Int J Stomatol

Int J Stomatol ›› 2021, Vol. 48 ›› Issue (3): 305-311.doi: 10.7518/gjkq.2021050

• Original Articles • Previous Articles     Next Articles

Effect of nicotine on the growth of oral bacteria in single or mixed species

Li Shijia(),Chen Qiuyu,Zou Jing,Huang Ruijie()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-09-04 Revised:2021-01-12 Online:2021-05-01 Published:2021-05-14
  • Contact: Ruijie Huang E-mail:2210647879@qq.com;ruijmhuang@gmail.com
  • Supported by:
    Research Initiation Fund for Young Teachers of Sichuan University(2016SCU11042);Undergraduate Innovation and Entrepreneurship Training Program of Sichuan University(C2019107003)

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Abstract:

Objective This work aims to investigate the effect of nicotine on the growth of oral bacteria in single or mixed species. Methods Ten oral bacterial species, Streptococcus mutans, Streptococcus gordonii, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguinis, Staphylococcus aureus, Enterococcus faecalis, Lactobacillus casei, and Staphylococcus epidermidis, were grown in single or mixed species in the presence of different concentrations of nicotine. The number of bacteria in planktonic state and biofilm state was calculated and used to evaluate the regulation of nicotine on bacterial growth. Results In the most cases of the single-species model, nicotine promotes bacterial growth. However, in the mixed-species model, the nicotine and the presence of other bacteria regulate bacterial growth. The growth of cariogenic bacteria, such as Streptococcus mutans, is significantly more active than that of other bacteria. Conclusion Nicotine increases the caries risk of smokers by shifting the oral flora in favor of caries.

Key words: nicotine, oral microorganism, planktonic bacteria, biofilm bacteria, microbial ecology

CLC Number: 

  • R780.2

TrendMD: 

Tab 1

Specific primers for ten bacteria species"

细菌 序列(5’—3’) 模板链
变异链球菌 正向引物 AGTCGTGTTGGTTCAACGGA
反向引物 TAAACCGGGAGCTTGATCGG
格氏链球菌 正向引物 GCCTTAATAGCACCGCCACT
反向引物 CCATCTCTGTTGTTAGGGCGT
缓症链球菌 正向引物 CATCTCACGGGTTGAAGCCT
反向引物 CCTCGCAGACTAAATTCGCC
口腔链球菌 正向引物 GGCCGTGAGAATGTGATTGC
反向引物 TGTTACAGCCTGACCACCAC
唾液链球菌 正向引物 CTGCTCTTGTGACAGCCCAT
反向引物 ACGGGAAGCTGATCTTTCGTA
血链球菌 正向引物 TCAGCAAATCCCCCAGGTTC
反向引物 AACGGAGTGTCAGCGAAGTT
金黄色葡萄球菌 正向引物 TCAGATGAGCAAGCTTCACCAA
反向引物 TGGCTGTACTGCTGCTATACG
粪肠球菌 正向引物 CGCGAACATTTGATGTGGCT
反向引物 GTTGATCCGTCCGCTTGGTA
干酪乳杆菌 正向引物 AAGAAAGGCTCACTGGTCGG
反向引物 TTTTGGCCCGGATTCGATGA
表皮葡萄球菌 正向引物 CATATGGACCTGCACCCCAA
反向引用 GCAACTGCTCAACCGAGAAC

Fig 1

Growth of 10 species of bacteria after individual planktonic culture for 24 h"

Fig 2

Growth of 10 species of bacteria after mixed planktonic culture for 24 h"

Fig 3

Proportion of 10 species of bacteria after mixed planktonic culture for 24 h with different concentrations of nicotine"

Fig 4

Biofilm formation of 10 species of bacteria after individual culture for 24 h"

Fig 5

Biofilm formation of 10 species of bacteria after mixed culture for 24 h"

Fig 6

Proportion of biofilm of 10 species of bacteria after mixed culture for 24 h with different concentrations of nicotine"

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