Int J Stomatol

Int J Stomatol ›› 2021, Vol. 48 ›› Issue (6): 635-639.doi: 10.7518/gjkq.2021111

• Original Articles • Previous Articles     Next Articles

Role of transforming growth factor-β2 in promoting the proliferation and differentiation of dental pulp stem cells

Xiong Menglin1,2(),Wu Long1,2,Ma Li1,2,Zhao Jin1,2()   

  1. 1. Dept. of Cariology and Endodontics, The First Affiliated Hospital of Xinjiang Medical University（The Affiliated Stomatological Hospital of Xinjiang Medical University, Urumqi 830054, China
    2. Stomatological Disease Institute of Xinjiang Uyghur Autonomous Region, Urumqi 830054, China
  • Received:2021-02-05 Revised:2021-06-02 Online:2021-11-01 Published:2021-10-28
  • Contact: Jin Zhao E-mail:181099496@qq.com;merryljin@sina.com
  • Supported by:
    Natural Science Foundation of Xinjiang Uygur Autonomous Region(2020D01C253)

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Abstract:

Objective This work explores the effect of transforming growth factor-β2 (TGF-β2) on the proliferation and differentiation of dental pulp stem cells (DPSCs). Methods DPSCs were isolated, cultured, and randomly divided into negative control, positive control, and TGF-β2 groups. Tetramethylazoazole colorimetric method (MTT) was used to detect cell viability, and alkaline phosphatase (ALP) activity assay kit was applied to measure intracellular ALP activity. Western blot method was employed to detect Runt-related transcription factor 2 (Runx2), bone sialoprotein (BSP), and osteopontin (OPN) protein expression. Immunohistochemical staining was conducted to detect Runx2 positive expression. Results Compared with the positive control group, DPSCs activity continued to increase, intracellular ALP activity was up-regulated, Runx2, BSP, and OPN protein expression levels were increased, and Runx2 positive expression was increased after 3, 7, and 10 days of TGF-β2 treatment (P<0.05). Conclusion TGF-β2 can promote the proliferation and osteogenic differentiation of DPSCs.

Key words: transforming growth factor-β2, dental pulp stem cells, proliferation, differentiation

CLC Number: 

  • R781

TrendMD: 

Fig 1

Cell morphology of the fourth-generation DPSCs on day 3 inverted fluorescence microscope × 200"

Tab 1

Effect of TGF-β2 on the proliferation of DPSCs $\bar{x}±s$,n=3"

培养
时间/d		 OD490 F值 P值
阴性对照组 阳性对照组 TGF-β2组
1 0.31±0.02 0.30±0.06 0.38±0.03 0.951 0.438
3 0.47±0.02 0.65±0.03* 0.68±0.07*# 18.600 0.003
7 0.77±0.05 1.35±0.08* 1.75±0.08*# 144.050 <0.000 1
10 1.13±0.03 1.53±0.08* 1.82±0.06*# 103.170 <0.000 1

Tab 2

Effect of TGF-β2 on ALP activity of DPSCs x ?±s,n=3"

培养
时间/d		 OD520 F值 P值
阴性对照组 阳性对照组 TGF-β2组
1 10.23±1.29 9.59±0.33 10.50±0.76 0.84 0.476 9
3 12.78±0.51 15.30±1.78* 19.12±0.32*# 19.97 0.002 2
7 13.00±0.19 24.62±1.75* 38.17±2.69*# 138.12 <0.000 1
10 23.55±2.42 45.39±1.48* 54.12±3.60*# 106.22 <0.000 1

Fig 2

Runx2, BSP and OPN protein expression of DPSCs"

Tab 3

Effect of TGF-β2 on the expression of Runx2, BSP and OPN protein of DPSCs x ?±s,n=3"

检测
蛋白		 蛋白相对表达量 F值 P值
阴性对照组 阳性对照组 TGF-β2组
Runx2 0.18±0.02 0.54±0.03* 0.71±0.07*# 114.54 <0.0001
BSP 0.22±0.04 0.69±0.06* 0.92±0.08*# 105.55 <0.0001
OPN 0.18±0.03 0.37±0.04* 0.51±0.04*# 67.83 <0.0001

Fig 3

Runx2 expression of DPSCs immunohistochemical staining × 200"

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