Int J Stomatol

Int J Stomatol ›› 2024, Vol. 51 ›› Issue (5): 596-607.doi: 10.7518/gjkq.2024054

• Original Articles • Previous Articles     Next Articles

Antibacterial property of berberine-loaded coaxial electrospun membranes against periodontal pathogens and biofilms

Rui Zhang1(),Ting Hao1,Lü Wen1,Shuangshuang Ren2,Yu Liu3,Wenlei Wu3,Weibin Sun1()   

  1. 1.Dept. of Periodontics, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Research Institute of Stomatology, Nanjing University, Nanjing 210008, China
    2.Dept. of Cariology and Endodontics, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Research Institute of Stomatology, Nanjing University, Nanjing 210008, China
    3.Dept. of Senior Specialist, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Research Institute of Stomatology, Nanjing University, Nanjing 210008, China
  • Received:2024-01-25 Revised:2024-06-13 Online:2024-09-01 Published:2024-09-14
  • Contact: Weibin Sun E-mail:zzzrrr2008@163.com;wbsun@nju.edu.cn
  • Supported by:
    National Natural Science Foundation of China(51972167)

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Abstract:

Objective　This study aimed to prepare berberine-loaded coaxial electrospun membranes, evaluate their antibacterial effects against periodontal pathogens, and explore their inhibitory effect on biofilm formation in vitro. Me-thods　Coaxial polycaprolactone/gelatin electrospun membranes loaded with berberine (drug loading capacity of 0.25%, 0.5% and 1.0%, respectively) were fabrica-ted and characterized. The bacteriostatic zone test was used to evaluate the antibacterial effect against Porphyromonas gingivalis (ATCC 33277) and Fusobacterium nucleatum (ATCC 25586). Crystal violet staining, phenol-sulfuric acid method, colony counting method, and live/dead cell staining were used to comprehensively evaluate the inhibitory effect of drug-loaded electrospun membranes on biofilm formation. The cell counting kit-8 and confocal microscopy me-thods was used to evaluate the biocompatibility of electrospun membranes. Results　The berberine-loaded electrospun membranes had stable physicochemical properties, and they could release berberine in vitro. The membranes (drug loa-ding capacity of 1.0%) exhibited efficient antibacterial activity against Porphyromonas gingivalis and Fusobacterium nucleatum, and inhibited the formation of biofilms in a concentration-dependent manner. The berberine-loaded membranes had good biocompatibility in vitro. Conclusion　The berberine-loaded coaxial electrospun membranes were biocompatible, and they had an antibacterial potential against periodontal pathogens and biofilm formation in vitro. Therefore, it was promising to use berberine-loaded membranes as adjunctive therapy or to prevent postoperative infection.

Key words: berberine, coaxial electrospinning, Porphyromonas gingivalis, biofilm

CLC Number: 

  • R781.4

TrendMD: 

Fig 1

Characterization of the PCL/Gel and PCL/Gel-BBR-H fibrous membranes"

Fig 2

Release profile of berberine from drug-loaded membranes"

Fig 3

Antibacterial property of drug-loaded membranes in vitro"

Fig 4

Biofilm biomass of P. gingivalis and F. nucleatum biofilm under coculture with drug-loaded membranes"

Fig 5

EPS production of P. gingivalis and F. nucleatum biofilm under coculture with drug-loaded membranes"

Fig 6

Bacteria concentration and count of colony formation unit of P. gingivalis and F. nucleatum biofilm under coculture with drug-loaded membranes"

Fig 7

Live/dead staining of P. gingivalis and F. nucleatum biofilm under coculture with drug-loaded membranes"

Fig 8

Surface morphology of P. gingivalis and F. nucleatum biofilm under coculture with drug-loaded membranes SEM"

Fig 9

Relative cell activity under coculture with drug-loaded membranes"

Fig 10

Cell adhesion morphology on surface of the drug-loaded membranes confocal microscope × 10 000"

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