国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (3): 261-266.doi: 10.7518/gjkq.2018.03.003
张鑫, 汪成林, 杨静, 叶玲
Zhang Xin, Wang Chenglin, Yang Jing, Ye Ling
摘要： 牙髓干细胞是牙髓组织中具有自我更新能力及多向分化潜能的一类成体干细胞,在牙髓修复、牙齿再生以及骨组织工程中发挥重要作用。表观遗传是指在DNA序列不改变的前提下基因表达发生了可遗传的变化,主要包括DNA甲基化、组蛋白修饰、非编码RNA调控等。研究发现,通过调控基因的转录过程,表观调控在牙髓干细胞的各项生物学行为中起着重要作用。本文对牙髓干细胞的表观遗传调控进行综述。
中图分类号:
| [1] Gronthos S, Mankani M, Brahim J, et al.Postnatal human dental pulp stem cells (DPSCs) [2] Miura M, Gronthos S, Zhao M, et al.SHED: stem cells from human exfoliated deciduous teeth[J]. Proc Natl Acad Sci USA, 2003, 100(10):5807-5812. [3] 马子洋, 郭晓霞. 牙髓干细胞在再生医学中的应用研究与进展[J]. 中国组织工程研究, 2016, 20(19): 2872-2878. Ma ZY, Guo XX.Dental pulp stem cells in regenera-tive medicine: application and development[J]. J Clin Rehab Tissue Eng Res, 2016, 20(19):2872-2878. [4] Goldberg AD, Allis CD, Bernstein E.Epigenetics: a landscape takes shape[J]. Cell, 2007, 128(4):635-638. [5] Radhakrishnan R, Kabekkodu S, Satyamoorthy K.DNA hypermethylation as an epigenetic mark for oral cancer diagnosis[J]. J Oral Pathol Med, 2011, 40(9):665-676. [6] Sørensen AL, Jacobsen BM, Reiner AH, et al.Pro-moter DNA methylation patterns of differentiated cells are largely programmed at the progenitor stage[J]. Mol Biol Cell, 2010, 21(12):2066-2077. [7] Tarfiei G, Noruzinia M, Soleimani M, et al.ROR2 promoter methylation change in osteoblastic dif-ferentiation of mesenchymal stem cells[J]. Cell J, 2011, 13(1):11-15. [8] Zhang D, Li Q, Rao L, et al.Effect of 5-Aza-2’-de-oxycytidine on odontogenic differentiation of human dental pulp cells[J]. J Endod, 2015, 41(5):640-645. [9] Nakatsuka R, Nozaki T, Uemura Y, et al.5-Aza-2’- deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells[J]. Arch Oral Biol, 2010, 55(5):350-357. [10] Rao LJ, Yi BC, Li QM, et al.TET1 knockdown in-hibits the odontogenic differentiation potential of human dental pulp cells[J]. Int J Oral Sci, 2016, 8(2): 110-116. [11] Viré E, Brenner C, Deplus R, et al.The polycomb group protein EZH2 directly controls DNA methyla-tion[J]. Nature, 2006, 439(7078):871-874. [12] Dunaway K, Goorha S, Matelski L, et al.Dental pulp stem cells model early life and imprinted DNA methylation patterns[J]. Stem Cells, 2017, 35(4):981-988. [13] Strahl BD, Allis CD.The language of covalent his-tone modifications[J]. Nature, 2000, 403(6765):41-45. [14] Barski A, Cuddapah S, Cui K, et al.High-resolution profiling of histone methylations in the human ge-nome[J]. Cell, 2007, 129(4):823-837. [15] Hui T, A P, Zhao Y, et al. EZH2, a potential regula-tor of dental pulp inflammation and regeneration[J]. J Endod, 2014, 40(8):1132-1138. [16] Xu J, Yu B, Hong C, et al.KDM6B epigenetically regulates odontogenic differentiation of dental me-senchymal stem cells[J]. Int J Oral Sci, 2013, 5(4): 200-205. [17] Hoang M, Kim JJ, Kim Y, et al.Alcohol-induced suppression of KDM6B dysregulates the mineraliza-tion potential in dental pulp stem cells[J]. Stem Cell Res, 2016, 17(1):111-121. [18] 付晓茹, 杜娟范, 范志朋. 