Int J Stomatol

Inter J Stomatol ›› 2016, Vol. 43 ›› Issue (3): 298-302.doi: 10.7518/gjkq.2016.03.011

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Stem cells and scaffolds in dental pulp regeneration and revascularization

Li Zhou, Xu Qing’an   

  1. 1.The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China; 2. Dept. of Conservative Dentistry and Endodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079, China) This study was supported by the National Natural Science Foundation of China(81000435)and the Natural Science Foundation of Hubei Province(2014CFB722).
  • Received:2015-06-06 Revised:2016-02-29 Online:2016-05-01 Published:2016-05-01

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Abstract: Regenerative endodontics is defined as biologically based procedures designed to replace damaged structures, including dentin and root structures, as well as cells of the pulp–dentin complex. The methods of acquiring stem cells include stem-cell transplantation, cell homing, and induced bleeding. Stem-cell transplantation can yield ectopic dental pulp-like tissues, with the advantages of easily controlling the number of cells transplanted and selecting the optimal subpopulation of stem/progenitor cells. Meanwhile, cell homing is defined as the migration of endogenous host stem cells to the root canal of the offending teeth using signaling molecules. The process is also characterized by the subsequent proliferation and differentiation of the endogenous host stem cells into pulp–dentin-like tissues. Inducing pulp bleeding into the root canals of immature permanent teeth is an important step in regenerative endodontics. Scaffold refers to the structural support for the cells that synthesize tissues. This component promotes cell attachment and provides a specific environment conducive to pulp or dentin regeneration. Pulp regeneration cannot take place without revascularization or angiogenesis. Procedures such as infection control, pretreatment of root canals, and coronal sealing, provide a favorable environment for pulp regeneration, including pulp revascularization. The application and development of tissue engineering technology in endodontics bring new hope for dental pulp regeneration, despite the occurrence of numerous problems.

Key words: dental pulp regeneration, tissue en-gineering, stem cell, scaffold, growth factor, dental pulp regeneration, tissue en-gineering, stem cell, scaffold, growth factor

CLC Number: 

