Int J Stomatol

Inter J Stomatol ›› 2015, Vol. 42 ›› Issue (4): 391-394.doi: 10.7518/gjkq.2015.04.004

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Lipopolysaccharides upregulated the expression of B-cell lymphoma-2 and its associated X protein in human dental pulp cells

Zhu Yuting, Liu Jiangfeng, Li Xiaoxing, Yang Huixiao, Huang Jiangyong, Yu Miao, Chen Bingxun, Li Yanli.   

  1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China
  • Online:2015-07-01 Published:2015-07-01

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Abstract:

Objective To study the effects of lipopolysaccharides(LPS) on B-cell lymphoma-2(Bcl-2) protein and Bcl-2-associated X protein(Bax) expression of human dental pulp cells. Methods In vitro model of human dental pulp cells apoptosis was established by LPS. The protein expression of Bcl-2 and Bax in human dental pulp cell(HDPC) after induction of LPS with different concentrations at different times was determined by immunocytochemical method. The result was analyzed using Simple PCI version 5.1. Results A small number of Bcl-2 and Bax positive cells were observed in normal dental pulp. By contrast, Bcl-2 and Bax showed enhanced expression in inflammatory dental pulp and some pulp cells in the inflammatory area. Bax was furtherexpressedas the LPS increased, whereas Bcl-2 showeda weakened shift. Conclusion The apoptosis in dental pulp induced by Bcl-2 and Bax may play a role in pulpitis. Endotoxin may change the expression of Bcl-2 and Bax on human dental pulp cell membrane and induces cell apoptosis through Bcl-2 and Baxmediated signal pathway.

Key words: pulpitis, cell apoptosis, lipopolysaccharide, B-cell lymphoma-2 protein


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