Abstract: Objective The aim of this study was to observe the effect of 2-hydroxy-propyl trimethyl ammonium chloride chitosan（HTCC） and basic fibroblast growth factor（bFGF） on production of interleukin（IL）-1β and tumor necrosis factor（TNF）-α in human periodontal ligament fibroblast（hPDLF） stimulated by lipopolysaccharide（LPS）. Methods The levels of IL-1β and TNF-α in hPDLF stimulated by 50 mg·L^－1 LPS was observed and the effects of 1 g·L^－1 HTCC and 100 μg·L^－1 bFGF on the IL-1β and TNF-α were determined by enzyme-linked immunosorbent assay. Results 1 g·L^－1 HTCC can stimulate the level of IL-1β and TNF-α in hPDLF stimulated by LPS and the level of cytokines were highest at 48 h. 100 μg·L－1 bFGF can decrease the IL-1β and TNF-α. The level
of IL-1β and TNF-α in hPDLF stimulated by LPS was statistically decreased by association of HTCC and bFGF
（P≤0.001）. Conclusion HTCC can increase the production of IL-1β and TNF-α in hPDLF stimulated by LPS.
And HTCC associated with bFGF can effectively inhibit the level of IL-1β and TNF-α of hPDLF.

Key words: 2-hydroxy-propyl trimethyl ammonium chloride chitosan, human periodontal ligament fibroblast, lipopolysaccharide, interleukin-1β, tumor necrosis factor-α