国际口腔医学杂志

国际口腔医学杂志 ›› 2019, Vol. 46 ›› Issue (3): 277-281.doi: 10.7518/gjkq2019036

• 论著 • 上一篇    下一篇

血清表面增强拉曼光谱应用于口腔鳞状细胞癌患者临床分期的研究

闫冰1,2,3,骆献阳1,2,3,谭迎赟4,关丽梅1,薛丽丽5()   

  1. 1.厦门大学附属第一医院耳鼻咽喉头颈外科 厦门 361003
    2.厦门大学医学院 厦门 361003
    3.厦门市耳鼻咽喉头颈外科重点实验室 厦门 361003
    4.重庆医科大学口腔医院种植科 重庆 401120
    5.厦门大学附属第一医院口腔科 厦门 361003
  • 收稿日期:2018-09-21 修回日期:2019-01-10 出版日期:2019-05-01 发布日期:2019-06-05
  • 通讯作者: 薛丽丽 E-mail:xuelili0596@163.com
  • 作者简介:闫冰,副教授,博士,Email： yanbing_west@163.com
  • 基金资助:
    国家自然科学基金(81502584);福建省医学创新课题(2015-CXB-35);福建省自然科学基金(2017J01357);厦门市科技计划项目(3502Z20174062)

Research on the surface-enhanced Raman spectrum of blood serum for clinical staging of oral squamous cell carcinoma

Bing Yan1,2,3,Xianyang Luo1,2,3,Yingyun Tan4,Limei Guan1,Lili Xue5()   

  1. 1.Dept. of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China
    2.School of Medicine, Xiamen University, Xiamen 361003, China
    3.Xiamen Key Laboratory of Otolaryngology Head and Neck Surgery, Xiamen 361003, China
    4.Dept. of Implant, Stomatological Hospital of Chongqing Medical University, Chongqing 401120, China
    5.Dept. of Stomatology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China
  • Received:2018-09-21 Revised:2019-01-10 Online:2019-05-01 Published:2019-06-05
  • Contact: Lili Xue E-mail:xuelili0596@163.com
  • Supported by:
    This study was supported by National Natural Science Foundation of China(81502584);Project of Medical Innovation of Fujian Province(2015-CXB-35);Natural Science Foudation of Fujian Province(2017J01357);Project of Science and Technology of Xiamen(3502Z20174062)

RichHTML

5

PDF (PC)

82

摘要：

目的 应用表面增强拉曼光谱技术研究口腔鳞状细胞癌患者血清,并建立临床分期鉴别诊断模型。方法 收集144例口腔鳞状细胞癌患者血清,按照早期组、中期组及晚期组进行分组检测血清表面增强拉曼光谱,比较光谱差异,应用主成分分析法及线性判别函数法建立鉴别诊断模型,对不同分组光谱数据进行鉴别诊断。结果 共获得血清拉曼光谱144例,其中早期组59例,中期组40例及晚期组45例。血清光谱差异主要表现在721~727、931~932 、1 129~1 133、1 264~1 266、1 367~1 371 cm -1处谱峰强度差异,主要与血清中脂类、蛋白质及核酸成分和结构有关。鉴别诊断模型总体准确率达80%以上,经过交互验证后准确率为70%以上。结论 表面增强拉曼光谱检测口腔鳞状细胞癌患者血清为口腔鳞状细胞癌临床分期及预后判断提供了一种简便、准确的新方法。

关键词: 口腔鳞状细胞癌, 表面增强拉曼光谱, 临床分期, 诊断

Abstract:

Objective To evaluate the surface-enhanced Raman spectrum of the blood serum of oral squamous cell carcinoma patients and establish a diagnostic model for clinical staging. Methods Serum samples were obtained from a total of 144 patients with oral squamous cell carcinoma and classified into three groups, namely, the early cancer group, the mid-term cancer group, and the advanced cancer group. Principal component and linear discriminant analyses were utilized to establish a diagnostic model with which to classify the different groups based on spectral differences. Results A total of 144 spectra were generated from the samples. The main differences found involved peaks at 721-727, 931-932, 1 129-1 133,1 264-1 266, and 1 367-1 371 cm -1, which can be assigned to proteins, lipids, and nucleic acids. The total accuracy of the established model exceeded 80%, and its cross-validation accuracy exceeded 70%. Conclusion Surface-enhanced Raman spectroscopy could be applied to detect the clinical stages of oral squamous cell carcinoma based on blood serum.

