correlated to T-classification and clinical stage (P<0.001). Area under the ROC was 0.876 (P<0.001), and the optional cut-off value was 0.733 μg·mL -1 with 93.3% specificity and 80.0% sensitivity. Conclusion LBP contributes to the tumourigenesis and development of OSCC and could become a promising biomarker that might provide a useful clinical value in the early diagnosis of OSCC.

Key words: lipopolysaccharide binding protein, oral squamous cell carcinoma, immunohistochemical double-stai-ning, enzyme linked immunosorbent assay, receiver operating characteristic curve

CLC Number: 

  • R782.2

TrendMD: 

Tab 1

Association between the patient’s clinicopathological characteristics and LBP expression in OSCC patients"

患者参数 病例数 CD204 P值 LBP P值
性别 1.000 0.45
男 28 4.95(3.63~6.4) 1.32±0.54
女 20 4.93±0.92 1.15(0.90~1.56)
年龄/岁 0.788 0.649
<60 24 5.07±1.27 1.29±0.42
≥60 24 4.90(3.85~6.38) 1.31±0.53
临床分期 0.001 0.007
Ⅰ 12 3.80(3.53~4.00) 1.05(0.90~1.30)
Ⅱ 24 5.28±1.29 1.25(0.85~1.59)
Ⅲ 12 5.65±1.13 1.75(1.20~2.20)
肿瘤分期 0.021 0.007
T1 14 3.85(3.57~4.60) 1.20(0.90~1.30)
T2 26 5.34±1.25 1.30(0.95~1.60)
T3 8 5.45±1.37 2.05(1.23~2.28)
病理分级 0.107 0.755
高分化 8 4.30±0.82 1.28±0.49
中分化 40 5.20(3.93~6.33) 1.30±0.48
淋巴结转移 0.007 0.039
N0 42 4.50(3.80~5.55) 1.24±0.45
N1-3 6 6.10(6.00~7.00) 1.70±0.50
组织来源 <0.001 <0.001
OSCC组织 48 4.90(3.93~6.08) 1.25(0.93~1.60)
癌旁组织 48 0.30(0.03~0.48) 0.05(0~0.20)

Fig 1

The overregulated expression of the LBP and CD204 protein in the OSCC tissues"

Fig 2

The LBP and CD204 protein expression in different T-classification and clinical stage of OSCC"

Fig 3

The LBP and CD204 protein expression in different histological grade and node lymph metastasis of OSCC"

Tab 2

Association between the patient’s clinicopathological characteristics and LBP serum level in OSCC patients"

患者参数 病例数 LBP/（μg·mL-1） P值
性别 0.848
男 80 1.57(1.26~2.22)
女 50 1.55(1.39~1.94)
年龄/岁 0.63
<60 56 1.57±0.50
≥60 74 1.76(1.35~2.15)
临床分期 <0.001
Ⅰ 28 1.37±0.24
Ⅱ 48 1.63±0.60
Ⅲ 44 1.78±0.59
Ⅳ 10 2.73(2.11~3.11)
肿瘤分期 <0.001
T1 36 1.35(1.25~1.55)
T2 68 1.63±0.57
T3 26 2.01(1.88~2.8)
病理分级 0.221
高分化 26 1.51(1.27~1.60)
中分化 96 1.74(1.31~2.07)
低分化 8 1.78(1.35~2.24)
淋巴结转移 0.176
N0 94 1.56(1.28~1.93)
N1-3 36 1.61(1.41~2.28)

Fig 4

The LBP and CD204 protein expression in different histological grade and node lymph metastasis of OSCC"

Fig 5

ROC curve for OSCC patients vs. normal group"

