国际口腔医学杂志

国际口腔医学杂志 ›› 2023, Vol. 50 ›› Issue (3): 321-328.doi: 10.7518/gjkq.2023044

• 综述 • 上一篇    下一篇

基于唾液代谢组学的口腔疾病研究新进展

杨倩娟1(),宋致馨1,方世殊2,顾泽旭1,金作林1,刘倩1()   

  1. 1.空军军医大学第三附属医院口腔正畸科;军事口腔医学国家重点实验室;国家口腔疾病临床医学研究中心 陕西省口腔疾病临床医学研究中心 西安 710032
    2.中国人民解放军南部战区总医院口腔科 广州 510010
  • 收稿日期:2022-11-05 修回日期:2023-03-10 出版日期:2023-05-01 发布日期:2023-05-16
  • 通讯作者: 刘倩
  • 作者简介:杨倩娟，住院医师，硕士，Email：kq1966@126.com
  • 基金资助:
    陕西省重点研发计划项目(2021SF-048);国家口腔疾病临床医学研究中心2020年度专项课题A类项目(LCA202009)

New advances in oral diseases based on salivary metabolomics

Yang Qianjuan1(),Song Zhixin1,Fang Shishu2,Gu Zexu1,Jin Zuolin1,Liu Qian1()   

  1. 1.Dept. of Orthodontics, School of Stomatology, The Air Force Medical University, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi’an 710032, China
    2.Dept. of Stomatology, General Hospital of Southern Theater Command of the Chinese People’s Liberation Army, Guangzhou 510010, China
  • Received:2022-11-05 Revised:2023-03-10 Online:2023-05-01 Published:2023-05-16
  • Contact: Qian Liu
  • Supported by:
    Key Research and Development Program of Shaanxi Province(2021SF-048);Class A Project of 2020 Special Project of the National Clinical Research Center for Oral Diseases(LCA202009)

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摘要：

唾液代谢组学是以唾液为研究对象，定性定量分析生物体内代谢物的变化以研究生物体疾病与健康的新兴学科。在口腔医学领域，采用唾液代谢组学研究发现，唾液代谢物与多种口腔疾病之间存在着联系。本文对近年来以唾液代谢组学为研究手段进行口腔疾病相关研究的进展进行综述，包括在口腔鳞状细胞癌、口腔黏膜癌前病变（口腔扁平苔藓和口腔黏膜白斑）、复发性阿弗他溃疡、原发性舍格伦综合征、早期儿童龋病、牙周炎、慢性根尖周炎、正畸后牙根吸收等方面的研究，对通过唾液代谢组学筛选出的相关差异代谢物和信号通路进行总结，通过这些差异代谢物，可以构建口腔疾病预测模型，对口腔疾病进行早期筛查、预后判断以及发病机制的研究。唾液代谢组学有望为口腔疾病的诊断提供一种无创性的方法，具有重要的临床意义。

关键词: 唾液代谢组学, 口腔疾病, 生物标记物

Abstract:

Salivary metabolomics is a newly developing subject that investigates the changes of metabolites in orga-nisms qualitatively and quantitatively in order to study the disease and health of organisms with saliva as the research object. Studies in the field of stomatology found some connection issues between saliva metabolites and diverse oral disea-ses. This article reviews the recent progress in oral-disease-related research that uses salivary metabolomics as a research method, including oral squamous cell carcinoma, oral mucosa precancerous lesion (oral lichen planus and oral leukoplakia), recurrent Aphthous ulcer, primary Sjogren syndrome, early childhood caries, periodontitis, chronic periapical inflammation, and root resorption after orthodontics. By summarizing the relevant differential metabolites and signaling pathways selected by saliva metabolomics, oral disease prediction models can be built through these differential metabolites to carry out early screening, prognosis judgment, and the study of the pathogenesis of oral diseases. Saliva metabolomics is expected to provide a non-invasive method for the diagnosis of oral diseases, which has important clinical significance.

Key words: salivary metabolomics, oral disease, biomarkers

中图分类号: 

  • R 78

表 1

基于唾液代谢组学的口腔疾病诊断的研究方法、差异代谢物和代谢途径"

