Int J Stomatol

Int J Stomatol ›› 2026, Vol. 53 ›› Issue (6): 925-935.doi: 10.7518/gjkq.2026605

• Review • Previous Articles    

Application of antimicrobial peptide in the surface modification of implants

Zhewei Li(),Boxin Zhang,Mengyuan Zhang,Ping Sun()   

  1. Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310000, China
  • Received:2025-04-11 Revised:2026-01-21 Online:2026-11-01 Published:2026-10-29
  • Contact: Ping Sun E-mail:22418934@zju.edu.cn;nosleep@zju.edu.cn
  • Supported by:
    Interdisciplinary Research Project of Stomatology Hospital Affiliated to Zhejiang University School of Medicine(RD2022JCXK01);Public Welfare Technology Project of Science Technology Department of Zhejiang Province(LGF22H-140007)

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Abstract:

This study explores the application of antimicrobial peptide (AMP) in implant surface modification to address implant failure caused by peri-implantitis. By analyzing the complex antibacterial mechanisms of AMP, including membrane targeting, metabolic disruption, protein synthesis inhibition, and immunomodulation, this study systematically reviews the research progress in three surface modification strategies: direct coating, alkylated coating, and dynamic release coating. Results indicate that AMP effectively inhibited bacterial biofilm formation, reduced the incidence of peri-implantitis, and enhanced the antimicrobial properties and biocompatibility of implants, thereby promoting osseointegration. AMP shows promising potential in implant surface modification. Future research should focus on optimizing AMP structures and functions, as well as strengthening clinical validation, to improve the success rate of dental implants and patient outcomes.

Key words: implant, surface modification, antimicrobial peptide, peri-implantitis, antibacterial mechanism

CLC Number: 

  • R783.4

TrendMD: 

Fig 1

Schematic diagram of four action mechanisms of membrane-active antimicrobial peptides"

Fig 2

Schematic diagram of three coating strategies"