[1] Bray F, Ferlay J, Soerjomataram I , et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018,68(6):394-424.
[2] Siegel RL, Miller KD, Jemal A . Cancer statistics, 2019[J]. CA A Cancer J Clin, 2019,69(1):7-34.
[3] Grivennikov SI, Greten FR, Karin M . Immunity, inflammation, and cancer[J]. Cell, 2010,140(6):883-899.
[4] Hanahan D, Weinberg RA . Hallmarks of cancer: the next generation[J]. Cell, 2011,144(5):646-674.
doi: 10.1016/j.cell.2011.02.013 pmid: 21376230
[5] Schumann RR, Leong SR, Flaggs GW , et al. Structure and function of lipopolysaccharide binding protein[J]. Science, 1990,249(4975):1429-1431.
[6] Zweigner J, Schumann RR, Weber JR . The role of lipopolysaccharide-binding protein in modulating the innate immune response[J]. Microbes Infect, 2006,8(3):946-952.
doi: 10.1016/j.micinf.2005.10.006 pmid: 16483818
[7] Tsukamoto H, Takeuchi S, Kubota K , et al. Lipopolysaccharide (LPS)-binding protein stimulates CD 14-dependent Toll-like receptor 4 internalization and LPS-induced TBK1-IKKϵ-IRF3 Axis activation[J]. J Biol Chem, 2018,293(26):10186-10201.
[8] Ryu JK, Kim SJ, Rah SH , et al. Reconstruction of LPS transfer cascade reveals structural determinants within LBP, CD14, and TLR4-MD2 for efficient LPS recognition and transfer[J]. Immunity, 2017,46(1):38-50.
[9] Kopp F, Kupsch S, Schromm AB . Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: implications for a role of LBP in intracellular LPS-signaling[J]. Biochim Biophys Acta, 2016,1863(4):660-672.
[10] Boylan KL, Andersen JD, Anderson LB , et al. Quantitative proteomic analysis by iTRAQ(R) for the iden-tification of candidate biomarkers in ovarian cancer serum[J]. Proteome Sci, 2010,8:31.
[11] Kovacs G, Peterfi L, Farkas N , et al. Expression of inflammatory lipopolysaccharide binding protein (LBP) predicts the progression of conventional renal cell carcinoma‒a short report[J]. Cell Oncol (Dordr), 2017,40(6):651-656.
[12] Peterfi L, Yusenko MV, Kovacs G . IL6 shapes an inflammatory microenvironment and triggers the development of unique types of cancer in end-stage kidney[J]. Anticancer Res, 2019,39(4):1869-1874.
[13] Wang N, Song XG, Liu LS , et al. Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer[J]. Cancer Sci, 2018,109(5):1701-1709.
[14] Kouketsu A, Sato I, Oikawa M , et al. Regulatory T cells and M2-polarized tumour-associated macropha-ges are associated with the oncogenesis and progression of oral squamous cell carcinoma[J]. Int J Oral Maxillofac Surg, 2019,48(10):1279-1288.
[15] Yamagata Y, Tomioka H, Sakamoto K , et al. CD163-positive macrophages within the tumor stroma are associated with lymphangiogenesis and lymph node metastasis in oral squamous cell carcinoma[J]. J O-ral Maxillofac Surg, 2017,75(10):2144-2153.
[16] ASMRHaque, Moriyama M, Kubota K, et al. CD206 + tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production [J]. Sci Rep, 2019,9(1):14611.
[17] Wang B, Liu H, Dong XL , et al. High CD204 + tumor-infiltrating macrophage density predicts a poor prognosis in patients with urothelial cell carcinoma of the bladder [J]. Oncotarget, 2015,6(24):20204-20214.
[18] Kawachi A, Yoshida H, Kitano S , et al. Tumor-associated CD204 + M2 macrophages are unfavorable pro-gnostic indicators in uterine cervical adenocarcinoma [J]. Cancer Sci, 2018,109(3):863-870.
[19] Wang YP, Chen C, Hua QQ , et al. Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma[J]. Oncol Rep, 2017,37(3):1503-1510.
[20] Chen R, Luo FK, Wang YL , et al. LBP and CD14 polymorphisms correlate with increased colorectal carcinoma risk in Han Chinese[J]. World J Gastroenterol, 2011,17(18):2326-2331.
[21] Chang CM, Chia VM, Gunter MJ , et al. Innate immunity gene polymorphisms and the risk of colorectal neoplasia[J]. Carcinogenesis, 2013,34(11):2512-2520.
[22] Cai QY, Jiang JH, Jin RM , et al. The clinical significance of lipopolysaccharide binding protein in hepatocellular carcinoma[J]. Oncol Lett, 2020,19(1):159-166.
