国际口腔医学杂志

国际口腔医学杂志 ›› 2020, Vol. 47 ›› Issue (2): 138-145.doi: 10.7518/gjkq.2020018

• 牙周专栏 • 上一篇    下一篇

非编码RNA调控人牙周膜干细胞成骨向分化的研究进展

王润婷1,2,房付春1,2()   

  1. 1. 南方医科大学南方医院口腔科 广州 510515
    2. 南方医科大学口腔医学院 广州 510515
  • 收稿日期:2019-06-14 修回日期:2019-10-23 出版日期:2020-03-01 发布日期:2020-03-12
  • 通讯作者: 房付春
  • 作者简介:王润婷,医师,学士,Email： wrunting@163.com
  • 基金资助:
    国家自然科学基金(81600882)

Progress in research of non-coding RNAs in osteogenic differentiation of human periodontal ligament stem cells

Wang Runting1,2,Fang Fuchun1,2()   

  1. 1. Dept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    2. School of Stomatology, Southern Medical University, Guangzhou 510515, China
  • Received:2019-06-14 Revised:2019-10-23 Online:2020-03-01 Published:2020-03-12
  • Contact: Fuchun Fang
  • Supported by:
    This study was supported by National Natural Science Foundation of China(81600882)

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摘要：

牙周炎是一种常见的炎症性疾病,以牙齿支持结构的进行性损伤为特点,是我国成人牙齿丧失的主要原因。治疗牙周炎的目的不仅是通过控制炎症来阻止疾病发展,更重要的是获得牙周再生。人牙周膜干细胞具有成骨向分化潜能,有望在牙周组织修复再生的临床应用上发挥重要作用。非编码RNA（ncRNA）一般是指不编码蛋白质的RNA。伴随高通量检测技术的不断发展,发现了大量的种类丰富的ncRNA,其生物学功能也被不断揭示。越来越多证据显示,ncRNA在分子机制及细胞组织层面对疾病的发生、发展起重要调控作用,因此探索ncRNA调控机制可为牙周再生的研究提供新思路。本综述主要阐述了目前研究较多的几种ncRNA在人牙周膜干细胞成骨向分化中的调控机制。

关键词: 非编码RNA, 牙周再生, 牙周膜干细胞, 成骨向分化

Abstract:

Periodontitis is a common inflammatory disease that is characterised by progressive damage to dental supporting structure. Periodontitis is the main cause of tooth loss in adults in China. The aim of periodontal treatment is not only to control inflammation and prevent disease development but also to obtain periodontal regeneration. Human periodontal ligament stem cells (PDLSCs), which are capable of osteogenic differentiation, are expected to play important roles in clinical application of periodontal tissue repair and regeneration. Non-coding RNAs (ncRNAs) generally refer to RNAs that do not encode proteins. The continuous development of high-throughput detection techniques has allowed the identification of a large number of ncRNAs and their biological functions. Increasing numbers of ncRNAs have been reported to function in the pathogenesis of diseases at molecular and cellular levels. Therefore, research on ncRNAs can provide new insights into periodontal regeneration. This review focuses on the regulatory mechanism of several ncRNAs in the osteogenic differentiation of human PDLSCs.

Key words: non-coding RNA, periodontal regeneration, periodontal ligament stem cell, osteogenic differentiation

中图分类号: 

  • Q756

表 1

ncRNA参与牙周膜干细胞成骨分化调控"

名称 类型 诱导方式 调控水平 调控作用 可能的靶基因或作用通路 文献
miR-24-3p miRNA 矿化 转录后水平 抑制 靶向作用Smad5 [15]
miR-21 miRNA 矿化 转录后水平 抑制 靶向作用Smad5 [16]
矿化 转录后水平 抑制 miR-21/Spry1调控轴介导TNF-α对牙周膜干细胞的抑制成骨 [17]
作用
剪切力 转录后水平 抑制 靶向作用ACVR2B [29]
miR-203 miRNA 矿化 转录后水平 抑制 靶向作用RUNX2 [18]
miR-1305 miRNA 矿化 转录后水平 抑制 靶向作用RUNX2 [19]
miR-218 miRNA 矿化 转录后水平 抑制 靶向作用RUNX2 [20]
miR-214 miRNA 矿化 转录后水平 抑制 靶向作用ATF4 [21]
矿化 转录后水平 抑制 通过靶向作用β-连环蛋白1调节Wnt/β-连环蛋白信号通路 [22]
miR-17 miRNA 矿化 转录后水平 抑制 通过靶向作用TCF3调节经典Wnt信号通路 [23-24]
miR-31 miRNA 矿化 转录后水平 抑制 靶向作用Satb2 [25]
miR-200c miRNA 矿化 转录后水平 促进 抑制IL-6、IL-8和CCL-5 [26]
miR-543 miRNA 矿化 转录后水平 促进 靶向作用TOB2 [27]
miR-22 miRNA 矿化 转录后水平 促进 抑制HDAC6 [28]
名称 类型 诱导方式 调控水平 调控作用 可能的靶基因或作用通路 文献
MEG3 lncRNA 矿化 转录水平 抑制 与hnRNPK结合,影响BMP2转录 [37]
ANCR lncRNA 矿化 转录水平 抑制 通过调控GSK3β的表达抑制Wnt/β-连环蛋白信号通路,下调 [38]
RUNX2
矿化 转录后水平 抑制 ANCR/miR-758/Notch2 [40]
TUG1 lncRNA 矿化 转录水平 促进 Lin28A蛋白作为TUG1的RBP形成正性调控网络 [36]
PCAT1 lncRNA 矿化 转录后水平 促进 PCAT1/miR-106a-5p/BMP2 PCAT1/miR-106a-5p/E2F5前馈调 [41]
节网络
HIF1A-AS2 lncRNA 缺氧 转录后水平 促进 通过碱基配对与HIF-1α形成双链RNA,提高蛋白质翻译水平 [42]
CDR1 circRNA 矿化 转录后水平 促进 竞争性结合miR-7,使GDF5上调、Smad1/5/8及p38MAPK [46]
磷酸化
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