  • Q 81

TrendMD: 
[1] Murray PE, Garcia-Godoy F, Hargreaves KM. Regenerative endodontics: a review of current status and a call for action[J]. J Endod, 2007, 33(4):377-390.
[2] Egusa H, Sonoyama W, Nishimura M, et al. Stem cells in dentistry—part Ⅰ: stem cell sources[J]. J Prosthodont Res, 2012, 56(3):151-165.
[3] Hargreaves KM, Cohen S. Cohen’s pathways of the pulp[M]. 10th ed. St. Louis: Elsevier Medicine, 2011:602-619.
[4] 庞欣, 樊明文, 杨雪超. 组织工程牙髓再生的研究进展[J]. 口腔医学研究, 2013, 29(11):1086-1088.
Pang X, Fan MW, Yang XC. The research progress of dental pulp regeneration in tissue engineering[J]. J Oral Sci Res, 2013, 29(11):1086-1088.
[5] Ishizaka R, Iohara K, Murakami M, et al. Regeneration of dental pulp following pulpectomy by fractionated stem/progenitor cells from bone marrow and adipose tissue[J]. Biomaterials, 2012, 33(7):2109-2118.
[6] Iohara K, Imabayashi K, Ishizaka R, et al. Complete pulp regeneration after pulpectomy by transplantation of CD105+ stem cells with stromal cell-derived factor-1[J]. Tissue Eng Part A, 2011, 17(15/16):1911-1920.
[7] Huang GT, Yamaza T, Shea LD, et al. Stem/ progenitor cell-mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model[J]. Tissue Eng Part A, 2010, 16(2):605-615.
[8] Iohara K, Zheng L, Ito M, et al. Regeneration of dental pulp after pulpotomy by transplantation of CD31(-)/CD146(-) side population cells from a canine tooth[J]. Regen Med, 2009, 4(3):377-385.
[9] Kuo TF, Huang AT, Chang HH, et al. Regeneration of dentin-pulp complex with cementum and periodontal ligament formation using dental bud cells in gelatin-chondroitin-hyaluronan tri-copolymer scaffold in swine[J]. J Biomed Mater Res A, 2008, 86(4):1062-1068.
[10] Alongi DJ, Yamaza T, Song Y, et al. Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential[J]. Regen Med, 2010, 5(4):617-631.
[11] Mao JJ, Kim SG, Zhou J, et al. Regenerative endodontics: barriers and strategies for clinical translation [J]. Dent Clin North Am, 2012, 56(3):639-649.
[12] Lee CH, Cook JL, Mendelson A, et al. Regeneration of the articular surface of the rabbit synovial joint by cell homing: a proof of concept study[J]. Lancet, 2010, 376(9739):440-448.
[13] Kim K, Lee CH, Kim BK, et al. Anatomically shaped tooth and periodontal regeneration by cell homing[J]. J Dent Res, 2010, 89(8):842-847.
[14] Kim SG, Zhou J, Solomon C, et al. Effects of growth factors on dental stem/progenitor cells[J]. Dent Clin North Am, 2012, 56(3):563-575.
[15] Suzuki T, Lee CH, Chen M, et al. Induced migration of dental pulp stem cells for in vivo pulp regeneration[J]. J Dent Res, 2011, 90(8):1013-1018.
[16] Kim JY, Xin X, Moioli EK, et al. Regeneration of dental-pulp-like tissue by chemotaxis-induced cell homing[J]. Tissue Eng Part A, 2010, 16(10):3023-3031.
[17] Torabinejad M, Turman M. Revitalization of tooth with necrotic pulp and open apex by using plateletrich plasma: a case report[J]. J Endod, 2011, 37(2):265-268.
[18] Kim SG, Zheng Y, Zhou J, et al. Dentin and dental pulp regeneration by the patient’s endogenous cells [J]. Endod Topics, 2013, 28(1):106-117.
[19] Lovelace TW, Henry MA, Hargreaves KM, et al. Evaluation of the delivery of mesenchymal stem cells into the root canal space of necrotic immature teeth after clinical regenerative endodontic procedure[J]. J Endod, 2011, 37(2):133-138.
[20] Wang X, Thibodeau B, Trope M, et al. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis[J]. J Endod, 2010, 36(1):56-63.
[21] Wei X, Ling J, Wu L, et al. Expression of mineralization markers in dental pulp cells[J]. J Endod, 2007, 33(6):703-708.
[22] Galler KM, Eidt A, Schmalz G. Cell-free approaches for dental pulp tissue engineering[J]. J Endod, 2014, 40(4 Suppl):S41-S45.
[23] Chen FM, Wu LA, Zhang M, et al. Homing of endogenous stem/progenitor cells for in situ tissue regeneration: promises, strategies, and translational perspectives[J]. Biomaterials, 2011, 32(12):3189-3209.
[24] Ding RY, Cheung GS, Chen J, et al. Pulp revascularization of immature teeth with apical periodontitis: a clinical study[J]. J Endod, 2009, 35(5):745-749.
[25] Park M, Ahn BD. Immature permanent teeth with apical periodontitis and abscess treated by regenerative endodontic treatment using calcium hydroxide and MTA: a report of two cases[J]. Pediatr Dent, 2014, 36(3):107-110.
[26] Chueh LH, Ho YC, Kuo TC, et al. Regenerative endodontic treatment for necrotic immature permanent teeth[J]. J Endod, 2009, 35(2):160-164.
[27] Paryani K, Kim SG. Regenerative endodontic treatment of permanent teeth after completion of root development: a report of 2 cases[J]. J Endod, 2013, 39(7):929-934.
[28] Saoud TM, Sigurdsson A, Rosenberg PA, et al. Treatment of a large cystlike inflammatory periapical lesion associated with mature necrotic teeth using regenerative endodontic therapy[J]. J Endod, 2014, 40(12):2081-2086.
[29] Yuan Z, Nie H, Wang S, et al. Biomaterial selection for tooth regeneration[J]. Tissue Eng Part B Rev, 2011, 17(5):373-388.
[30] Kim HJ, Kim UJ, Leisk GG, et al. Bone regeneration on macroporous aqueous-derived silk 3-D scaffolds [J]. Macromol Biosci, 2007, 7(5):643-655.
[31] Zhang W, Walboomers XF, van Kuppevelt TH, et al. The performance of human dental pulp stem cells on different three-dimensional scaffold materials[J]. Biomaterials, 2006, 27(33):5658-5668.
[32] 李红玖, 姚志文, 王宾, 等. 牙髓再生的临床研究[J]. 临床口腔医学杂志, 2013, 29(6):366-368.
Li HJ, Yao ZW, Wang B, et al. The clinical studies of pulp regeneration in vivo[J]. J Clin Stomatol, 2013, 29(6):366-368.
（本文采编 王晴）
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[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 2005, 32(06): 458 -460 .
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[9] . [J]. Inter J Stomatol, 2008, 35(S1): .
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