Int J Stomatol

Int J Stomatol ›› 2021, Vol. 48 ›› Issue (5): 520-527.doi: 10.7518/gjkq.2021075

• Original Articles • Previous Articles     Next Articles

Effects of platelet-rich plasma and concentrated growth factor on the proliferation and osteogenic differentiation of human periodontal cells

Liu Juan(),Chen Bin,Yan Fuhua()   

  1. Dept. of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
  • Received:2021-02-03 Revised:2021-06-02 Online:2021-09-01 Published:2021-09-10
  • Contact: Fuhua Yan E-mail:1044042989@qq.com;yanfh@nju.edu.cn
  • Supported by:
    National Natural Science Foundation of China(81771078);Project of Nanjing Clinical Research Center for Oral Diseases(2019060009);Key Project of Science and Technology Bureau of Jiangsu Province(BL2013002)

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Abstract:

Objective The study aimed to analyze the effects of platelet-rich plasma (PRP) and concentrated growth factor (CGF) on the proliferation and osteogenic differentiation of human periodontal membrane cells (hPDLCs). Metho-ds The hPDLCs were cultured and isolated from periodontal ligament tissue. PRP and CGF were prepared in accordance with the standard protocols, and platelet count was performed separately. The hPDLCs were cultured to three groups: whole blood (control group), PRP (PRP group), and CGF (CGF group) at various time points. Cell proliferation was detected by the CCK-8 method, and cell migration was detected by the cell scratch assay. The hPDLCs were treated with 1%, 5%, and 10% of PRP and CGF for 24, 48, and 72 h, respectively, for osteogenic induction. Expression levels of osteogenic transcription factors Runx2, Osx, Dlx5, and Msx2 were detected by Western blot. Results Both PRP and CGF could promote the proliferation and migration of the hPDLCs (P<0.05). The expression levels of osteogenic factors Runx2, Osx, and Dlx5 increased significantly; the expression of Msx2, which inhibited osteogenesis, decreased significantly (P<0.05). Conclusion PRP and CGF could promote the proliferation and migration of hPDLCs and induce the expression of osteogenic differentiation-related transcription factors.

Key words: platelet-rich plasma, concentrated growth factor, human periodontal ligament cells, cell proliferation, osteogenic differentiation

CLC Number: 

  • Q25

TrendMD: 

Fig 1

The preparation of CGF from the whole blood"

Fig 2

The isolation culture and identification of hPDLCs"

Tab 1

The platelet count of whole blood, PRP and CGF"

样本编号 志愿者基本资料 血小板计数/（109·L-1）
性别 年龄/岁 全血 PRP CGF
1 男 22 278 2 459 –
2 女 22 190 1 305 –
3 女 23 193 2 558 –
4 男 26 263 2 553 –
5 女 25 210 3 127 –
6 男 24 269 1 953 –
7 女 23 237 2 044 –

Fig 3

The effect of PRP and CGF on the proliferation ability of hPDLCs"

Fig 4

The effect of PRP and CGF on the migration ability of hPDLCs crystal violet staining"

Fig 5

The effect of PRP and CGF on the expression of Msx2, Osx, Dlx5 and Runx2 detected with Western blot"

Fig 6

The effect of PRP and CGF on the expression levels of Runx2, Dlx5, Osx and Msx2"

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