Int J Stomatol

Int J Stomatol ›› 2025, Vol. 52 ›› Issue (3): 358-365.doi: 10.7518/gjkq.2025045

• Original Articles • Previous Articles    

Expression and function of branch-chain amino acid transaminase 1 in oral squamous cell carcinoma

Yijie Li1(),Zhenying Yuan1,Ming Li2()   

  1. 1.Dept. of Dental Aesthetics, Changsha Stomatological Hospital, Changsha 410000, China
    2.Xiangya School/Hospital of Stomatology, Central South University, Key Laboratory of Oral Health Research in Hunan Province, Changsha 410000, China
  • Received:2024-04-25 Revised:2024-10-29 Online:2025-05-01 Published:2025-04-30
  • Contact: Ming Li E-mail:327623445@qq.com;limingq248@163.com
  • Supported by:
    Key Research and Development Project of Hunan Province(2022SK2052)

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Abstract:

Objective This study aimed to analyze the expression and biological function of branch-chain amino acid transaminase 1 (BCAT1) in oral squamous cell carcinoma (OSCC). Methods The expression of BCAT1 in head and neck squamous cell carcinoma (HNSC) patients and its relationship with prognosis were analyzed by bioinformatics. To detect BCAT1 gene expression in tumor and adjacent tissues, we selected patients with OSCC diagnosed in the Department of Oral and Maxillofacial Surgery, Xiangya Stomatology Hospital, Central South University from 2021 to 2022. BCAT1 gene expression in human OSCC cell line and normal oral keratinocytes was analyzed. After plasmid overexpression or knockdown of BCAT1 gene, the proliferation, migration, and invasion ability of human OSCC cells were detected by cell proliferation, wound-healing, and invasion assays, respectively. Results BCAT1 was highly expressed in cancer tissues of HNSC patients in the Cancer Genome Atlas (TCGA) database and was one of the independent prognostic factors and potential diagnostic markers of patients. Results of cellular assays showed that BCAT1 expression was upregula-ted in human OSCC tissues or cell lines, thereby regulating the proliferation, migration, and invasion of OSCC cells. Conclusion BCAT1 gene is a potential therapeutic target for human OSCC.

Key words: branch-chain amino acid transaminase 1, oral squamous cell carcinoma, head and neck squamous cell carcinoma, proliferation, migration, invasion

CLC Number: 

  • R739.8

TrendMD: 

Fig 1

BCAT1 gene was highly expressed in HNSC, and was associated with poor survival prognosis in HNSC patients"

Fig 2

The expression of BCAT1 gene and protein were up-regulated in OSCC tissues"

Fig 3

BCAT1 gene and protein were highly expressed in OSCC cell lines"

Fig 4

Effects of BCAT1 knockdown on proliferation, migration and invasion of SCC9 cells"

Fig 5

Effects of BCAT1 overexpression on proliferation, migration and invasion of CAL27 cells"

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