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Table of Content

    01 May 2018, Volume 45 Issue 3 Previous Issue    Next Issue
    Expert Forum
    Research progress on application of DNA origami in stem cell field
    Lin Yunfeng, Li Songhang
    Inter J Stomatol. 2018, 45 (3):  249-254.  DOI: 10.7518/gjkq.2018.03.001
    Abstract ( 894 )   HTML ( 31 )   PDF(pc) (1335KB) ( 214 )   Save
    DNA origami is a newly proposed DNA self-assembly method in recent years. It can design specific DNA sequences and follow the principle of complementary base pairing to construct more complex nanostructures and nanopatterns. The recent discovery of tetrahedral DNA nanostructure (TDN) constructed by DNA origami has great application potential in the field of stem cells. In this study, we reviewed the structure and biological properties of TDN, and the applications of TDN in the field of stem cells.
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    Stem Cell
    Effects of culture conditions in vitro on the biological characteristics of periodontal ligament stem cells
    Jia Linglu, Wen Yong, Xu Xin
    Inter J Stomatol. 2018, 45 (3):  255-260.  DOI: 10.7518/gjkq.2018.03.002
    Abstract ( 750 )   HTML ( 20 )   PDF(pc) (1333KB) ( 386 )   Save
    Periodontal ligament stem cell, which is a kind of adult stem cell, owns the abilities of self-renewing and multi-differentiation and has become the hot spot in the oral tissue engineering. When cultured in vitro, different culture conditions such as primary cell separation methods, culture medium composition, oxygen concentration, culture time, cell storage conditions and other physical or chemical stimulation can change the proliferation, immigration or differentiation characteristics of periodontal ligament stem cell. This paper is committed to review on the research progress regarding the effects of the culture conditions in vitro on the biological characteristics of periodontal ligament stem cell in recent years and provide theoretical reference for tissue engineering.
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    Epigenetic regulation in dental pulp stem cells
    Zhang Xin, Wang Chenglin, Yang Jing, Ye Ling
    Inter J Stomatol. 2018, 45 (3):  261-266.  DOI: 10.7518/gjkq.2018.03.003
    Abstract ( 832 )   HTML ( 12 )   PDF(pc) (1150KB) ( 692 )   Save
    Dental pulp stem cells (DPSCs) are adult stem cells with self-renewal ability and multiple differentiation potential. These cells play an important role in dental pulp repair, tooth regeneration, and bone tissue engineering. Epigenetics refers to heritable changes in gene expression without alteration of DNA sequence; these changes include DNA methylation, histone modification, and non-coding RNA regulation. Epigenetic modification plays an important role in regulation of DPSCs by influencing gene transcription. Thus, this study aims to elaborate epigenetic regulations of DPSCs.
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    Regulate process of long non-coding RNA in multi-differentiation of mesenchymal stem cells
    Ge Yihong, Fang Fuchun, Wu Buling
    Inter J Stomatol. 2018, 45 (3):  267-271.  DOI: 10.7518/gjkq.2018.03.004
    Abstract ( 715 )   HTML ( 5 )   PDF(pc) (1127KB) ( 210 )   Save
    Mesenchymal stem cells (MSCs) derived from mesoderm exhibit self-renewal and multiple differentiation potentials. MSCs are considerably useful in tissue repair and cell development, with the ability to differentiate into osteoblasts, chondrocytes, adipocytes, myoblasts, neurocytes and other cells. Long noncoding RNAs (lncRNAs) are differentially expressed in the differentiation of MSCs. This result indicated that lncRNAs are crucial in regulation during differentiation. To date, most reports mainly focused on microarray and bioinformatics analysis of lncRNAs. The function and regulatory mechanism of lncRNAs on the differentiation of MSCs still remain unclear. However, with the development in new research technologies, the regulatory mechanism of lncRNA in the differentiation of MSCs will be intensively elucidated.
