Int J Stomatol

Int J Stomatol ›› 2024, Vol. 51 ›› Issue (1): 45-51.doi: 10.7518/gjkq.2024016

• Original Articles • Previous Articles     Next Articles

The correlation study between single nucleotide polymorphisms of Toll-like receptor and interleukin-17 and oral lichen planus

Li Ran1,2(),Hu Yue3,Zhang Ning4,Gao Ruifang1,2,Wang Xiangyu1,2,Ge Xuejun2()   

  1. 1.Dept. of Pediatric and Preventive Dentistry, Shanxi Medical University School & Hospital of Stomatology, Taiyuan 030001, China
    2.Shanxi Medical University School & Hospital of Stomatology, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, China
    3.Dept. of Pediatric Dentistry, Homony Long Stomatological Hospital, Taiyuan 030006, China
    4.Dept. of Stomatology, Emergency General Hospital, Beijing 100028, China
  • Received:2023-04-07 Revised:2023-10-09 Online:2024-01-01 Published:2024-01-10
  • Contact: Xuejun Ge E-mail:lraner@163.com;gxj19722003@163.com
  • Supported by:
    Shanxi Province Basic Research Program(202103021223235)

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Abstract:

Objective This study aimed to explore the genetic polymorphisms of the Toll-like receptor (TLR) and interleukin (IL)-17 genes and their correlation with oral lichen planus (OLP). Methods DNA extracted from the peripheral blood of patients in the case (OLP group) and control (non-OLP population) groups were selected as the research subjects. Seven single nucleotide polymorphisms were attempted in our study, namely, rs5743312 in TLR3, rs121917864 in TLR2, and five others (rs4711998, rs3819024, rs8193036, rs3748067, and rs17878530) in IL-17A. High resolution melting curve analysis was used to detect the gene polymorphism of PCR products in peripheral blood cells and compared with DNA sequencing. The correlation between polymorphic sites and the occurrence of OLP was statistically analyzed. Results We found that rs5743312 (TLR3) and rs3819024 (IL-17A) had a statistically significant association with OLP (P<0.05). The remaining five SNPs cannot be tested for association with OLP. Conclusion rs5743312 (TLR3) and rs-3819024 (IL-17A) may contribute to the initiation of OLP.

Key words: single nucleotide polymorphism, high resolution curve melting analysis, oral lichen planus, susceptibility

CLC Number: 

  • R781.5

TrendMD: 

Tab 1

Baseline characteristics of patients and controls"

纳入临床指标病例组（n=83）对照组（n=87）P值
男/女19/6422/650.715 0
平均年龄/岁47.8846.910.630 7
年龄范围/岁17~7821~60
病程/月1.0~36.0
病程均数/月11.3
糜烂型/n55
非糜烂型/n28

Tab 2

HWE test of rs5743312, rs4711998 and rs3819024"

检验参数rs5743312rs4711998rs3819024
基因型CT/TTAG/GGAG/GG
n27/1334/1643/5
χ2值5.261.743.05
P值0.072 20.418 70.217 1

Fig 1

Curves of high resolution melting analysis"

Fig 2

Curves of different SNPs"

Fig 3

Chromatogram showing sequencing of SNPs"

Tab 3

Allele frequency distribution and gene polymorphism analysis of OLP with rs5743312, rs4711998 and rs381902"

SNP组别例数/n等位基因型（n/%）χ2值P值OR95% CI
C/GT/A
rs5743312病例组83113/68.053/32.06.8460.0091.9311.175, 3.174
对照组87140/80.034/20.0
rs4711998病例组8366/39.8100/60.20.8020.3701.2230.787, 1.094
对照组8761/35.1113/64.9
rs3819024病例组8353/31.9113/68.112.91<0.0010.4480.288, 0.697
对照组8789/51.185/48.9

Tab 4

Gene polymorphism analysis of OLP with rs5743312, rs4711998 and rs3819024"

SNP组别例数/n基因型（n/%）χ2值P值OR95% CI
次要等位纯合型至少含1个主要等位基因性型
rs5743312病例组8313/15.770/84.34.4110.0363.0461.035, 8.965
对照组875/10.682/89.4
rs4711998病例组8316/19.367/80.81.3990.2371.650.716, 3.803
对照组8711/12.676/87.4
rs3819024病例组835/6.078/94.015.076<0.0016.290.058, 0.439
对照组8725/28.762/71.3
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