组蛋白去甲基化酶FBXL11抑制牙髓干细胞成骨和成牙本质分化[J]. 北京口腔医学, 2013, 21(5):241-244. Fu XR, Du JF, Fan ZP.Histone demethylase FBXL- 11 inhibited the osteogenic and dentinogenic dif-ferentiation potential of dental pulp stem cells[J].Beijing J Stomatol, 2013, 21(5):241-244. [19] Du J, Ma Y, Ma P, et al.Demethylation of epiregulin gene by histone demethylase FBXL11 and BCL6 corepressor inhibits osteo/dentinogenic differentia-tion[J]. Stem Cells, 2013, 31(1):126-136. [20] Dong R, Yao R, Du J, et al.Depletion of histone demethylase KDM2A enhanced the adipogenic and chondrogenic differentiation potentials of stem cells from apical papilla[J]. Exp Cell Res, 2013, 319(18): 2874-2882. [21] Hui T, Wang C, Chen D, et al.Epigenetic regulation in dental pulp inflammation[J]. Oral Dis, 2017, 23(1):22-28. [22] Liu HJ, Wang T, Li QM, et al.Knock-down of p300 decreases the proliferation and odontogenic diffe-rentiation potentiality of HDPCs[J]. Int Endod J, 2015, 48(10):976-985. [23] Wang T, Liu H, Ning Y, et al.The histone acetyl-transferase p300 regulates the expression of pluri-potency factors and odontogenic differentiation of human dental pulp cells[J]. PLoS One, 2014, 9(7):e102117. [24] Jin H, Park JY, Choi H, et al.HDAC inhibitor trichostatin A promotes proliferation and odontoblast differentiation of human dental pulp stem cells[J]. Tissue Eng Part A, 2013, 19(5/6):613-624. [25] Duncan HF, Smith AJ, Fleming GJ, et al.Histone deacetylase inhibitors induced differentiation and accelerated mineralization of pulp-derived cells[J]. J Endod, 2012, 38(3):339-345. [26] Duncan HF, Smith AJ, Fleming GJ, et al.Histone deacetylase inhibitors epigenetically promote re-parative events in primary dental pulp cells[J]. Exp Cell Res, 2013, 319(10):1534-1543. [27] Paino F, La Noce M, Tirino V, et al.Histone deace-tylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement[J]. Stem Cells, 2014, 32(1):279-289. [28] Kwon A, Park HJ, Baek K, et al.Suberoylanilide hydroxamic acid enhances odontoblast differentia-tion[J]. J Dent Res, 2012, 91(5):506-512. [29] Duncan HF, Smith AJ, Fleming GJ, et al.HDACi: cellular effects, opportunities for restorative dentistry[J]. J Dent Res, 2011, 90(12):1377-1388. [30] Kaikkonen MU, Lam MT, Glass CK.Non-coding RNAs as regulators of gene expression and epigene-tics[J]. Cardiovasc Res, 2011, 90(3):430-440. [31] 张萍, 朱聪惠, 张纲, 等. 人牙髓干细胞向成牙本质细胞定向分化过程中miRNAs表达谱筛选及鉴定[J]. 