Key words: oral squamous cell carcinoma, surface-enhanced Raman spectroscopy, clinical stage, diagnosis

中图分类号: 

  • R782

图 1

纳米级金溶胶扫描电镜图"

图 2

血清样本表面光谱"

图 3

口腔鳞状细胞癌患者血清样本的表面增强拉曼光谱图 A：不同分组的血清样本平均表面增强拉曼光谱;B：中期组与早期组的差异;C：晚期组与中期组的差异。"

表 1

主成分分析及线性判别函数分类结果"

分类 预测分类 合计
早期组 中期组 晚期组
判别函数 早期组 44 7 8 59
中期组 5 35 0 40
晚期组 5 3 37 45
交互验证 早期组 37 11 11 59
中期组 7 32 1 40
晚期组 9 4 32 45

图 4

散点图"

[1] Blatt S, Krüger M, Ziebart T , et al. Biomarkers in diagnosis and therapy of oral squamous cell carcinoma: a review of the literature[J]. J Craniomaxillofac Surg, 2017,45(5):722-730.
doi: 10.1016/j.jcms.2017.01.033 pmid: 28318929
[2] Kreppel M, Nazarli P, Grandoch A , et al. Clinical and histopathological staging in oral squamous cell carcinoma-comparison of the prognostic significance[J]. Oral Oncol, 2016,60:68-73.
doi: 10.1016/j.oraloncology.2016.07.004 pmid: 27531875
[3] 薛丽丽, 李一, 蔡巧玲 , 等. 口腔黏膜鳞状细胞癌、上皮异常增生及正常黏膜组织拉曼光谱研究[J]. 中华口腔医学杂志, 2015,50(1):18-22.
doi: 10.3760/cma.j.issn.1002-0098.2015.01.005
Xue LL, Li Y, Cai QL , et al. Raman spectral characteristics of oral squamous cell carcinoma epithelial dysplasia and normal mucosa[J]. Chin J Stomatol, 2015,50(1):18-22.
doi: 10.3760/cma.j.issn.1002-0098.2015.01.005
[4] Ghantous Y, Bahouth Z , Abu El-Naaj I . Clinical and genetic signatures of local recurrence in oral squamous cell carcinoma[J]. Arch Oral Biol, 2018,95:141-148.
doi: 10.1016/j.archoralbio.2018.07.018
[5] Xue L, Yan B, Li Y , et al. Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma[J]. Int J Nanomedicine, 2018,13:4977-4986.
doi: 10.2147/IJN
[6] National Comprehensive Cancer Network. The NCCN clinical practice guidelines in oncology[EB/OL].. [2018-08-05]
[7] Wang J, Lin D, Lin J , et al. Label-free detection of serum proteins using surface-enhanced Raman spectroscopy for colorectal cancer screening[J]. J Biomed Opt, 2014,19(8):087003.
doi: 10.1117/1.JBO.19.8.087003 pmid: 25138208
[8] Yan B, Li B, Wen Z , et al. Label-free blood serum detection by using surface-enhanced Raman spectroscopy and support vector machine for the preoperative diagnosis of parotid gland tumors[J]. BMC Cancer, 2015,15:650.
doi: 10.1186/s12885-015-1653-7 pmid: 4595250
[9] Tan Y, Yan B, Xue L , et al. Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for the diagnosis of the oral squamous cell carcinoma[J]. Lipids Health Dis, 2017,16(1):73.
doi: 10.1186/s12944-017-0465-y pmid: 5384146
[10] Fleischmann M, Hendra PJ , McQuillan AJ . Raman spectra of pyridine adsorbed at a silver electrode[J]. Chem Phys Lett, 1974,26(2):163-166.
doi: 10.1016/0009-2614(74)85388-1
[11] Lin D, Pan J, Huang H , et al. Label-free blood plasma test based on surface-enhanced Raman scattering for tumor stages detection in nasopharyngeal cancer[J]. Sci Rep, 2014,4:4751.
doi: 10.1038/srep04751 pmid: 3996462
