国际口腔医学杂志

国际口腔医学杂志 ›› 2020, Vol. 47 ›› Issue (2): 159-165.doi: 10.7518/gjkq.2020039

• 论著 • 上一篇    下一篇

口腔鳞状细胞癌中转化因子2β的表达及潜在的临床意义

郝福1,宁毅1,孙睿2(),郑晓旭3   

  1. 1. 山西医科大学口腔医学院·口腔医院 太原 030001
    2. 山西省人民医院口腔颌面外科 太原 030012
    3. 马里兰大学医学院附属医院肾内科 巴尔的摩 21201
  • 收稿日期:2019-07-05 修回日期:2019-12-10 出版日期:2020-03-01 发布日期:2020-03-12
  • 通讯作者: 孙睿 E-mail:ruidoctor@163.com
  • 作者简介:郝福,住院医师,硕士,Email： 283425020@qq.com
  • 基金资助:
    山西省自然科学基金(2011011037-2)

Expression and potential clinical significance of Transformer 2β in oral squamous cell carcinoma

Hao Fu1,Ning Yi1,Sun Rui2(),Zheng Xiaoxu3   

  1. 1. Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, China
    2. Dept. of Oral and Maxillofacial Surgery, Shanxi Provincial People’s Hospital, Taiyuan 030012, China
    3. Dept. of Nephrology, Affiliated Hospital of Medical College, University of Maryland, Baltimore 21201, USA
  • Received:2019-07-05 Revised:2019-12-10 Online:2020-03-01 Published:2020-03-12
  • Contact: Rui Sun E-mail:ruidoctor@163.com
  • Supported by:
    This study was supported by Program for Natural Science Foundation of Shanxi Province(2011011037-2)

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摘要：

目的 探讨口腔鳞状细胞癌（OSCC）中转化因子2β（Tra2β）的表达及临床意义。方法 利用免疫组化检测64例OSCC中Tra2β蛋白的表达,并分析Tra2β蛋白表达与临床病理参数间的关系。Western blot和实时荧光聚合酶链反应（RT-PCT）检测22例OSCC肿瘤和癌旁组织中Tra2β蛋白和mRNA的表达情况。Kaplan-Meier分析Tra2β蛋白的表达对OSCC患者生存率的影响。Cox多因素生存分析Tra2β蛋白表达对OSCC患者预后的影响。结果 免疫组化检测结果表明Tra2β蛋白在OSCC中表达,并且它的阳性表达与分化程度和临床分期有关。实验结果表明,Tra2β蛋白和mRNA在OSCC中的表达趋势一致,肿瘤组织中Tra2β蛋白和mRNA的表达均高于癌旁组织。Kaplan-Meier分析表明,Tra2β蛋白高表达患者的预后较差。Cox多因素分析表明,Tra2β蛋白是OSCC患者预后评估的一个指标。结论 Tra2β可能为OSCC治疗的一个新的分子靶点。

关键词: 转化因子2β, 口腔鳞状细胞癌, 预后

Abstract:

Objective This study aimed to investigate the expression and clinical significance of Transformer 2β (Tra2β) in oral squamous cell carcinoma (OSCC). Methods Sixty-four samples of OSCC were subjected to immunohistochemistry to analyze the expression of Tra2β protein in OSCC and to explore the relationship between the expression of Tra2β protein and different clinical pathological parameters. Twenty-two pairs of fresh OSCC tissues and adjacent tissues were used to explore the expression of Tra2β mRNA and protein through real-time polymerase chain reaction and Western blot analyses. The Kaplan-Meier method was used to estimate and validate the effect of Tra2β protein on the survival rate of patients with OSCC. Cox multivariate regression was used to analyze the effect of Tra2β protein on the prognosis of patients with OSCC. Results Immunohistochemistry results revealed that Tra2β protein was expressed in OSCC, and the positive expression of Tra2β protein was significantly associated with the differentiation degree and clinical stage of OSCC. Moreover, the expression trends of Tra2β protein and mRNA were consistent, and the expression levels of Tra2β protein and mRNA in OSCC tissues were higher than those in adjacent tissues. Kaplan-Meier survival analysis showed that patients with OSCC and Tra2β protein expression had poor prognoses. Cox multivariate regression analysis demonstrated that the Tra2β protein was an independent prognostic factor of OSCC. Conclusion Tra2β may be a new molecular target for the treatment of OSCC.

Key words: Transformer 2β, oral squamous cell carcinoma, prognosis

中图分类号: 

  • R739.81

表 1

RT-PCR引物序列及产物长度"

基因 引物（5’~3’） 产物长度/bp
Tra2β 正向引物：TGCGGAGGCGGTGCGGAGCATT;反向引物：CATGACTCCTGGCTGCTGTCGCC 149
GADPH 正向引物：CAACAGCCTCAAGATCATCAGC;反向引物：CATGAGTCCTTCCACGATACCA 103
临床病理参数 阴性（-） 弱阳性（+） 中阳性（++） 强阳性（+++） P值
年龄/岁 >60 3 18 13 2 0.341
≤60 0 15 9 4
性别 男 1 16 10 5 0.443
女 2 17 12 1
淋巴结转移 是 0 3 7 1 0.162
否 3 30 15 5
病变部位 唇 1 10 5 0 0.235
舌 0 2 3 1
颊 0 3 4 1
牙龈 1 17 7 2
腭 1 1 3 2
临床分期 T1 1 18 5 2 <0.001*
T2 1 6 6 2
T3 1 6 6 0
T4 0 3 5 2
分化程度 高分化 1 26 3 1 0.046*
中分化 1 6 18 3
低分化 1 1 1 2

图 1

Tra2β蛋白在OSCC中表达的免疫组化染色 A：阴性对照 × 100;B：弱阳性表达 × 200;C：中阳性表达 × 200;D：强阳性表达 × 200。"

表 2

Tra2β蛋白表达与64例OSCC临床病理参数的关系"

图 2

Tra2β蛋白在OSCC肿瘤和癌旁组织中的表达 A：Tra2β蛋白在8例OSCC患者肿瘤和癌旁组织中的表达;B：Tra2β蛋白在Hela细胞中的表达;C：Tra2β蛋白在22例OSCC肿瘤和癌旁组织中的相对表达量。*示P<0.05,差异有统计学意义。"

图 3

Tra2β mRNA在22例OSCC肿瘤和癌旁组织中的相对表达量 注：*示P<0.05,差异有统计学意义。"

图 4

Tra2β蛋白表达对OSCC患者生存率的影响"

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