Inter J Stomatol ›› 2017, Vol. 44 ›› Issue (4): 421-425.doi: 10.7518/gjkq.2017.04.010

• Original Articles • Previous Articles     Next Articles

Effect of nicotine and mecamylamine on growth of periodontal pathogens

Liu Shiyu, Tian Mi, Shi Liran, Pan Weilin, Wang Yiyao, Li Mingyun   

  1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2016-07-22 Revised:2017-04-13 Online:2017-07-01 Published:2017-07-01
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(81400501), the Special Funds of State Key Laboratory of Oral Diseases(SKLOD201525) and Sichuan University Students’ Innovative Training Program(20140298).

Abstract: Objective This work aims to study the effects of nicotine and mecamylamine on periodontal pathogen growth and pathogen-formed biofilms. Methods Doubling dilution was used to determine the minimum inhibition concentration(MIC) and minimum bactericidal concentration(MBC) of nicotine on Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans. The influences of 1/16 MIC, 1/8 MIC, 1/4 MIC, 1/2 MIC, and MIC of nicotine on the biofilms formed by P. gingivalis and A. actinomycetemcomitans were studied by light absorbance determination on a microplate reader. Results The MIC of nicotine against planktonic P. gingivalis was 8 mg·mL-1, which was similar to that of A. actinomycetemcomitans. When the nicotine concentration increased by a degree, the absorbance of the two bacterial biofilms increased. However, the absorbance decreased at further increase in nicotine concentration. The absorbance decreased to a minimum at a nicotine concentration of 8 mg·mL-1, which was also the minimum biofilm inhibition concentration. The group with mecamylamine(nicotine-receptor antagonist) showed higher absorbance than the control group. Conclusion Minimal doses of nicotine can promote the biofilm formation of P. gingivalis and A. actinomycetemcomitans, and continued increase in nicotine concentration inhibits biofilm formation. In the presence of mecamylamine, nicotine increases the biofilms formed by the P. gingivalis and A. actinomycetemcomitans. This effect is attributed to the decrease in nicotine concentration caused by mecamylamine.

Key words: nicotine, mecamylamine, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, biofilm

CLC Number: 

  • R780.2

[1] Petersen PE, Bourgeois D, Ogawa H, et al. The glo-bal burden of oral diseases and risks to oral health[J]. Bull World Health Organ, 2005, 83(9):661-669.
[2] Mascarenhas P, Gapski R, Al-Shammari K, et al. Clinical response of azithromycin as an adjunct to non-surgical periodontal therapy in smokers[J]. J Periodontol, 2005, 76(3):426-436.
[3] 刘颍凤, 王小竞, 吴礼安. 大鼠尼古丁实验性牙周炎动物模型的建立[J]. 牙体牙髓牙周病学杂志, 2008, 18(3):148-152.
Liu YF, Wang XJ, Wu LA. Establishment of nicotine experimental periodontitis model in rats[J]. Chin J Conserv Dent, 2008, 18(3):148-152.
[4] Bouclin R, Landry RG, Noreau G. The effects of smoking on periodontal structures: a literature re-view[J]. J Can Dent Assoc, 1997, 63(5):360-363.
[5] Li M, Huang R, Zhou X, et al. Effect of nicotine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis [J]. FEMS Microbiol Lett, 2014, 350(2):125-132.
[6] Teughels W, Van Eldere J, Van Steenberghe D, et al. Influence of nicotine and cotinine on epithelial colo-nization by periodontopathogens[J]. J Periodontol, 2005, 76(8):1315-1322.
[7] An N, Andrukhov O, Tang Y, et al. Effect of nicotine and Porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro [J]. PLoS One, 2014, 9(5):e96942.
[8] Cogo K, De Andrade A, Labate CA, et al. Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine[J]. J Periodontal Res, 2012, 47 (6):766-775.
[9] 江海. 烟碱对M胆碱能受体功能的调节及分子机理[J]. 生理科学进展, 1994, 25(3):240-242.
Jiang H. Regulation and molecular mechanism of nicotine on the function of M cholinergic receptor[J]. Advan Physiol Sci, 1994, 25(3):240-242.
[10] Rose JE, Behm FM, Westman EC, et al. Mecamy-lamine combined with nicotine skin patch facilitates smoking cessation beyond nicotine patch treatment alone[J]. Clin Pharmacol Ther, 1994, 56(1):86-99.
[11] Li C, Hu B, Bian J, et al. Abrogation of neuramini-dase reduces biofilm formation, capsule biosynthesis, and virulence of Porphyromonas gingivalis [J]. Infect Immun, 2012, 80(1):3-13.
[12] Haase EM, Bonstein T, Palmer RJ, et al. Environ-mental influences on Actinobacillus actinomycetem comitans biofilm formation[J]. Arch Oral Biol, 2006, 51(4):299-314.
[13] Kenney EB, Saxe SR, Bowles RD. The effect of cigarette smoking on anaerobiosis in the oral cavity [J]. J Periodontol, 1975, 46(2):82-85.
[14] 潘亚萍, 刘静波. 牙龈卟啉单胞菌研究进展[J]. 国际口腔医学杂志, 2011, 38(2):125-127.
Pan YP, Liu JB. Research progress on Porphyro-monas gingivalis [J]. Int J Stomatol, 2011, 38(2): 125-127.
[15] 黄定明, 吴亚菲, 周学东. 牙龈卟啉单胞菌的分型及其致病作用[J]. 国外医学: 口腔医学分册, 2002, 29(4):213-215.
Huang DM, Wu YF, Zhou XD. Classification and pathogenicity of Porphyromonas gingivalis [J]. Foreign Med Sci: Stomatol, 2002, 29(4):213-215.
[16] 张迪亚, 李盛来. 伴放线放线杆菌与牙周炎相关细胞凋亡关系的研究[J]. 国际口腔医学杂志, 2007, 34(2):94-97.
Zhang DY, Li SL. Research development of the relationship between Actinobacillus actinomyce - temcomitans and apoptosis in periodontitis[J]. Int J Stomatol, 2007, 34(2):94-97.
[17] 孟姝, 吴亚菲, 杨禾. 伴放线放线杆菌产生的细胞致死膨胀毒素及其与牙周病的关系[J]. 国外医学:口腔医学分册, 2005, 32(6):458-460.
Meng S, Wu YF, Yang H. The relationship between cell death toxin(CDT) produced by Actinobacillus actinomycetemcomitans and periodontal diseases[J]. Foreign Med Sci: Stomatol, 2005, 32(6):458-460.
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