Int J Stomatol

Inter J Stomatol ›› 2013, Vol. 40 ›› Issue (5): 568-571.doi: 10.7518/gjkq.2013.05.003

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Expression of T-bet, GATA-3, and Foxp3 in peripheral blood of patients with recurrent aphthous ulcer

Nie Yujie1,2, Cai Yang1, Feng Qiujing3.   

  1. 1. Dept. of Stomatology, Guiyang Medical College, Guiyang 550004, China; 2. Dept. of Stomatology, The Second People’s Hospital of Nanhai District in Foshan, Foshan 528251, China; 3. Dept. of Stomatology, The People’s Hospital of Xingyi, Xingyi 562400, China
  • Received:2012-03-11 Revised:2013-04-12 Online:2013-09-01 Published:2013-09-01

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Abstract:

Objective To elucidate the underlying role of T-bet, GATA-3, and Foxp3 in the immune etiology of human with recurrent aphthous ulcer(RAU). Methods Peripheral blood mononuclear cells(PBMCs) were isolated by density gradient centrifugation from 33 cases of minor RAU and 30 cases of healthy controls. The expression levels of T-bet, GATA-3, and Foxp3 mRNA in PBMCs were investigated by reverse transcription-polymerase chain reaction. The cellular and humoral immune status of patients with RAU was investigated by flow cytometry and enzyme-linked immunosorbent assay. Results CD4, CD4/CD8, T+B+NK cells were significantly lower in the RAU group than in the control group(P<0.01). Immunoglobulin(Ig) A was lower in the RAU group than in the control group(P<0.05). IgM and 50% hemolytic complement were significantly higher in the RAU group than in the control group(P<0.05). The expression level of T-bet mRNA and Foxp3 mRNA, the ratio of T-bet/GATA-3 in the RAU group were lower than that in the control group(P<0.01). Meanwhile, the expression level of GATA-3 mRNA in the RAU group was higher than that in the control group(P<0.05). A positive correlation was detected between Foxp3 mRNA level and T-bet/GATA-3 ratio(r=0.404, P=0.02). Conclusion Most RAU patients have immune cells reduced and humoral immune disorder. The increased expression of GATA-3 and decreased expression of T-bet might be one of the mechanisms of T helper cell(Th)1/Th2 imbalance in RAU, accounting for the abnormal immune function in patients with RAU. The decrease in regulatory T cells might be a contributing factor toward Th1/Th2 imbalance in RAU.

Key words: recurrent aphthous ulcer, T-bet, GATA-3, Foxp3


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