[12] Lin JS, Sun FJ, Lin PY , et al. Clinicopathological and prognostic significance of preoperative serum epidermal growth factor levels in patients with oral squamous cell carcinoma[J]. Int J Oral Maxillofac Surg, 2018,47(10):1236-1242.
doi: 10.1016/j.ijom.2018.01.023
[13] Gupta A, Tripathi A, Patil R , et al. Estimation of salivary and serum basic fibroblast growth factor in treated and untreated patients with oral squamous cell carcinoma[J]. J Oral Biol Craniofac Res, 2019,9(1):19-23.
doi: 10.1016/j.jobcr.2018.08.005
[14] Rekha P, Aruna P, Bharanidharan G , et al. Near infrared Raman spectroscopic characterization of blood plasma of normal, oral premalignant and malignant conditions—a pilot study[J]. J Raman Spectrosc, 2015,46(9):735-743.
doi: 10.1002/jrs.4693
[15] Sahu A, Nandakumar N, Sawant S , et al. Recurrence prediction in oral cancers: a serum Raman spectroscopy study[J]. Analyst, 2015,140(7):2294-2301.
doi: 10.1039/C4AN01860E
[1] 李继遥,郑广宁. 原发性牙根纵裂的诊治策略[J]. 国际口腔医学杂志, 2020, 47(4): 373-382.
[2] 吴勇志,尧可,陈思宇,黄能文,王了. 多生牙及其诊断治疗[J]. 国际口腔医学杂志, 2020, 47(3): 311-317.
[3] 郝福,宁毅,孙睿,郑晓旭. 口腔鳞状细胞癌中转化因子2β的表达及潜在的临床意义[J]. 国际口腔医学杂志, 2020, 47(2): 159-165.
[4] 薛伶俐,李雅冬. 经首次根治性手术治疗口腔鳞状细胞癌患者的生存相关影响因素分析[J]. 国际口腔医学杂志, 2020, 47(2): 166-174.
[5] 董云梅,陶艳,周瑜. 口腔黏膜癌变过程中血清生化标志物的研究进展[J]. 国际口腔医学杂志, 2020, 47(1): 43-50.
[6] 陈宏丽,杨敬,尹刚,李皓缘,乔燕. 锌指蛋白32在口腔鳞状细胞癌中的表达意义及对口腔鳞状细胞癌干细胞的影响[J]. 国际口腔医学杂志, 2019, 46(6): 631-639.
[7] 冷沙,黄定明,高原,张岚,徐玮哲,傅裕杰. 牛牙症的诊断与治疗[J]. 国际口腔医学杂志, 2019, 46(5): 509-515.
[8] 刘小旭,马坚,石冰,黄永清. Pai综合征的研究进展[J]. 国际口腔医学杂志, 2019, 46(3): 339-342.
[9] 赵文俊,刘媛媛,郝晓琪,王凯利,任家银,郭文豪,郑广宁. 46例患者第三磨牙区多生牙的影像学分析[J]. 国际口腔医学杂志, 2019, 46(1): 20-25.
[10] 李群,关为群,张杨安,黄志超. 骨膜蛋白和p53在口腔白斑及鳞状细胞癌组织中的表达及意义[J]. 国际口腔医学杂志, 2019, 46(1): 5-11.
[11] 李媛媛,程斌,王韵. 长链非编码RNA lnc-p26090对口腔鳞状细胞癌细胞糖酵解及增殖的影响[J]. 国际口腔医学杂志, 2018, 45(6): 628-634.
[12] 王伊婷,何永文. 口腔鳞状细胞癌相关长链非编码RNA调控肿瘤细胞上皮-间充质转化的研究进展[J]. 国际口腔医学杂志, 2018, 45(6): 635-639.
[13] 方川,李雅冬. 微小RNA在口腔鳞状细胞癌中的研究进展[J]. 国际口腔医学杂志, 2018, 45(6): 646-651.
[14] 杜雪,瞿方,刘伟才. 三维虚拟牙科患者的建立及其在美学修复中的应用[J]. 国际口腔医学杂志, 2018, 45(6): 695-702.
[15] 李倩,张平,陈娇,曾昕,冯云. 唾液组学在口腔癌诊断中的应用[J]. 国际口腔医学杂志, 2018, 45(6): 710-715.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 张新春. 桩冠修复与无髓牙的保护[J]. 国际口腔医学杂志, 1999, 26(06): .
[2] 王昆润. 长期单侧鼻呼吸对头颅发育有不利影响[J]. 国际口腔医学杂志, 1999, 26(05): .
[3] 彭国光. 颈淋巴清扫术中颈交感神经干的解剖变异[J]. 国际口腔医学杂志, 1999, 26(05): .
[4] 杨凯. 淋巴化疗的药物运载系统及其应用现状[J]. 国际口腔医学杂志, 1999, 26(05): .
[5] 康非吾. 种植义齿下部结构生物力学研究进展[J]. 国际口腔医学杂志, 1999, 26(05): .
[6] 柴枫. 可摘局部义齿用Co-Cr合金的激光焊接[J]. 国际口腔医学杂志, 1999, 26(04): .
[7] 孟姝,吴亚菲,杨禾. 伴放线放线杆菌产生的细胞致死膨胀毒素及其与牙周病的关系[J]. 国际口腔医学杂志, 2005, 32(06): 458 -460 .
[8] 费晓露,丁一,徐屹. 牙周可疑致病菌对口腔黏膜上皮的粘附和侵入[J]. 国际口腔医学杂志, 2005, 32(06): 452 -454 .
[9] 赵兴福,黄晓晶. 变形链球菌蛋白组学研究进展[J]. 国际口腔医学杂志, 2008, 35(S1): .
[10] 庞莉苹,姚江武. 抛光和上釉对陶瓷表面粗糙度、挠曲强度及磨损性能的影响[J]. 国际口腔医学杂志, 2008, 35(S1): .