实用口腔医学杂志, 2011, 27(2):212-216. Zhang P, Zhu CH, Zhang G, et al.Screening and identification of the expression profile of microRNAs in human dental pulp stem cells during directional differentiation into odontoblasts[J]. J Pract Stomatol, 2011, 27(2):212-216. [32] Huang X, Xu S, Gao J, et al.miRNA expression pro-filing identifies DSPP regulators in cultured dental pulp cells[J]. Int J Mol Med, 2011, 28(4):659-667. [33] Yue J, Wu B, Gao J, et al.DMP1 is a target of let-7 in dental pulp cells[J]. Int J Mol Med, 2012, 30(2): 295-301. [34] Liu H, Lin H, Zhang L, et al.miR-145 and miR-143 regulate odontoblast differentiation through targeting Klf4 and Osx genes in a feedback loop[J]. J Biol Chem, 2013, 288(13):9261-9271. [35] Gay I, Cavender A, Peto D, et al.Differentiation of human dental stem cells reveals a role for micro-RNA-218[J]. J Periodontal Res, 2014, 49(1):110-120. [36] Song Z, Chen LL, Wang RF, et al.MicroRNA-135b inhibits odontoblast-like differentiation of human dental pulp cells by regulating Smad5 and Smad4[J]. Int Endod J, 2016. doi:10.1111/iej.12678. [37] Hara ES, Ono M, Eguchi T, et al.miRNA-720 con-trols stem cell phenotype, proliferation and differen-tiation of human dental pulp cells[J]. PLoS One, 2013, 8(12):e83545. [38] Zhong S, Zhang S, Bair E, et al.Differential expres-sion of microRNAs in normal and inflamed human pulps[J]. J Endod, 2012, 38(6):746-752. [39] Kong Q, Liu L, Huang Y, et al.The effect of oc-tamer-binding transcription factor 4B1 on microRNA signals in human dental pulp cells with inflammatory response[J]. J Endod, 2014, 40(1):101-108. [40] Wang MC, Hung PS, Tu HF, et al.Lipopolysaccharide induces the migration of human dental pulp cells by up-regulating miR-146a[J]. J Endod, 2012, 38(12): 1598-1603. [41] Gu S, Ran S, Liu B, et al.miR-152 induces human dental pulp stem cell senescence by inhibiting SIRT7 expression[J]. FEBS Lett, 2016, 590(8):1123-1131. |
| [1] | 王润婷,房付春. 非编码RNA调控人牙周膜干细胞成骨向分化的研究进展[J]. 国际口腔医学杂志, 2020, 47(2): 138-145. |
| [2] | 周婷茹,李永生. 牙髓干细胞成骨微环境的研究进展[J]. 国际口腔医学杂志, 2019, 46(6): 675-679. |
| [3] | 张凯莹,房付春,吴补领. 非编码RNA在牙源性干细胞成牙本质向分化中作用的研究进展[J]. 国际口腔医学杂志, 2019, 46(5): 540-545. |
| [4] | 原振英,管翠强,南欣荣. DNA甲基化与口腔疾病的研究进展[J]. 国际口腔医学杂志, 2019, 46(4): 437-441. |
| [5] | 李媛媛,程斌,王韵. 长链非编码RNA lnc-p26090对口腔鳞状细胞癌细胞糖酵解及增殖的影响[J]. 国际口腔医学杂志, 2018, 45(6): 628-634. |
| [6] | 王伊婷,何永文. 口腔鳞状细胞癌相关长链非编码RNA调控肿瘤细胞上皮-间充质转化的研究进展[J]. 国际口腔医学杂志, 2018, 45(6): 635-639. |
| [7] | 韩曈曈,陈乔尔,朱友明. p53相关长链非编码RNA及其与口腔癌的关系[J]. 国际口腔医学杂志, 2018, 45(5): 597-602. |
| [8] | 王丽萍, 查骏, 葛林虎. 非编码RNA在舌鳞状细胞癌中的研究进展[J]. 国际口腔医学杂志, 2018, 45(4): 420-424. |
| [9] | 宋洪宁, 王旭霞, 张君. 长链非编码RNA的研究进展及其与口腔肿瘤的关系[J]. 国际口腔医学杂志, 2018, 45(4): 425-432. |
| [10] | 葛逸弘, 房付春, 吴补领. 长链非编码RNA在间充质干细胞多向分化过程中的调节作用[J]. 国际口腔医学杂志, 2018, 45(3): 267-271. |
| [11] | 杨鑫, 李思洁, 赵玮. Wnt信号通路在调控牙髓干细胞多向分化及炎症损伤修复中的作用[J]. 国际口腔医学杂志, 2018, 45(3): 286-290. |
| [12] | 刘双, 李纾. 表观遗传学及其调控与牙周病[J]. 国际口腔医学杂志, 2017, 44(5): 523-527. |
| [13] | 杨懋彬1 曾倩2. 再生牙髓病学——牙髓再生的新方向[J]. 国际口腔医学杂志, 2016, 43(5): 495-499. |
| [14] | 陈冬茹 吴莉萍. 低氧诱导因子-1α和反义低氧诱导因子-1α的研究进展[J]. 国际口腔医学杂志, 2016, 43(5): 589-593. |
| [15] | 周晨,凌均棨. 表观遗传在牙发生和牙再生中的作用及意义[J]. 国际口腔医学杂志, 2016, 43(3): 318-324. |
|