Int J Stomatol

Int J Stomatol ›› 2020, Vol. 47 ›› Issue (6): 635-643.doi: 10.7518/gjkq.2020102

• Original Articles • Previous Articles     Next Articles

Physiological and biochemical study of acupoint mid-frequency pulse electrical stimulation on masseter muscle adapta-tions in rats with mandibular

Chen Qingqing(),Liu Zhenqiao,Wang Yurong()   

  1. Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chong-qing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2019-12-05 Revised:2020-06-10 Online:2020-11-01 Published:2020-11-06
  • Contact: Yurong Wang E-mail:2017110898@stu.cqmu.edu.cn;500007@hospital.cqmu.edu.cn
  • Supported by:
    Science and Technology Project of Traditional Chinese Medicine of Chongqing Health Com-mittee(2019ZY023110);Chongqing University Innovation Team Construction Plan(CXTDG201602006)

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Abstract:

Objective This study aimed to investigate the effects of acupoint mid-frequency pulse electrical stimulation on the surface electromyography and the expression of brain-derived neurotropic factor (BDNF) in the mandibular protrusion of Sprague-Dawley (SD) rats. Methods Sixty male SD rats were randomly divided into three large groups, namely, blank control, conditional control (with functional appliance and without acupoint mid-frequency pulse electrical stimulation therapy) and experimental groups (with functional appliance and acupoint mid-frequ- ency pulse electrical stimulation therapy). Each large group was randomly divided into four groups (3, 7, 14 and 21 days according to the number of experimental observation days), resulting in 12 groups (n=5). In this experiment, acupuncture points were selected on both sides of the rat, and the parame-ters of acupoint mid-frequency pulse electrical stimulation therapy were set as follows: sinusoidal waveform, 0-150 Hz modulation frequency, and 2 000-8 000 Hz intermediate frequency electrical frequency. The BL-420F biological signal acquisition and analysis system was used to record the electromyograms of rats in the resting state at different experimental periods (3, 7, 14, and 21 days). Immunohistochemistry and real-time quantitative polymerase chain reaction were carried out to detect the protein and mRNA expression levels of BDNF in the masseter muscle of rats. Results The electromyo-grams of masseter muscle, the protein and mRNA expression levels of BDNF first increased and then decreased in the experimental and condition control groups. Moreover, the electromyography of masseter muscle, the protein and mRNA expression levels of BDNF in the experimental group were higher than those in the condition control group on day 3 (P<0.05); the electromyography of masseter and the protein and mRNA expression levels of BDNF in the experimental group basically returned to the normal level on day 21, while those in the condition control group remained higher than those in the blank control group (P<0.05). Conclusion Acupoint mid-frequency pulse electrical stimulation therapy can improve the expression of BDNF in the masseter muscle of rats with functional mandibular protrusion in the early stage, accelerate the recovery of the masseter muscle to normal level, and may improve the treatment of functional appliance.

Key words: electric stimulation, mandibular protrusion, masseter muscle, electromyographic, brain-derived neuro-tropic factor

CLC Number: 

  • R78

TrendMD: 

Fig 1

Acupuncture point positioning"

Tab 1

The primer sequence for qRT-PCR"

引物名称 引物序列（5’—3’）
β-actin F：TCATGAAGTGTGACGTGGACATC
R：TGTTGCATTTGCGGGGACGATG
BDNF F：GAGCTGAGCGTGTGTGACAG
R：CGCCAGCCAATTCTCTTTTTGC

Fig 2

Resting electromyographic changes of the masseter muscle in the mandibular advancement in different groups"

Tab 2

Resting electromyographic of the masseter muscle in the mandibular advancement in different groups μV·ms-1, $\bar{x}\pm s$, n=5"

戴入时间 空白对照组 条件对照组 实验组
戴入前 143.81±5.20 147.71±4.27 150.61±2.41
戴入当时 149.40±5.83 214.71±4.79a 241.13±6.85ab
戴入3 d 142.43±7.65 202.67±5.89a 228.40±7.58ab
戴入7 d 149.99±2.13 188.16±3.92a 204.66±6.89ab
戴入14 d 144.44±5.46 178.74±3.65a 166.43±5.03ab
戴入21 d 148.59±3.15 175.49±2.65a 155.11±7.44b

Fig 3

Histological changes of masseter muscle in the mandibular advancement in different groups HE × 400"

Fig 4

Changes of expression of BDNF protein in masseter muscle in different groups SABC × 400"

Tab 3

Changes of the IOD of BDNF protein in masseter muscle $\bar{x}\pm s$, n=5"

戴入时间/d 空白对照组 条件对照组 实验组
3 158.16±15.64 167.50±5.96 183.69±16.88ab
7 156.49±12.22 225.20±10.54a 213.43±9.49a
14 150.53±6.94 229.14±9.68a 196.45±13.11ab
21 149.33±5.64 200.93±20.25a 155.63±10.09b

Tab 4

Relative expression of BDNF mRNA in masseter muscle in each group"

戴入时间/d 空白对照组 条件对照组 实验组
3 1.03±0.22 1.29±0.13 1.68±0.30ab
7 1.02±0.0.14 1.62±0.29a 1.43±0.20a
14 1.01±0.14 1.47±0.17a 1.36±0.34a
21 1.01±0.20 1.31±0.28a 1.17±0.33b
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