Int J Stomatol

Inter J Stomatol ›› 2012, Vol. 39 ›› Issue (3): 308-311.doi: 10.3969/j.issn.1673-5749.2012.03.008

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Effects and mechanism of daidzein on osteodifferentiation of stem cells derived from human exfoliated deciduous teeth

Wu Bin1, Xie Chun1, Huang Qian2, Du Xinya1, Huang Jian1, Meng Qiuju1, You Yuehua1.   

  1. 1. Center of Stomatology, Baoan Longhua Stomatological Hospital of Shenzhen, Shenzhen 518109, China; 2. Research Institute of Plastic Surgery, Guangzhou Medical College, Zhanjiang 524000, China
  • Received:2011-11-11 Revised:2012-02-27 Online:2012-05-01 Published:2012-05-01

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Abstract:

Objective To investigate the effects and mechanism of daidzein on osteodifferentiation of stem cells derived from human exfoliated deciduous teeth（SHED）. Methods The 3rd passage SHED were incubated with 1, 10, 100 μmol·L－1 daidzein and normal culture medium（control）. Then alkaline phosphatase（AKP） activities were examined at 3, 6 and 9 d, and osteocalcin（OC） in each group was detected at 7, 14 and 21 d. In addition, corebinding factor（cbf）-α1 mRNA expression were detected by RT-PCR at 3, 6 and 9 d after culture. Results 3 d later, AKP activities in SHED were enhanced by 10 μmol·L-1 daidzein（vs. control, P<0.05）, all of three concentrations（ 1, 10, 100 μmol·L-1） daidzein improved AKP activities significantly at 6 and 9 d（vs. control, P<0.001）. Medium and high dose（10, 100 μmol·L-1） daidzein could enhance OC expression in SHED at 14 d after induced culture（vs. control, P<0.001）, and all of the doses daidzein increased OC expression at 21 d（vs. control, P<0.001）. Additionally, RT -PCR showed that 10 and 100 μmol·L -1 daidzein could significantly increase cbf -α1 mRNA expression at the 3rd, while 1 μmol·L－1 daidzein only slightly increase cbf-α1 mRNA expression; at the 6th and 9th d, however, three concentration daidzein increased cbf-α1 mRNA expression obviously. Conclusion Daidzein could stimulate osteodifferentiation of SHED in vitro, and the possible mechanism is its role of promoting the expression of cbf-α1 gene.

Key words: daidzein, stem cell derived from human exfoliated deciduous teeth, osteoblast differentiation, mechanism


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