国际口腔医学杂志 ›› 2021, Vol. 48 ›› Issue (4): 417-425.doi: 10.7518/gjkq.2021070
Fang Lingli(),Tan Xi,Ye Yusi,Huang Lan,He Yao(
)
摘要:
目的 探索应力诱导颞下颌关节(TMJ)退行性变早期,髁突软骨细胞增殖、凋亡和自噬行为表现及相关信号通路的变化。方法 使用小鼠强制张口模型分别对小鼠加力0 d(对照组)和10 d(实验组),加力结束后取样。髁突软骨行激光共聚焦扫描,5-乙炔基-2’脱氧尿嘧啶核苷(EdU)染色检测增殖;完整关节区切片行苏木精/伊红、甲苯胺蓝以及免疫组化染色检测增殖、凋亡、自噬及磷脂酰肌醇3-激酶/丝氨酸/苏氨酸蛋白激酶(PI3K/Akt)通路关键蛋白的表达。结果 实验组小鼠与对照组小鼠相比,髁突软骨增厚,细胞密度降低,基质分泌减少;且增殖和凋亡阳性细胞增多,自噬活性增加;伴随PI3K/Akt通路活化。结论 应力诱导TMJ退行性变早期,软骨细胞在增殖、凋亡及自噬活性上均有一定程度的激活,并伴随了PI3K/Akt通路活化。
中图分类号:
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