Int J Stomatol

Int J Stomatol ›› 2020, Vol. 47 ›› Issue (6): 627-634.doi: 10.7518/gjkq.2020091

• Original Articles • Previous Articles     Next Articles

Expression of autophagy related genes in mice periodontal tissue during orthodontic tooth movement

Yin Yuanyuan(),Ma Huayu,Li Xinyi,Xu Jingchen,Liu Ting,Chen Song,He Shushu()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-02-06 Revised:2020-07-09 Online:2020-11-01 Published:2020-11-06
  • Contact: Shushu He E-mail:1042520312@qq.com;heshushu-03@163.com
  • Supported by:
    National Natural Science Foundation of China(81671021)

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Abstract:

Objective To investigate the level of autophagy related genes in periodontal tissue during orthodontic tooth movement in an experimental C57B/6 mice model and explore the role of autophagy related genes in bone modeling during orthodontic tooth movement. Methods Thirty-11-week-old male C57B/6 mice were randomly divided into 3 groups. Each group contained 10 mice. The experimental model of orthodontic tooth movement was established in group A by applying active force-loading nickel-titanium springs. Springs were placed in group B without force loading. Group C served as blank group. All the springs were placed between the left maxillary first molar and incisor. The mice were sacrificed on day 12, and the left lateral maxillae were collected to prepare sample sections of the periodontal tissue of the left maxillary first molars. Tooth movement was measured via microcomputed tomography. Hematoxylin and eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) staining, and real-time quantitative polymerase chain reaction (RT-qPCR) were performed, and the expression levels of bone modeling-related genes [osteoprotegerin (OPG) and receptor activator of nuclear factor-κ B ligand (RANKL)] and autophagy related genes (including Atg5, Atg7 and Beclin-1) were detected. Results The mean distance of first molar tooth movement was 0.09 mm in group A. HE staining showed that the amount of periodontal space between the pressure side and tension side of the first molars differed in group A. TRAP staining showed the presence of TRAP-positive cells in the pressure side of the first molar, and the number of osteoclasts in the pressure side was higher than that in the tension side (P<0.05). RT-qPCR results indicated that compared with those in group B, the messenger RNA (mRNA) expression of OPG in the periodontal tissue of group A decreased, whereas the mRNA expression levels of RANKL, Atg5, Atg7 and Beclin-1 increased (P<0.05). Conclusion Autophagy related genes increased in periodontal tissues under orthodontic force. Autophagy may play an important role in bone modeling during orthodontic tooth movement by regulating osteoclastogenesis.

Key words: C57B/6 mice, orthodontic tooth movement, autophagy, messenger RNA, bone modeling

CLC Number: 

  • R783.5

TrendMD: 

Fig 1

Establishment of the tooth movement model"

Tab 1

Primers for PCR analysis of mice"

引物 序列
Mus GAPDH-F CGAGAATGGGAAGCTTGTCA
Mus GAPDH-R TTGGCTCCACCCTTCAAGT
Mus OPG-F GCACAGTGAGGAGGAAGACA
Mus OPG-R TGTGTTTCGCTCTGGGGTT
Mus RANKL-F ACACCTCACCATCAATGCTG
Mus RANKL-R TCCCGATGTTTCATGATGC
Mus Atg5-F GCCATCAACCGGAAACTC
Mus Atg5-R CAAGTGTGTGCAGCTGTCCA
Mus Atg7-F AAACCCCATGCTCCTCAACA
Mus Atg7-R GATCCAAGCTCACAGGTCCC
Mus Beclin-1-F ACGCTGTTTGGAGATCCTAG
Mus Beclin-1-R TTCGTCATCCAACTCCAGCT

Fig 2

MicroCT images of the first molars × 400"

Fig 3

Morphology of periodontal tissues of the first molars HE staining × 200"

Fig 4

Osteoclasts observation of the first molars TRAP staining × 200"

Fig 5

TRAP-positive osteoclasts number of the first molars"

Fig 6

Relative expression levels of OPG, RANKL, Atg5, Atg7 and Beclin-1 mRNA of the first molars"

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