Int J Stomatol

Inter J Stomatol ›› 2015, Vol. 42 ›› Issue (3): 265-268.doi: 10.7518/gjkq.2015.03.005

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Feasibility study on tooth tissue engineering using cross-linked gelatin hydrogel prepared through photo-initiated polymerization

Li Lei1,2, Qiao Xiangchen2, Cui Caiyun1,2, Guo Weihua2,3, Tian Weidong1,2   

  1. 1. State Key Laboratory of Oral Diseases, Dept. of Traumatic and Plastic Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2. National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China; 3. State Key Laboratory of Oral Diseases, Dept. of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2014-10-12 Revised:2015-01-12 Online:2015-05-01 Published:2015-05-01

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Abstract:

Objective The objective of this study is to determine the characteristics, physicochemical property, and bio-compatibility of gelatin methacrylate(GM) hydrogel, with an aim to introduce this as a new scaffold compound for dental pulp regeneration. Methods Gelatin(G) was cross-linked with methylacrylic acid(MA) to produce GM. Its surface appearance was assessed using scanning electron microscope(SEM), and its characteristics were tested using nuclear magnetic resonance(NMR). Hydrogel is formed by adding Irgacure2959 into the GM solution. The mixture was subsequently exposedto ultraviolet ray(UV). Rheometer was used to test viscoelastity, which reflects the mechanical property of GM. The effects of GM to the proliferation of human dental pulp cells(hDPCs) were revealed by Trypan Blue and cell counting kit-8(CCK-8). Results GM hydrogel, which was formed from cross-linking of gelatin and methylacrylic acid, not only have been improved in its mechanical property and porosity, but also supported the growth of hDPCs. Conclusion GM hydrogel is a potential scaffold material in dental pulp regeneration research.

Key words: cross-linked gelatin, photo-initiated polymerization, dental pulp cell, scaffold, tissue engineering

CLC Number: 

  • R 782

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