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    Regulation of RNA N6-methylation of adenosine on stem cell differentiation
    Wu Yunshu, Yuan Quan
    Inter J Stomatol. 2018, 45 (3):  272-275.  DOI: 10.7518/gjkq.2018.03.005
    Abstract ( 621 )   HTML ( 4 )   PDF(pc) (1116KB) ( 382 )   Save
    N6-methylation of adenosine is the most prevalent post-transcriptional RNA modification in eukaryotes. Mettl3, Mettl14 and Wtap are methyltransferases that catalyse the formation of N6-methyladenosine (m6A). Demethylase, FTO and ALKBH5 can remove the methyl of m6A, thereby rendering a dynamic and reversible m6A modification. Methyltransferases and demethylases can regulate the pluripotency and differentiation of stem cells through writing and erasure of m6A marks on RNA. These data provide a novel dimension in epigenetic regulation at the RNA level to affect stem cell fate.
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    Immune regulation of dental stem cells
    Zhang Xiangyi, Liu Yali
    Inter J Stomatol. 2018, 45 (3):  276-279.  DOI: 10.7518/gjkq.2018.03.006
    Abstract ( 1017 )   HTML ( 16 )   PDF(pc) (1115KB) ( 504 )   Save
    Dental stem cell (DSC) is a type of stem cells that possess unique immunomodulation properties and can be effectively obtained from isolated dental or periodontal tissue. They exert immunosuppressive effects through direct contact or secretion of cytokines and play a key role in the proliferation, differentiation, and apoptosis of T cells, B cells, and other lymphocytes. The treatment of a variety of chronic inflammatory and autoimmune diseases has a certain clinical significance. In this review, we summarize current studies on the immunomodulatory properties of several common DSCs.
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    Characteristics of dental tissue-derived stem cells and their application in bone tissue engineering
    Zhou Jie, Wang Ying, Zhang Lei, Wu Tingting, Zhou Yong, Zou Duohong
    Inter J Stomatol. 2018, 45 (3):  280-285.  DOI: 10.7518/gjkq.2018.03.007
    Abstract ( 1315 )   HTML ( 29 )   PDF(pc) (1141KB) ( 607 )   Save
    In recent years, adult stem cells obtained from dental tissue, including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, apical papilla stem cells, periodontal ligament stem cells and follicle progenitor cells, provide sources of seed cells for oral tissue engineering in dentistry. In this paper, we review the characteristics of these adult stem cells and their applications in bone tissue engineering.
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    Wnt signaling pathway mediates the dental pulp stem cells in multipotential differentiation and inflammatory microenvironment
    Yang Xin, Li Sijie, Zhao Wei
    Inter J Stomatol. 2018, 45 (3):  286-290.  DOI: 10.7518/gjkq.2018.03.008
    Abstract ( 716 )   HTML ( 10 )   PDF(pc) (1134KB) ( 640 )   Save
    Human dental pulp stem cell (hDPSC) and stem cells from human exfoliated deciduous teeth (SHED) are human mesenchymal stem cells demonstrating multipotential differentiation capabilities. The Wnt signaling pathway consists of canonical Wnt/β-catenin and noncanonical Wnt signal pathways, which are activated during tooth morphogenesis. These pathways also play key roles in the dental pulp repair process. During inflammation, an activated Wnt signal pathway plays a significant role in the process involved in the repair of a damaged tooth. This pathway also participates in hDPSC proliferation and odontoblastic differentiation. In non-inflammatory conditions, hDPSC and SHED differentiate into osteoblasts and odontoblasts, respectively, which suppress adipogenic differentiation through the Wnt signaling pathway.
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    Radiology
    Stereophotogrammetry-based facial soft tissue measurement of Mongolian adults with normal occlusion
    Fan Yongjie, A Ronggaowa
    Inter J Stomatol. 2018, 45 (3):  291-294.  DOI: 10.7518/gjkq.2018.03.009
    Abstract ( 617 )   HTML ( 4 )   PDF(pc) (1356KB) ( 149 )   Save
    Objective This study aimed to establish reference data of facial soft tissue of Mongolian adults with normal occlusion of Inner Mongolia. Methods Sixty-eight male and 78 female students from Mongolian preparatory courses in Inner Mongolia universities were included in this study. Three-dimensional facial photos were obtained, and eight mark points were established on the software, and then the length of 12 line segments was measured. The obtained data were analyzed by double sample t-test to obtain reference data of facial soft tissue of Mongolian adults with normal occlusion. Results Statistical differences were found in terms of depth, height, width of forehead, upper face, and lower face between men and women (P<0.05). Conclusion This paper introduces a new method of using 3D stereophotogrammetry for measuring facial soft tissue. A database of facial soft tissue of Mongolian adults with normal occlusion was established.
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    Application progress on cone beam computed tomography imaging localization in extraction of embedded supernumerary teeth in children
    Liu Xiaohua, Wang Enbo
    Inter J Stomatol. 2018, 45 (3):  295-300.  DOI: 10.7518/gjkq.2018.03.010
    Abstract ( 869 )   HTML ( 13 )   PDF(pc) (1408KB) ( 413 )   Save
    The presence of supernumerary teeth in children affect the eruption of normal permanent teeth, resulting in malocclusion, and accompanied by destructive lesions such as cysts, which require early removal. Accurate localization of embedded supernumerary teeth during removal is critical. Under the guidance of accurate positioning, the surgical procedure can be correctly formulated and the appropriate surgical approach could be chosen to reduce the trauma of alveolar surgery. Over the past ten years, cone beam computed tomography (CBCT) has been the most advanced and accurate imaging technique in oral and alveolar surgery. At present, CBCT is an ideal technique for determining the location of impacted teeth. The three-dimensional images can be used to observe the relationship between the impacted teeth and their adjacent teeth in different axial directions. It can determine the distance between the bony plates of the labial and palatal sides of the impacted teeth. CBCT is more and more widely used in oral diagnosis and treatment, and has its unique advantages. This paper reviews the clinical application of CBCT in the localization of supernumerary teeth in children.
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    Research progress on the correction of natural head position with three-dimensional stereo-photography
    Yin Yijia, Feng Jie, Luo Mengqi, Han Xianglong
    Inter J Stomatol. 2018, 45 (3):  301-306.  DOI: 10.7518/gjkq.2018.03.011
    Abstract ( 809 )   HTML ( 10 )   PDF(pc) (1143KB) ( 375 )   Save
    With the growing importance of esthetic considerations, soft-tissue evaluation plays a crucial role in maxillofacial treatment, including the establishment of normative anthropometric database, treatment planning, and postoperative evaluation. In therapy, three-dimensional (3D) photography techniques with high reproducibility, such as cone beam computed tomography (CBCT), 3D stereo-photography, and laser scanning technology, can facilitate the comprehensive oral and maxillofacial assessment different from direct and 2D measurements. 3D photography is faster, more precise, more automatic and less harmful than the traditional methods. A universal standard for 3D-maxillofacial morphology has not been established, thus limiting the clinical applications of 3D stereo-photography. Natural head position is the most direct reference to maxillofacial assessment. However, the lack of a standardized method of correcting natural head position is the main factor that limits the use of 3D stereo-photography. Therefore, a versatile method for correcting accurate and reliable head position is needed. In this paper, several methods for recording and correcting the natural head position based on 3D stereo-photography were discussed. The difference between the correction procedure based on 3D stereo-photography and CBCT were compared, and the optimal approach for correcting natural head position in 3D scale was determined.
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    Case Report
    Aneurysmal bone cyst in mandibular condyle: two cases report
    Zhao Wenjun, Wang Kaili, Liu Li, Guo Wenhao, Zheng Guangning
    Inter J Stomatol. 2018, 45 (3):  307-312.  DOI: 10.7518/gjkq.2018.03.012
    Abstract ( 1186 )   HTML ( 10 )   PDF(pc) (3935KB) ( 339 )   Save
    Aneurysmal bone cyst (ABC) is a rare, expanding and osteolytic lesion which is neither cystic nor neoplastic disease. ABC rarely occurs in the craniofacial bone, and the lesions in mandibular condyle are scarce. In this paper, we report two cases in mandibular condyle of ABC which were confirmed by histopathology. The aim of this paper is to explore the radiographic and clinical features of ABC and improve the understanding of the ABC in mandibular condyle.
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    Original Articles
    Expression changes of platelet-derived growth factor receptor α in the process of palatal suspension culture of the C57BL/6J mouse in vitro
    Cong Wenwen, Zhang Daizun, Xiao Wenlin, Xue Lingfa, Xu Yaoxiang
    Inter J Stomatol. 2018, 45 (3):  313-318.  DOI: 10.7518/gjkq.2018.03.013
    Abstract ( 570 )   HTML ( 9 )   PDF(pc) (2111KB) ( 165 )   Save
    Objective This experiment builded in C57BL/6J mouse models of palatal process culture in vitro, to study the expression of platelet-derived growth factor receptor α (PDGFR-α) during different times. Methods Experiment cultured palates with the method of suspension culture for 24, 36, 48 h, palatal development was observed by stereomicroscope and hematoxylin-eosin (HE) staining. PDGFR-α expression location in different period was detected by immunohistochemical assays. The expression of PDGFR-α was examined by Western blot. Results Stereomicroscope and HE staining results showed that in vitro cultivation of 24 h, palatal process the space between bilateral palatal process reduced, bilateral palatal process contact and the crest epithelial seam could be seen in stereomicroscope and HE in 36 h, bilateral palatal process fusion and the crest epithelial seam disappearance could be seen in stereomicroscope and HE in 48 h. The expression level of PDGFR-α cultured palates for 24 h reached its peak and gradually reduced in 36 and 48 h. Conclusion The changes of PDGFR-α expression in cultivation of palatal process in vitro and in vivo had the same trend. In this study, improved method of suspension culture of palatal process in vitro simulated the development of the palatal process in vivo. The study had made a foundation for subsequent cleft palate of complex mechanismsd.
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    Effects of periodontal basic therapy on pregnancy outcome and inflammatory cytokine of threatened abortion pregnant women
    Zhu Shanshan, Lu Zhiyue, Wang Shuang
    Inter J Stomatol. 2018, 45 (3):  319-323.  DOI: 10.7518/gjkq.2018.03.014
    Abstract ( 818 )   HTML ( 9 )   PDF(pc) (1134KB) ( 215 )   Save
    Objective To investigate the effects of periodontal basic therapy on pregnancy outcome and inflammatory cytokine of threatened abortion pregnant women. Methods Select 80 cases of pregnant women with threatened abortion symptoms as threatened abortion group and 86 cases of healthy pregnant women as normal pregnancy group. Select pregnant women with periodontitis from the above two groups, then randomly divided into treatment group and non-treatment group. For each treatment group the pregnant women were used periodontal treatment and regular maintenance, the non-treatment group accepted no special treatment except the oral hygiene instruction. Check the periodontal status [gingival sulcus bleeding index (SBI), probing depth (PD), and attachment loss (AL)] and the content of prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in serum in week 16 of pregnancy and before delivery, record the pregnancy outcomes of each pregnant women. Results In week 16 of pregnancy the SBI, PD and AL rate of threatened abortion group were higher than in normal pregnancy group, the difference was statistically significant. The improvement of SBI and PD before and after the periodontal treatment in threatened abortion and healthy pregnant women group have statistical significance. There was no significant difference in the content of PGE2, TNF-α and IL-6 before and after treatment in each group, there was no significant difference in the incidence of preterm low birth weight also. Conclusion Periodontal therapy is safe for pregnant women and can effectively improve the periodontal status, but cannot change the level of inflammatory factors in the blood and the pregnancy outcomes.
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    Reviews
    Preconception oral health care and genetic counseling
    Guan Shuyuan, Zhou Yuan, Zhou Xuedong, Zheng Liwei
    Inter J Stomatol. 2018, 45 (3):  324-330.  DOI: 10.7518/gjkq.2018.03.015
    Abstract ( 1135 )   HTML ( 52 )   PDF(pc) (1249KB) ( 833 )   Save
    Women preparing for pregnancy often overlook oral health examination. Oral diseases are not only relatively intractable, but may also have a serious impact on the mother and fetus. Therefore, ensuring the oral health of pregnant women is important. Many oral genetic diseases exhibit congenital maxillofacial deformities, thus seriously affecting the quality of life. Preconception genetic counseling can help people understand oral genetic diseases and assess the recurrence risk. This article explains the preconception and pregnancy oral health education, common methods of prevention and treatment of oral diseases, and genetic counseling of related content.
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    Research progress on the new attachment of apical periodontal ligament
    Ma Yanqun, Li Hong, Hou Benxiang
    Inter J Stomatol. 2018, 45 (3):  331-334.  DOI: 10.7518/gjkq.2018.03.016
    Abstract ( 852 )   HTML ( 7 )   PDF(pc) (1113KB) ( 495 )   Save
    Periapical diseases can induce apical periodontal ligament, cementum and alveolar bone destruction. The complete healing of periapical diseases includes the reestablishment of apical periodontal ligament and regeneration of cementum and alveolar bone. This review introduces the ways, mechanism and influence factors of new attachment of apical periodontal ligament after root canal therapy or apical surgery.
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    Correlation between periodontal diseases and Alzheimer’s disease
    Li Yihan, Pan Lanlan
    Inter J Stomatol. 2018, 45 (3):  335-339.  DOI: 10.7518/gjkq.2018.03.017
    Abstract ( 1333 )   HTML ( 23 )   PDF(pc) (1129KB) ( 592 )   Save
    Alzheimer’s disease (AD) is a chronic, progressive and irreversible neurodegenerative disease. The prevalence of AD increases exponentially with age. Although the pathophysiologic mechanism is not fully understood, inflammation has been confirmedto play a critical role in the pathogenesis of AD. Chronic periodontitisis a chronic immuno-inflammatory disease initiated by complex polymicrobial subgingival biofilm. Periodontal pathogens are implicated in the development of a number of inflammatory pathologies at remote organ sites, including brain tissue where AD occurs. The objective of this review is to present the relationship and possible mechanistic links between periodontal disease and AD.
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    Research progress on synthetic nanosilicates in bone tissue engineering
    Liang Xinyu, Shi Jiabo, Chen Wenchuan, Zhu Zhimin
    Inter J Stomatol. 2018, 45 (3):  340-345.  DOI: 10.7518/gjkq.2018.03.018
    Abstract ( 736 )   HTML ( 4 )   PDF(pc) (1146KB) ( 197 )   Save
    Synthetic nanosilicates are clay nanoparticles that have a cellular-level size, distinct charged and layered structure, and high surface-to-volume ratio, which enhances their interactions with proteins, cells, and polymers. Recently, researchers have focused on the implications of using synthetic nanosilicates in bone tissue engineering to understand their physicochemical and biological properties. The scope of this review includes the well-established use of synthetic nanosilicates for the adjustment of cell functions during bone tissue engineering. The interactions of synthetic nanosilicates with growth factors enable controlled delivery, and their mechanical or biological properties are enhanced when they interact matrix materials. This review is useful to future studies on synthetic nanosilicates.
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    Development of calcium phosphate scaffolds as drug delivery system in bone tissue engineering
    Zhang Yixin, Li Lei
    Inter J Stomatol. 2018, 45 (3):  346-350.  DOI: 10.7518/gjkq.2018.03.019
    Abstract ( 808 )   HTML ( 5 )   PDF(pc) (1123KB) ( 379 )   Save
    Calcium phosphates caffolds, especially hydroxyapatite and tricalcium phosphate, have recently attracted researchers’ attention for their use as drug delivery vehicledue to their excellent biocompatibility and bioactivity, which extends a new dimension to their applications. The development of calcium phosphate scaffolds prepared as drug delivery system in bone tissue engineering including its properties, release kinetics, and some of the current drug and growth factor delivery approaches were reviewed.
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    Research progress on nucleoside drugs against herpes virus
    Ji Ning, Zhao Hang, Zeng Xin, Chen Qianming
    Inter J Stomatol. 2018, 45 (3):  351-357.  DOI: 10.7518/gjkq.2018.03.020
    Abstract ( 798 )   HTML ( 9 )   PDF(pc) (1525KB) ( 268 )   Save
    Herpes virus can cause many diseases, such as keratitis, cold sores, herpes zoster, genital herpes, and encephalitis, etc. Aciclovir and related nucleoside analogues are gold standard molecules for treatment of herpetic diseases in clinical over the past decades. Here, the recent progress of anti-herpes virus nucleoside agents are summarized in this review, including structure characteristic, antiviral mechanism and clinical application.
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    Correlation between taste injury and diabetes
    Liu Caiyun, Tao Yiran
    Inter J Stomatol. 2018, 45 (3):  358-361.  DOI: 10.7518/gjkq.2018.03.021
    Abstract ( 4285 )   HTML ( 22 )   PDF(pc) (1168KB) ( 415 )   Save
    Taste is a very complex and important special feeling, and plays an important role in maintaining a stable environment within the animal. While diabetes affects the taste, taste impairment is one of the complications of diabetes. The occurrence of the sense of taste disorder in patients with diabetes significantly reduced their quality of life. In recent years, the prevalence of diabetes has become a rising trend. Therefore, it is very important to study the mechanism of the occurrence of diabetic taste damage . This paper reviews the research progress of diabetic taste damage.
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    Eruption prediction of dentigerous cyst-associated impacted teeth after marsupialization
    Yuan Yunyi, Duan Yufeng, Lei Yonghua
    Inter J Stomatol. 2018, 45 (3):  362-367.  DOI: 10.7518/gjkq.2018.03.022
    Abstract ( 1431 )   HTML ( 9 )   PDF(pc) (1138KB) ( 295 )   Save
    Adentigerous cyst is a common developmental odontogenic cyst, which affects dentomaxillofacial development of adolescents and interferes with tooth eruption. Marsupialization is widely used by orthodontists to treat dentigerous cysts, and it can benefit the natural eruption of a cyst-associated tooth. This article reviews relevant studies to provide the relative factors for predicting the eruption of cyst-associated teeth after marsupialization.
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    Research progress on diagnosis and treatment for submucous cleft palate
    Shi Jiahong, Lu Yong
    Inter J Stomatol. 2018, 45 (3):  368-372.  DOI: 10.7518/gjkq.2018.03.023
    Abstract ( 2088 )   HTML ( 19 )   PDF(pc) (1205KB) ( 595 )   Save
    Submucous cleft palate (SMCP) is a special type of cleft palate, presenting the classic clinical features which are a bifid uvula, a midline transluscent zone (zonapellucida) of the soft palate, and a palpable bony notch within the posterior hard palate. Abnormal insertion of the levator palatini muscle causes velopharyngeal insufficiency (VPI), otitis media and so on. There are always late discovery of SMCP and we observed that the VPI incidence after palatoplasty for SMCP patients was higher than that of other cases of cleft palate. So much attention should be paid to SMCP. This article makes a brief summary of the pathological causes, clinical manifestations, diagnosis and treatment.
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