国际口腔医学杂志

国际口腔医学杂志 ›› 2019, Vol. 46 ›› Issue (5): 540-545.doi: 10.7518/gjkq.2019075

• 综述 • 上一篇    下一篇

非编码RNA在牙源性干细胞成牙本质向分化中作用的研究进展

张凯莹1,房付春1,2,吴补领1,2()   

  1. 1. 南方医科大学南方医院口腔科 广州 510515
    2. 南方医科大学口腔医学院 广州 510515
  • 收稿日期:2018-11-18 修回日期:2019-05-18 出版日期:2019-09-01 发布日期:2019-09-10
  • 通讯作者: 吴补领
  • 作者简介:张凯莹,学士,Email： 804866294@qq.com
  • 基金资助:
    国家自然科学基金(81870755);国家自然科学基金(81600882)

Research progress on non-coding RNA in odontoblastic differentiation of dental tissue-derived stem cells

Zhang Kaiying1,Fang Fuchun1,2,Wu Buling1,2()   

  1. 1. Dept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    2. College of Stomatology, Southern Medical University, Guangzhou 510515, China
  • Received:2018-11-18 Revised:2019-05-18 Online:2019-09-01 Published:2019-09-10
  • Contact: Buling Wu
  • Supported by:
    This study was supported by National Natural Science Foundation of China(81870755);This study was supported by National Natural Science Foundation of China(81600882)

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摘要：

牙源性干细胞是从口腔组织中分离出来的成体干细胞,具有自我更新和多向分化的潜能。成牙本质向分化作为产生修复性牙本质的重要防御反应,其过程涉及一系列转录及转录后的基因表达调控。多项研究证实非编码RNA（ncRNA）在牙源性干细胞特别是牙髓干细胞成牙本质向分化过程中发挥了重要作用,是细胞分化的重要调控靶点,以维持细胞分化特性。新研究技术体系的建立使得ncRNA的调控机制得以进一步阐明。本文从功能、调控的靶基因及通路等方面,对目前ncRNA在牙源性干细胞成牙本质向分化过程中的相关研究进行综述。

关键词: 牙源性干细胞, 成牙本质向分化, 非编码RNA

Abstract:

Dental tissue-derived stem cells, adult stem cells obtained from dental tissues, exhibit self-renewal and multiple differentiation potentials. Odontoblastic differentiation, as an important generation procedure of reparative dentin, is modulated by many transcriptional and post-transcriptional factors. Non-coding RNAs (ncRNAs) are thought to play an important role in the odontoblastic differentiation of dental tissue-derived stem cells, especially dental pulp stem cells. ncRNAs are crucial to the regulation of differentiation and maintenance of cell differentiation characteristics. The developments in research technologies enable the regulatory mechanism of ncRNAs to be intensively and furtherly elucidated. Focusing on the function, regulation of target genes and pathways, we reviewed the mechanism of ncRNAs during the odontoblastic differentiation of dental tissue-derived stem cells.

Key words: dental tissue-derived stem cell, odontoblastic differentiation, non-coding RNA

中图分类号: 

  • Q254

表 1

ncRNA参与牙源性干细胞成牙本质向分化调控"

类型 名称 细胞 调控水平 表达调控 影响* 可能的靶基因或作用通路 参考文献
lncRNA DANCR 人DPSC 转录 上调 抑制 Wnt/β-连环蛋白 Chen等[14]
lncRNA H19 人DPSC 表观遗传 上调 促进 H19/SAHH途径调控DNMT3B介导的DLX3d基因 Zeng等[16]
甲基化和表达
lncRNA H19 人SCAP 转录 上调 促进 H19/SPAG9信号通路,H19作为ceRNA竞争性结 Li等[17]
合miR-141,调控MAPK信号通路
lncRNA STL 人DPSC 转录后 上调 促进 可能通过NQO1及ERO1L发挥调控作用 Shi等[18]
lncRNA - 鼠牙间充 转录后 108个上调,36 Zheng等[19]
质干细胞 个下调
miRNA miR-21 人DPSC 转录后 上调 促进 miR-21/STAT3信号通路 Xu等[25]
miRNA miR-223-3p 人DPSC 转录后 上调 促进 靶向作用于SMAD3,抑制DSPP、DMP1基因 Huang等[26]
miRNA miR-143-5p 人DPSC 转录后 下调 促进 OPG/RANKL信号通路,靶向作用于RUNX2 Zhan等[28]
miRNA miR-143-5p 人DPSC 转录后 下调 促进 靶向作用于MAPK14、p38 MAPK信号通路 Wang等[27]
miRNA miR-3065-5p 鼠DPSC 转录后 上调 促进 靶向作用于BMPR2 Lin等[30]
circRNA 鼠牙乳头 转录后 3 255个上调, 占云燕等[32]
细胞 809个下调
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