[23] Kubota K, Moriyama M, Furukawa S , et al. CD163 + CD204 + tumor-associated macrophages contribute to T cell regulation via interleukin-10 and PD-L1 production in oral squamous cell carcinoma [J]. Sci Rep, 2017,7(1):1755.
[24] Kurago ZB, Lam-ubol A, Stetsenko A, et al. Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation[J].Head Neck Pathol, 2008,2(1):1-12.
[25] Utispan K, Pugdee K, Koontongkaew S . Porphyromonas gingivalis lipopolysaccharide-induced ma-crophages modulate proliferation and invasion of head and neck cancer cell lines[J]. Biomed Pharmacother, 2018,101:988-995.
[1] Zhou Jinkuo,Zhang Jinhong,Shi Xiaojing,Liu Guangshun,Jiang Lei,Liu Qianfeng. Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation, invasion and migration of oral squamous carcinoma cells by regulating microRNA-27b-3p [J]. Int J Stomatol, 2024, 51(1): 52-59.
[2] Li Liheng,Wang Rui,Wang Xiaoming,Zhang Zhiyi,Zhang Xuan,An Feng,Wang Qin,Zhang Fan. Effects of circular RNA hsa_circ_0085576 on cell migration and invasion of oral squamous cell carcinoma by regulating the microRNA-498/B-cell-specific Moloney murine leukemia virus integration site 1 axis [J]. Int J Stomatol, 2024, 51(1): 60-67.
[3] Wu Jiamin,Xia Bin,Yang Hefeng,Xu Biao.. Research progress on cancer-associated fibroblasts in the tumor microenvironment of oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(6): 711-717.
[4] Liu Jianglong, Tuerdi Maimaitituxun. Progress of contrast-enhanced ultrasound in the diagnosis of cervical lymph node metastasis from oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(5): 514-520.
[5] Sheng Nanning,Wang Jue,Nan Xinrong. Research progress on mechanism and treatment of sex-determining region Y box 9 in oral squamous cell carcinoma [J]. Int J Stomatol, 2023, 50(3): 314-320.
[6] Li Tan,Liang Xin-hua.. Role of discoidin domain receptor 1 in the regulation of malignant tumor progression and therapy [J]. Int J Stomatol, 2023, 50(2): 230-236.
[7] Zhao Zhuoping,Xin Pengfei,Gao Yang,Zhang Caifeng,Zhang Kuanshou,Liu Qingmei. Research progress on the use of photothermal therapy to treat oral squamous cell carcinoma [J]. Int J Stomatol, 2022, 49(4): 462-470.
[8] Jiang Han,Shen Yingqiang,Chen Qianming. Experimental study of muscarinic receptors on the biological behavior of oral squamous cell carcinoma through Yes related protein signal [J]. Int J Stomatol, 2022, 49(2): 138-143.
[9] Jiang Yulei,Xia Bin,Rao Nanquan,Yang Hefeng,Xu Biao. Exosomes mediate the malignant progression of oral squamous cell carcinoma and its application in diagnosis and treatment [J]. Int J Stomatol, 2021, 48(6): 711-717.
[10] Gan Jianguo,Gao Pan,Wang Xiaoyi. Research progress on the relationship between circulating tumor cells and oral squamous cell carcinoma [J]. Int J Stomatol, 2021, 48(2): 205-212.
[11] He Yuqing,Dan Hongxia,Chen Qianming. Application of photodynamic therapy for oral carcinogenesis prevention [J]. Int J Stomatol, 2020, 47(6): 669-676.
[12] Hao Fu,Ning Yi,Sun Rui,Zheng Xiaoxu. Expression and potential clinical significance of Transformer 2β in oral squamous cell carcinoma [J]. Int J Stomatol, 2020, 47(2): 159-165.
[13] Xue Lingli,Li Yadong. Survival analysis of patients with oral squamous cell carcinoma treated by radical surgery for the first time [J]. Int J Stomatol, 2020, 47(2): 166-174.
[14] Chen Hongli,Yang Jing,Yin Gang,Li Haoyuan,Qiao Yan. Expression of zinc finger protein 32 in oral squamous cell carcinoma and its effect on oral squamous cell carcinoma stem cells [J]. Int J Stomatol, 2019, 46(6): 631-639.
[15] Bing Yan,Xianyang Luo,Yingyun Tan,Limei Guan,Lili Xue. Research on the surface-enhanced Raman spectrum of blood serum for clinical staging of oral squamous cell carcinoma [J]. Int J Stomatol, 2019, 46(3): 277-281.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[9] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[10] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .