Int J Stomatol

Inter J Stomatol ›› 2011, Vol. 38 ›› Issue (1): 14-18.doi: 10.3969/j.issn.1673-5749.2011.01.004

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Platelet-rich plasma generated via the three centrifugalization method and its impact on adipose tis

XU  Yan, YE  Mei, CHEN  Fang, JIANG  Da-Chuan, SUN  Xiao-Yu, LIN  Xiao, SHEN  Ji-Long   

  1. 1. Dept. of Periodontics, College of Stomatology, Anhui Medical University, Hefei 230032, China; 2. Dept. of Health Service Administration, College of Health Service Administration, Anhui Medical University, Hefei 230032, China; 3. The Key Laboratory of Zoonoses, Anhui Province, Anhui Medical University, Hefei 230032, China
  • Received:2009-12-22 Revised:2010-11-26 Online:2011-01-20 Published:2011-01-20

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Abstract:

Objective  The study investigated an effective method of generating platelet-rich plasma（PRP） and evaluated the impact of different concentrations of PRP on adipose tissue-derived stem cell（ADSC） proliferation and mineralization in vitro. Methods  40 mL whole blood was collected from 3 healthy Beagle dogs and then PRP was separated by the two centrifugalization method and the three centrifugalization method respectively. Then the platelet was counted and analyzed. ADSC were exposed to DMEM medium with various concentrations of PRP （5%, 10%, 15%, 20%） respectively. After incubation of 48, 72, 96 h, the proliferations of ADSC were measured. ADSC were cultured in osteoblasts induced medium and osteoblasts induced medium with PRP（5%, 10%, 15%, 20%） respectively, The osteogenetic expression of ADSC and the influence on its osteogenesis mineralization of PRP were observed. Results   The number of platelet from the three centrifugalization method was more than that from the two centrifugalization method. The role on various concentrations of PRP on cell proliferation was more significant than that of negative control（P<0.001）, the differences between various concentrations of PRP groups had a statistical significance（P<0.001）. The group of 96 h, 15% PRP reached to pe ak value in ADSC proliferation. The quantity of mineralization node in ADSC was maximized under the condition of osteoblasts induced medium with 20% PRP. Conclusion   The three centrifugalization method was more efficient than the two centrifugalization method. PRP promoted ADSC proliferation in both the dose- and time- dependent ways while PRP improved ADSC mineralization in the dose-dependent way only.

Key words: platelet-rich plasma, adipose tissue-derived stem cell, cell proliferation, mineralization

CLC Number: 

  • Q 813.1+

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