研究

疾病

作者研究对象研究方法差异代谢物相关代谢途径
表达上调表达下调
OSCCWang等[11]OSCC组及健康对照组各30例UPLC-MS乳酸、羟基苯乳酸、N-壬酰基甘氨酸、5-羟甲基尿嘧啶、丁二酸、鸟氨酸、己酰肉碱、丙酰胆碱、肉碱4-羟基-L-谷氨酸、N-乙酰-L-苯丙氨酸、鞘氨醇、植物鞘氨醇、S-羧甲基-L-半胱氨酸糖酵解、酪氨酸代谢、脂肪酸氧化、三羧酸循环、尿素循环、β长链脂肪酸的氧化、精氨酸和脯氨酸代谢、苯丙胺酸代谢、鞘脂代谢等
薛佳艺等[14]OSCC组25例，健康对照组20例GC-TOF-MS酪氨酸、乳酸尿酸、核糖、2-羟基丁二酸三羧酸循环等
Song等[10]OSCC组125例，健康对照组124例CPSI-MS乙酰腐胺、 乙酰胺、哌啶、尸胺、腐胺、N1-乙酰精胺、亚精胺、瓜氨酸、5-氨基戊酸、尿刊酸氧化精氨酸、脯氨酸、N-乙酰精胺、二乙酰亚精胺、2-哌啶酸、赖氨酸、精胺、精氨酸、鸟氨酸、组氨酸氨酰基-tRNA的生物合成、精氨酸/脯氨酸代谢、精氨酸生物合成、赖氨酸降解、组氨酸代谢
OSCC和OLPIshikawa等[16]OSCC组34例，OLP组26例CE-MSN-氧化三甲胺、丁二胺、肌酐、5‐氨基戊酸酯、甲基哌啶、N-乙酰基腐胺、γ-丁酰甜菜碱、吲哚-3-乙酸、N1‐乙酰精胺、2’‐脱氧肌苷、磷酸乙醇胺、N-乙酰基葡糖胺N-乙酰-L-组氨酸、乙酰左旋肉碱鞘脂代谢、精氨酸/脯氨酸代谢、谷胱甘肽代谢、赖氨酸分解、甘油磷脂代谢、嘌呤代谢
OLP王晓霜[18]OLP 120例，健康受试者125例UHPLC-Q-Orbitrap HRMS谷氨酸、焦谷氨酸、柠檬酸、牛磺酸、鸟氨酸、尿酸、乙酰左旋肉碱、次黄嘌呤、磷酸、肌酸、磷酰乙醇胺等植物鞘氨醇d-谷氨酰胺代谢，d-谷氨酸代谢，牛磺酸和次牛磺酸代谢，精氨酸和脯氨酸代谢，丙氨酸、天门冬氨酸和谷氨酸代谢，精氨酸生物合成，甘油磷脂代谢
OLKWei等[19]OLK 32例，健康受试者34例Q/TOF-MS异亮氨酸、N-四癸酸、苯氨基丙酸、苏氨酸、同型半胱氨酸、4-甲氧基苯乙酸酸
Sridharan等[20]OLK 21例，健康受试者18例Q/TOF—LC/MS甲基组氨酸、三磷酸肌醇、甘油酸二磷酸、氧化4-硝基喹啉-1-氧化物、氧化精氨酸、去甲可卡因硝基氧、1-磷酸鞘氨醇、假尿嘧啶L-同型半胱氨酸、泛醌、神经氨酸、戊酸雌二醇碳水化合物代谢、核苷酸代谢、神经鞘脂代谢、雌激素代谢和蛋白质信号代谢
RAULi等[21]RAU 45例，健康受试者49例LC-MS/MS色胺、5-甲氧基色胺硫酸雌酮、硫酸脱氢表雄酮色氨酸代谢、类固醇激素生物合成和其他代谢途径
舍格伦综合征Herrala等[24]舍格伦综合征14例，健康受试者15例NMRS胆碱、牛磺酸、甘氨酸、丙氨酸
早期儿童龋病Schulz等[31]龋病儿童21例，龋病已充填儿童18例，健康儿童18例UPLC-MS/MS焦糖、半乳糖、葡萄糖、甘露糖、棕榈酸、亚油酸、油酸、硬脂酸盐
慢性根尖周炎Montis等[35]慢性根尖周炎并发窦道者11例，健康对照者8例GC/MC4-羟基肉桂酸、N-乙酰神经氨酸、类肌醇、鸟氨酸、腐胺、次黄嘌呤、5-氨基戊酸、脯氨酸、尿嘧啶、赖氨酸、硬脂酸、苏氨酸、尿酸、甘氨酸和磷酸乙醇胺山梨醇、麦芽糖、葡萄糖、木糖醇、琥珀酸、乙醇胺、乳酸、棕榈酸、柠檬酸、尿素、丙二醇和丁二醇嘌呤核苷酸循环、氨基糖和核苷酸代谢、戊糖磷酸途径、糖酵解和糖异生途径
OIEARRZhou等[36]OIEARR患者8例，健康对照者11例NMRS丁酸、丙烷-1,2-二醇、α-亚麻酸、α-葡萄糖、尿酸、富马酸、甲酸盐、鸟苷、嘌呤等

《中国口腔数字化——从临床技术到病例精选》出版发行"

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