国际口腔医学杂志

国际口腔医学杂志 ›› 2022, Vol. 49 ›› Issue (2): 220-226.doi: 10.7518/gjkq.2022033

• 综述 • 上一篇    下一篇

人牙髓干细胞无血清培养方法的研究进展

付恒怡(),汪成林()   

  1. 口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院牙体牙髓病科 成都 610041
  • 收稿日期:2021-06-06 修回日期:2021-10-05 出版日期:2022-03-01 发布日期:2022-03-15
  • 通讯作者: 汪成林
  • 作者简介:付恒怡,硕士,Email： 962726878@qq.com
  • 基金资助:
    四川省科技计划项目(2019YFS0035);四川大学华西口腔医院探索与研发项目(LCYJ2019-18)

Research progress on serum-free culture methods of human dental pulp stem cells and cell characterization

Fu Hengyi(),Wang Chenglin()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2021-06-06 Revised:2021-10-05 Online:2022-03-01 Published:2022-03-15
  • Contact: Chenglin Wang
  • Supported by:
    Sichuan Province Science and Technology Support Program(2019YFS0035);The Exploration and Creation Project of West China Hospital of Stomatology, Sichuan University(LCYJ2019-18)

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摘要：

人牙髓干细胞是再生医学中的主要细胞来源之一,具有多向分化潜能,已经被应用于治疗多种疾病,包括Ⅰ型糖尿病、神经系统疾病等。为满足临床需求,牙髓干细胞需进行体外扩增。常规的牙髓干细胞体外扩增方法需要运用添加胎牛血清的培养基,但近些年来由于胎牛血清存在伦理和安全两方面的问题,推荐使用无血清培养方法。本文对无血清培养基培养人牙髓干细胞的组成及方法,及其培育出的细胞的特性和其他相关应用进行综述。

关键词: 人牙髓干细胞, 无血清培养, 干细胞特性

Abstract:

Human dental pulp stem cells are a principal source in regenerative medicine. They have a property of multidirectional differentiation and have been used to treat various diseases, including type 1 diabetes and nervous system di-sease. Stem cells in dental pulp must be expanded in vitro to meet clinical needs. Conventional in vitro amplification me-thods require the use of medium supplemented with foetal bovine serum. However, serum-free culture methods are recommended because of the ethical and safety issues of fetal bovine serum. This review describes the composition of serum-free medium, the methods of cultivating human dental pulp stem cells and the characteristics of the cultured cells and o-ther related applications.

Key words: human dental pulp stem cells, serum-free culture, stem cell characterization

中图分类号: 

  • R34
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