Int J Stomatol

Inter J Stomatol ›› 2015, Vol. 42 ›› Issue (4): 415-419.doi: 10.7518/gjkq.2015.04.010

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Effects of high glucose on osteogenic differentiation of periodontal ligament cells in vitro

Xia Jiajia1, Wang Lan2, Zhang Yanzhen3.   

  1. 1. Dept. of General Dentistry, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China; 2. Dept. of Conservative Dentistry and Endodontics, The Affiliated Hospital of Stomatology, Chongqing Medcial University, Chongqing Research Center for Oral Diseases and Biomedical Sciences, Chongqing 401147, China; 3. Dept. of Orthodontics, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China
  • Online:2015-07-01 Published:2015-07-01

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Abstract:

Objective To isolate the periodontal ligament cells(PDLCs) and study the effect of high glucose concentration levels on the cellular activity and osteogenic differentiation of PDLCs from non-diabetic patients in vitro. Methods PDLCs were cultured for 14 days in 0 mg•L-1 glucose medium(C group), 1 100 mg•L-1 glucose medium(L group), and 4 500 mg•L-1 glucose medium(H group). Methyl thiazolyl tetrazolium(MTT) assay for cellular viability was performed. The osteogenic differentiation capacity of PDLCs was evaluated by alizarin red staining and alkaline phosphatase(ALP) activity assaying and real time-transcription polymerase chain reaction. Results H group significantly inhibited the proliferation of PDLCs and reduced the optic density of the MTT and ALP activity assay. The difference was significant(P<0.05). Concerning the mineralized nodule formation, the percentage of the calcified area and the expression of osteogenic genes(ALP, Runx-2, Col-Ⅰ) to the total culture dish of PDLCs in the H group were significantly lower than that in the L and C groups(P<0.05). Conclusion High glucose inhibits the proliferation and differentiation of PDLCs.

Key words: glucose, periodontal ligament cell, osteogenic differentiation


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