Int J Stomatol

Int J Stomatol ›› 2021, Vol. 48 ›› Issue (4): 417-425.doi: 10.7518/gjkq.2021070

• Original Articles • Previous Articles     Next Articles

Experimental study on behavior changes of condylar chondrocytes in early stage of temporomandibular joint degeneration

Fang Lingli(),Tan Xi,Ye Yusi,Huang Lan,He Yao()   

  1. Stomatological Hospital of Chongqing Medical University & Chongqing Key Laboratory of Oral Diseases and Biomedical Science & Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2021-01-11 Revised:2021-04-06 Online:2021-07-01 Published:2021-06-30
  • Contact: Yao He E-mail:2018110874@stu.cqmu.edu.cn;yaohe@hospital.cqmu.edu.cn
  • Supported by:
    National Natural Science Foundation of China(31800786);General projects of China Postdoctoral fund(2018M640902)

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Abstract:

Objective To explore the biological behavioural changes in the proliferation, apoptosis and autophagy of condylar chondrocytes and changes in the related signal pathway in the early stage of cartilage degeneration under stress.Methods The forced mouth opening model was used, and samples were taken after 0 d (control group) and 10 d (experiment group) of inducing overloading force. After the laser confocal scanning of the condylar cartilage, 5-ethynyl-2’-deoxyuridine (EdU) staining was performed to detect cell proliferation. The expression of proliferation, apoptosis, autophagy and key proteins of the phosphatidylinositol 3-kinase/ protein kinase B (PI3K/Akt) pathway was detected by haematoxylin/eosin, toluidine blue and immunohistochemical stainings with paraffin sections of the intact temporomandibular joint. Results The thickness of the condylar cartilage increased, but the cell density and cartilage matrix secretion decreased in the experimental group. In addition, the number of proliferation and apoptosis positive cells and the expression of autophagy markers increased. At the same time, the PI3K/Akt pathway was also active. Conclusion The prolife-ration, apoptosis and autophagy activity of chondrocytes were activated to some extent in the early stage of cartilage degeneration induced by stress, with the activation of the PI3K/Akt pathway.

Key words: stress, condyle cartilage, degeneration, autophagy

CLC Number: 

  • R782.6

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Fig 1

Histomorphology of TMJ cartilage stimulated by stress"

Fig 2

Expression of TMJ cartilage matrix"

Fig 3

Proliferation and apoptosis of TMJ cartilage under stress stimulation"

Fig 4

Expression of autophagy in condylar chondrocytes under stress stimulation"

Fig 5

Expression of p-Akt and p-mTOR in stress-induced TMJ cartilage"

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