[1] Thanissorn C, Guo J, Jing Ying Chan D, et al. Success rates and complications associated with single immediate implants: a systematic review[J]. Dent J (Basel), 2022, 10(2): 31.
[2] Rosales-Leal JI, Rodríguez-Valverde MA, Mazzaglia G, et al. Effect of roughness, wettability and morphology of engineered titanium surfaces on osteoblast-like cell adhesion[J]. Colloids Surf A Physicochem Eng Asp, 2010, 365(1/2/3): 222-229.
[3] Nguyen-Hieu T, Borghetti A, Aboudharam G. Peri-implantitis: from diagnosis to therapeutics[J]. J Investig Clin Dent, 2012, 3(2): 79-94.
[4] Wu SY, Xu JM, Zou LY, et al. Long-lasting renewable antibacterial porous polymeric coatings enable titanium biomaterials to prevent and treat peri-implant infection[J]. Nat Commun, 2021, 12: 3303.
[5] Shemtov-Yona K, Rittel D. Fatigue of dental implants: facts and fallacies[J]. Dent J (Basel), 2016, 4(2): 16.
[6] Liu ZQ, Liu XL, Ramakrishna S. Surface enginee-ring of biomaterials in orthopedic and dental implants: strategies to improve osteointegration, bacteriostatic and bactericidal activities[J]. Biotechnol J, 2021, 16(7): e2000116.
[7] Chun Giok K, Menon RK. The microbiome of peri-implantitis: a systematic review of next-generation sequencing studies[J]. Antibiotics, 2023, 12(11): 1610.
[8] Ghensi P, Manghi P, Zolfo M, et al. Strong oral plaque microbiome signatures for dental implant di-seases identified by strain-resolution metagenomics[J]. NPJ Biofilms Microbiomes, 2020, 6(1): 47.
[9] Jenkinson HF, Lamont RJ. Oral microbial communities in sickness and in health[J]. Trends Microbiol, 2005, 13(12): 589-595.
[10] Gristina AG, Naylor P, Myrvik Q. Infections from biomaterials and implants: a race for the surface[J]. Med Prog Technol, 1988, 14(3/4): 205-224.
[11] Batoni G, Maisetta G, Esin S. Antimicrobial pepti-des and their interaction with biofilms of medically relevant bacteria[J]. Biochim Biophys Acta, 2016, 1858(5): 1044-1060.
[12] Schwarz F, Derks J, Monje A, et al. Peri-implantitis[J]. J Periodontol, 2018, 89(): S267-S290.
[13] Fang DD, Yuran S, Reches M, et al. A peptide coa-ting preventing the attachment of Porphyromonas gingivalis on the surfaces of dental implants[J]. J Periodontal Res, 2020, 55(4): 503-510.
[14] Nagay BE, Dini C, Cordeiro JM, et al. Visible-light-induced photocatalytic and antibacterial activity of TiO2 codoped with nitrogen and bismuth: new perspectives to control implant-biofilm-related disea-ses[J]. ACS Appl Mater Interfaces, 2019, 11(20): 18186-18202.
[15] Schwarz F, Derks J, Monje A, et al. Peri-implantitis[J]. J Clin Periodontol, 2018, 45(): S246-S266.
[16] Hizal F, Zhuk I, Sukhishvili S, et al. Impact of 3D hierarchical nanostructures on the antibacterial efficacy of a bacteria-triggered self-defensive antibiotic coating[J]. ACS Appl Mater Interfaces, 2015, 7(36): 20304-20313.
[17] Mahlapuu M, Håkansson J, Ringstad L, et al. Antimicrobial peptides: an emerging category of therapeutic agents[J]. Front Cell Infect Microbiol, 2016, 6: 194.
[18] Bin Hafeez A, Jiang XK, Bergen PJ, et al. Antimicrobial peptides: an update on classifications and databases[J]. Int J Mol Sci, 2021, 22(21): 11691.
[19] Riool M, de Breij A, Drijfhout JW, et al. Antimicrobial peptides in biomedical device manufacturing[J]. Front Chem, 2017, 5: 63.
[20] Tan XW, Goh TW, Saraswathi P, et al. Effectiveness of antimicrobial peptide immobilization for preven-ting perioperative cornea implant-associated bacte-rial infection[J]. Antimicrob Agents Chemother, 2014, 58(9): 5229-5238.
[21] Epand RM, Walker C, Epand RF, et al. Molecular mechanisms of membrane targeting antibiotics[J]. Biochim Biophys Acta, 2016, 1858(5): 980-987.
[22] Raheem N, Straus SK. Mechanisms of action for antimicrobial peptides with antibacterial and antibiofilm functions[J]. Front Microbiol, 2019, 10: 2866.
[23] Luo Y, Song YZ. Mechanism of antimicrobial peptides: antimicrobial, anti-inflammatory and antibiofilm activities[J]. Int J Mol Sci, 2021, 22(21): 11401.
[24] Kumar P, Kizhakkedathu JN, Straus SK. Antimicrobial peptides: diversity, mechanism of action and strategies to improve the activity and biocompatibility in vivo [J]. Biomolecules, 2018, 8(1): 4.
[25] Ahmed TAE, Hammami R. Recent insights into structure-function relationships of antimicrobial peptides[J]. J Food Biochem, 2019, 43(1): e12546.
[26] He Q, Yang ZH, Zou ZP, et al. Combating Escherichia coli O157: H7 with functionalized chickpea-derived antimicrobial peptides[J]. Adv Sci, 2023, 10(6): e2205301.
[27] Wu RX, Li N, Huang WL, et al. Melittin suppresses ovarian cancer growth by regulating SREBP1-me-diated lipid metabolism[J]. Phytomedicine, 2025, 137: 156367.
[28] Sass V, Schneider T, Wilmes M, et al. Human β-defensin 3 inhibits cell wall biosynthesis in staphylococci[J]. Infect Immun, 2010, 78(6): 2793-2800.
[29] de Leeuw E, Li CQ, Zeng PY, et al. Functional interaction of human neutrophil peptide-1 with the cell wall precursor lipid Ⅱ[J]. FEBS Lett, 2010, 584(8): 1543-1548.
[30] Le CF, Fang CM, Sekaran SD. Intracellular targe-ting mechanisms by antimicrobial peptides[J]. Antimicrob Agents Chemother, 2017, 61(4): e02340-16.
[31] Wang DS, Yu XJ, Sheng P, et al. The transcriptomic mechanism of a novel autolysis induced by a recombinant antibacterial peptide from chicken expressed in Pichia pastoris[J]. Molecules, 2022, 27(6): 2029.
[32] Wang N, Gao J, Wang QY, et al. Antimicrobial peptide antibiotics inhibit aerobic denitrification via affecting electron transportation and remolding carbon metabolism[J]. J Hazard Mater, 2022, 431: 128616.
