Int J Stomatol

Int J Stomatol ›› 2025, Vol. 52 ›› Issue (2): 210-216.doi: 10.7518/gjkq.2025029

• Original Articles • Previous Articles     Next Articles

Mechanism of action of aureobasidin A against Candida albicans based on transcriptomics analysis

Jiaxin Yi1(),Jianchun Liao2,Yi Huang3,Wei Fei4,Jun Guo3()   

  1. 1.Dept. of Stomatology, School of Medicine University of Electronic Science and Technology of China, Chengdu 610072, China
    2.Dept. of Stomatology, Luojiang District People’s Hospital in Deyang, Deyang 618000, China
    3.Dept. of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China
    4.Dept. of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (Wenjiang Campus), Chengdu 611100, China
  • Received:2024-08-30 Revised:2024-12-11 Online:2025-03-01 Published:2025-03-01
  • Contact: Jun Guo E-mail:yijiaxin1999@163.com;guojun-001@qq.com
  • Supported by:
    Sichuan Provincial Cadre Health Care Research Topic(2022-209)

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Abstract:

Objective This study aimed to investigate the antibacterial activity and potential mechanism of aureobasi-dium A (AbA) against Candida albicans (C. albicans), as well as to evaluate its potential as a new antifungal drug in the future. Methods The minimum inhibitory concentration (MIC) of AbA was determined. The effect of MIC on mycelium formation and cell-wall integrity were investigated. Subsequently, transcriptome sequencing was used to investigate the response mechanism of C. albicans to AbA. Results In vitro results showed that the MIC of AbA was 0.062 5 μg/mL, and it significantly inhibited C. albicans morphogenesis and disrupted cell-wall integrity. Transcriptome sequencing identified 700 differentially expressed genes, including 465 upre-gulated and 235 downregulated genes. Gene ontology enrichment analysis revealed that pathways related to the extracellular region, plasma membrane, fungal cell wall, and redox enzyme activity were the most enriched. Enrichment analysis through the kyoto encyclopedia of genes and genomes indicated that the metabolic pathway was the most significantly enriched pathway. Conclusion AbA exhibited strong inhibitory activity against C. albicans. Its potential antimicrobial mechanisms may include inhibiting hyphal formation, disrupting the plasma membrane, reducing immune evasion, and suppressing metabolic activity. Understanding these mechanisms can provide valuable theoretical insights into the development of more effective antifungal-treatment strategies.

Key words: Candida albicans, aureobasidin A, transcriptome sequencing, kyoto encyclopedia of genes and genomes enrichment analysis, gene ontology enrichment analysis

CLC Number: 

  • R780.2

TrendMD: 

Tab 1

Primer information"

引物名称引物序列（5’—3’）
ALS3-FTACGCAACCACTACCATTACC
ALS3-RCACCTGGAGCAGTGATTGTAG
HWP1-FGCTCCTGCCACTGAACCTTCC
HWP1-RTTGGAGAAGAAGAAGCACCTGGAC
EFG1-FCGTTGCTGCTGCTACTACTACTG
EFG1-RTGAGGATACTGCTGTTGGTTGTAAG
EAP1-FGGGATGATTACAAGAAAG
EAP1-RGTGACGGTGATGATAGTG
TUP1-FCAACAGCAACAGCAGCAACAAC
TUP1-RACAACTGACGAGTGGTCTAAGGAG
GSP1-FTGAAGTCCATCCATTAGGAT
GSP1-RATCTCTATGCCAGTTTGGAA

Fig 1

The effect of AbA on the hyphal formation of Candida albicans observed under an inverted microscope"

Fig 2

Effect of AbA on the morphology and hyphal formation of Can-dida albicans SEM ×1 000"

Fig 3

Volcanic map of differential gene distribution of Candida albicans between experimental group and AbA treated group"

Fig 4

GO enrichment analysis of differential gene expression in Candida albicans before and after AbA treatment"

Fig 5

KEGG enrichment analysis of differential gene expression in Candida albicans before and after AbA treatment"

Fig 6

Statistical difference of ALS3, HWP1, EFG1, EAP1, TUP1 gene expression after AbA treatment"

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