国际口腔医学杂志

国际口腔医学杂志 ›› 2025, Vol. 52 ›› Issue (5): 594-605.doi: 10.7518/gjkq.2025061

• 牙体牙髓病学专栏 • 上一篇    下一篇

基于支架的牙髓组织预血管化技术的研究进展

李甜1,2(),李丽洁1,2()   

  1. 1.内蒙古医科大学附属医院口腔科 呼和浩特 010050
    2.内蒙古医科大学口腔医学院 呼和浩特 010059
  • 收稿日期:2024-08-06 修回日期:2024-10-07 出版日期:2025-09-01 发布日期:2025-08-27
  • 通讯作者: 李丽洁
  • 作者简介:李甜，医师，硕士，Email：1785708725@qq.com

Research progress on scaffold-based prevascularization technique for dental pulp tissue

Tian Li1,2(),Lijie Li1,2()   

  1. 1.Dept. of Stomatology, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China
    2.School of Stomatology, Inner Mongolia Medical University, Hohhot 010059, China
  • Received:2024-08-06 Revised:2024-10-07 Online:2025-09-01 Published:2025-08-27
  • Contact: Lijie Li

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摘要：

对于牙髓坏死的年轻恒牙，目前临床上多采用牙髓血运重建术进行治疗。因该方法无法重建功能性牙髓样组织，并可能导致根管钙化，因此再生牙髓治疗引起了广泛关注。牙髓组织再生的目的是恢复自体牙髓组织的活性与功能，其成功的关键因素是快速形成功能性血管网。根管和髓腔作为一种相对狭窄、密闭、缺血缺氧的环境，功能性血管网从根管长到髓腔的过程非常缓慢，因此预血管化技术应运而生。该技术是在牙髓的工程化组织构建中预先形成功能性血管网络，确保为构建体提供足够的血液供应；然后将血管网络与宿主脉管系统吻合，最终诱导血管化牙髓组织再生。本文综述了基于支架的预血管化技术在细胞、生长因子和支架方面的研究进展，同时也总结了与预血管化相关的三维生物打印技术及其应用，以期为再生医学发展提供理论依据。

关键词: 牙髓组织工程, 牙髓组织再生, 预血管化技术, 支架, 三维生物打印

Abstract:

In young permanent teeth with pulp necrosis, the current clinical approach for pulp revascularization does not effectively reconstruct functional pulp-like tissue and may lead to root canal calcification. Consequently, regenerative endodontic treatment has garnered widespread attention from scholars. The primary aim of dental pulp tissue regeneration is to restore the activity and functionality of autologous dental pulp tissue, with the rapid formation of a functional vascular network taken as a critical success factor. However, the root canal and medullary cavity present relatively narrow, airtight, ischemic, and hypoxic environments, which impedes the growth of the functional vascular network from the root canal to the medullary cavity. This challenge has been addressed by researchers who proposed prevascularization technique, which involves the formation of a functional vascular network within engineered tissue to ensure an adequate blood supply to the construct. This vascular network can anastomose with the host vasculature, ultimately facilitating the regeneration of vascularized dental pulp tissue. This article reviews potential cell types, growth factors, and scaffold sources pertinent to scaffold-based prevascularization technology. Three dimension (3D) bioprinting technology related to prevascula-rization is discussed, and a theoretical foundation for advancements in regenerative medicine is provided.

Key words: pulp tissue engineering, pulp tissue regeneration, prevascularization technique, scaffold, three dimension bioprinting

中图分类号: 

  • R781
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