Int J Stomatol

Inter J Stomatol ›› 2017, Vol. 44 ›› Issue (6): 654-659.doi: 10.7518/gjkq.2017.06.006

• Microbiology • Previous Articles     Next Articles

The influence of exogenous dextranase and sodium fluoride on biofilm of Actinomyces viscosus under different sucrose concentration conditions

Fang Hongzhi1, Tian Yuanyuan1,2, Yu Xuan2,3, Yang Yingming4, Yang Hui5, Hu Tao4   

  1. 1. Dept. of Stomatology, The Third People’s Hospital of Chengdu, Chengdu 610031, China;
    2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;
    3. Dept. of Stomatology, Ningbo Yinzhou People’s Hospital, Ningbo 315040, China;
    4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;
    5. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of General Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2016-12-29 Revised:2017-08-21 Online:2017-11-01 Published:2017-11-01
  • Supported by:
    This study was supported by National Natural Science Foundation of China(81400507) and Applied Basic Re-search Project of Sichuan Provincial Science and Technology Department(2013JY0164).

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Abstract: Objective To explore the impact of exogenous dextranase(Dex) and sodium fluoride(NaF) on the biomass, biofilm vitality and acid production of Actinomyces viscosus in different sucrose concentration substrate. Methods Crystal violet staining was used to quantify the biomass of A. viscosus in each group. 2,3 bis(2 methoxy 4 nitro 5 sulfophenyl) 5 [(phenylamino)carbonyl] 2H tetrazolium hydroxide(XTT) assay was used to detect the vitality of the bacterial biofilms. pH electrode was applied to measure the pH values of the initial(pH1) and the 18 h liquid culture medium(pH2), and then the ΔpH(pH1-pH2) values was calculated. Results Under different sucrose concentration conditions, the bacterial biomass increased with the increase of sucrose concentration, and reached the peak at a sucrose mass fraction of 0.5%, and then decreased with the increase of sucrose concentration. In the 1% sucrose mass fraction groups, the bacterial biomass formed in the Dex+NaF group was significantly less than in the Dex or NaF group. In all groups, the bacterial vitality was decreased along with the increased sucrose concentration. Under the same sucrose concentration, the bacteria vitality of the Dex group was similar to that of the control group(P>0.05), and there was a slight increase in the bacteria vitality in the NaF group compared with the control group(P<0.05). With the increase of sucrose concentration, ΔpH changes were first increased and then decreased. The single or combined use of NaF and Dex could inhibit the acid production of A. viscosus under the sucrose mass fraction ranged from 0.25% to 2%. Conclusion Sucrose concentration was one of the factors that can influence the effect of Dex and NaF, both single and combination, on the bacterial biomass, biofilm vitality and acid production. The inhibitory effect of the combined use of Dex and NaF on the formation of bacterial biofilm showed a synergistic effect at the sucrose mass fraction of 1%. Dex and NaF displayed synergistic effect on acid production at the sucrose mass fraction of 2%.

Key words: dextranase, sodium fluoride, biofilm, sucrose, Actinomyces viscosus

CLC Number: 

  • R37

TrendMD: 
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