[33] Ghosh A, Kar RK, Jana J, et al. Indolicidin targets duplex DNA: structural and mechanistic insight through a combination of spectroscopy and microscopy[J]. Chem Med Chem, 2014, 9(9): 2052-2058.
[34] Marchand C, Krajewski K, Lee HF, et al. Covalent binding of the natural antimicrobial peptide indoli-cidin to DNA abasic sites[J]. Nucleic Acids Res, 2006, 34(18): 5157-5165.
[35] Huang CK, Zhan LJ, Ai YX, et al. LSP1 is the major substrate for mitogen-activated protein kinase-activated protein kinase 2 in human neutrophils[J]. J Biol Chem, 1997, 272(1): 17-19.
[36] Biragyn A, Surenhu M, Yang D, et al. Mediators of innate immunity that target immature, but not mature, dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens[J]. J Immunol, 2001, 167(11): 6644-6653.
[37] Presicce P, Giannelli S, Taddeo A, et al. Human defensins activate monocyte-derived dendritic cells, promote the production of proinflammatory cytokines, and up-regulate the surface expression of CD-91[J]. J Leukoc Biol, 2009, 86(4): 941-948.
[38] Territo MC, Ganz T, Selsted ME, et al. Monocyte-chemotactic activity of defensins from human neutrophils[J]. J Clin Invest, 1989, 84(6): 2017-2020.
[39] Yoshioka M, Fukuishi N, Kubo Y, et al. Human cathelicidin CAP18/LL-37 changes mast cell function toward innate immunity[J]. Biol Pharm Bull, 2008, 31(2): 212-216.
[40] Guryanova SV, Ovchinnikova TV. Immunomodulatory and allergenic properties of antimicrobial peptides[J]. Int J Mol Sci, 2022, 23(5): 2499.
[41] Nagaoka I, Tamura H, Hirata M. An antimicrobial cathelicidin peptide, human CAP18/LL-37, suppresses neutrophil apoptosis via the activation of formyl-peptide receptor-like 1 and P2X7[J]. J Immunol, 2006, 176(5): 3044-3052.
[42] Boniotto M, Jordan WJ, Eskdale J, et al. Human β- defensin 2 induces a vigorous cytokine response in peripheral blood mononuclear cells[J]. Antimicrob Agents Chemother, 2006, 50(4): 1433-1441.
[43] Tewary P, de la Rosa G, Sharma N, et al. β-Defensin 2 and 3 promote the uptake of self or CpG DNA, enhance IFN-α production by human plasmacytoid dendritic cells, and promote inflammation[J]. J Immunol, 2013, 191(2): 865-874.
[44] Bowdish DME, Davidson DJ, Hancock REW. Immunomodulatory properties of defensins and cathelicidins[M]//Current topics in microbiology and immunology. Berlin: Springer, 2006: 27-66.
[45] Zheng Y, Niyonsaba F, Ushio H, et al. Cathelicidin LL-37 induces the generation of reactive oxygen species and release of human α-defensins from neutrophils[J]. Br J Dermatol, 2007, 157(6): 1124-1131.
[46] Chen XX, Zhou L, Wu D, et al. The effects of titanium surfaces modified with an antimicrobial peptide GL13K by silanization on polarization, anti-inflammatory, and proinflammatory properties of macrophages[J]. Biomed Res Int, 2020: 2327034.
[47] Jia FH, Xu DY, Sun YX, et al. Strontium-calcium doped titanium dioxide nanotubes loaded with GL-13K for promotion of antibacterial activity, anti-inflammation, and vascularized bone regeneration[J]. Ceram Int, 2023, 49(22): 35703-35721.
[48] Grover V, Chopra P, Mehta M, et al. Improvisation and evaluation of laterosporulin coated titanium surfaces for dental applications: an in vitro investigation[J]. Indian J Microbiol, 2021, 61(2): 203-211.
[49] Wisdom C, Chen C, Yuca E, et al. Repeatedly applied peptide film kills bacteria on dental implants[J]. JOM, 2019, 71(4): 1271-1280.
[50] Yazici H, O'Neill MB, Kacar T, et al. Engineered chimeric peptides as antimicrobial surface coating agents toward infection-free implants[J]. ACS Appl Mater Interfaces, 2016, 8(8): 5070-5081.
[51] Geng HJ, Yuan Y, Adayi A, et al. Engineered chimeric peptides with antimicrobial and titanium-binding functions to inhibit biofilm formation on Ti implants[J]. Mater Sci Eng C, 2018, 82: 141-154.
[52] Geng HJ, Sun X, Zhang X, et al. Efficient titanium surface modified using bifunctional chimeric pepti-des to prevent biofilm formation by multiple microorganisms[J]. Colloids Surf B Biointerfaces, 2023, 230: 113534.
[53] Godoy-Gallardo M, Mas-Moruno C, Fernández-Cal-derón MC, et al. Covalent immobilization of hLf1-11 peptide on a titanium surface reduces bacterial adhesion and biofilm formation[J]. Acta Biomater, 2014, 10(8): 3522-3534.
[54] Zhou L, Lai YZ, Huang WX, et al. Biofunctionalization of microgroove titanium surfaces with an antimicrobial peptide to enhance their bactericidal acti-vity and cytocompatibility[J]. Colloids Surf B Biointerfaces, 2015, 128: 552-560.
[55] Xu L, Huang ZW. Effect of surface nanomodification of titanium implants on adhesion, bone bon-ding, and bacterial aggregation of gingival epithelial cells and fibroblasts[J]. Sci Adv Mater, 2023, 15(8): 1072-1081.
[56] Mutreja I, Lan CX, Li QS, et al. Chemoselective coatings of GL13K antimicrobial peptides for dental implants[J]. Pharmaceutics, 2023, 15(10): 2418.
[57] Li T, Wang N, Chen S, et al. Antibacterial activity and cytocompatibility of an implant coating consis-ting of TiO2 nanotubes combined with a GL13K antimicrobial peptide[J]. Int J Nanomedicine, 2017, 12: 2995-3007.
[58] Yazici H, Habib G, Boone K, et al. Self-assembling antimicrobial peptides on nanotubular titanium surfaces coated with calcium phosphate for local therapy[J]. Mater Sci Eng C Mater Biol Appl, 2019, 94: 333-343.
[59] Zhou L, Xing YF, Ou YJ, et al. Prolonged release of an antimicrobial peptide GL13K-loaded thermosensitive hydrogel on a titanium surface improves its antibacterial and anti-inflammatory properties[J]. RSC Adv, 2023, 13(33): 23308-23319.
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