国际口腔医学杂志

国际口腔医学杂志 ›› 2026, Vol. 53 ›› Issue (6): 833-841.doi: 10.7518/gjkq.2026141

• 论著 • 上一篇    

circFNDC3B调控微小RNA-206/膜联蛋白A2通路对口腔鳞状细胞癌SCC-9细胞生物学行为的影响

赵晨蕾1,2(),郭杰2,靳琳玉2,李松3,庄志征3,仇永乐1,2()   

  1. 1.河北医科大学口腔医学院 石家庄 050000
    2.河北医科大学第四医院口腔科 石家庄 050000
    3.河北大学附属医院口腔科 保定 071000
  • 收稿日期:2026-02-02 修回日期:2026-09-03 出版日期:2026-11-01 发布日期:2026-10-29
  • 通讯作者: 仇永乐
  • 作者简介:赵晨蕾， 医师， 硕士，Email：chenlei_zhao11@163.com
  • 基金资助:
    河北省自然科学基金(H2024206476);河北省医学科学研究课题(20250693)

Effect of circFNDC3B regulation of the miRNA-206/annexin A2 pathway on the biological behavior of oral squamous cell carcinoma SCC-9 cells

Chenlei Zhao1,2(),Jie Guo2,Linyu Jin2,Song Li3,Zhizheng Zhuang3,Yongle Qiu1,2()   

  1. 1.School of Stomatology, Hebei Medical University, Shijiazhuang 050000, China
    2.Dept. of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China
    3.Dept. of Stomatology, Affiliated Hospital of Hebei University, Baoding 071000, China
  • Received:2026-02-02 Revised:2026-09-03 Online:2026-11-01 Published:2026-10-29
  • Contact: Yongle Qiu
  • Supported by:
    Hebei Natural Science Foundation(H2024206476);Medical Science Research Project of Hebei(20250693)

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摘要：

目的 探讨circFNDC3B对口腔鳞状细胞癌（OSCC）细胞恶性生物学行为的作用及其与miR-206和膜联蛋白A2（ANXA2）之间的调控关系。 方法 实时定量聚合酶链反应（qRT-PCR）和Western blot分别检测OSCC组织、癌旁组织、人口腔鳞状细胞癌细胞系SCC-9细胞和人口腔角质形成细胞（HOK）中circFNDC3B、miR-206和ANXA2的mRNA及相关蛋白表达；采用噻唑蓝（MTT）、划痕愈合、Transwell实验及流式细胞术分析circFNDC3B沉默后对SCC-9细胞增殖活力、迁移及侵袭能力、细胞周期及凋亡的影响；双萤光素酶报告基因检测验证circFNDC3B、miR-206和ANXA2的基因靶向作用关系。分析circFNDC3B表达同患者临床病理特征和治疗预后的关联性。 结果 circFNDC3B在OSCC组织和细胞中高表达。沉默circFNDC3B通过上调miR-206进而抑制ANXA2表达，导致SCC-9细胞的增殖、迁移及侵袭能力降低，G0/G1期的细胞比例和凋亡率增加。circFNDC3B的表达仅与患者淋巴结转移相关，circFNDC3B高表达的患者总生存率较低。 结论 circFNDC3B靶向调控miR-206/ANXA2信号通路，促进OSCC细胞的恶性生物学行为。

关键词: 口腔鳞状细胞癌, circFNDC3B, 微小RNA-206, 膜联蛋白A2, 增殖, 侵袭

Abstract:

Objective This work aimed to explore the utility of circFNDC3B in malignant biological behavior of oral squamous cell carcinoma (OSCC) and its regulatory relationship with miRNA (miR)-206 and annexin A2 (ANXA2). Methods Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect the mRNA and related protein expression levels of circFNDC3B, miR-206, and ANXA2 in OSCC tissue, cancerous tissue, SCC-9 cells, and human oral keratinocytes (HOK) cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT), scratch healing, Transwell, and flow cytometry were used to investigate the effects of circFNDC3B silencing on the proliferation, migration, invasion, cycle, and apoptosis of SCC-9 cells. Dual luciferase assay was applied to validate the gene targeting relationship of circFNDC3B, miR-206, and ANXA2. The association between circFNDC3B expression and patients’ clinical pathological characteristics and treatment prognosis was analyzed. Results In OSCC tissue cells, circFNDC3B showed high expression. Silencing circFNDC3B inhibited ANXA2 expression by upregulating miR-206, leading to decreased proliferation, migration, and invasion capabilities of SCC-9 cells; increased proportions of cells in the G0/G1 phase; and increased apoptosis rates. Its expression was only linked to lymph node metastasis in patients, and high-expressing patients had lower overall survival. Conclusion CircFNDC3B influen-ces the miR-206/ANXA2 signaling axis, enhancing aggressive traits in OSCC cells.

Key words: oral squamous cell carcinoma, circFNDC3B, miRNA-206, annexin A2, proliferation, invasion

中图分类号: 

  • R739.8

表 1

引物序列"

基因引物
circFNDC3B正向引物：5'-TCAGTGCATTCAAGGAAGCC-3'
反向引物：5'-CCATCCTCTGCTCTTATTGTGAA-3'
miR-206正向引物：5'-AGGCCACATGCTTCTTTATATCCC-3'
反向引物：5'-CGAAACCACACACTTCCTTACATT-3'
ANXA2正向引物：5'-GGAGATGACTGAAGCCCGACA-3'
反向引物：5'-GAAGGCCAGGCAATGCTTAGG-3'
U6正向引物：5'-CGCTTCGGCAGCACATATACTA-3'
反向引物：5'-ATGGAACGCTTCACGAATTTGC-3'
GAPDH正向引物：5'-GCTTCGGCAGCACATATA-3'
反向引物：5'-ATTTGCGTGTCATCCTTGC-3'

图1

qRT-PCR检测各组基因的相对表达量A：circFNDC3B；B：miR-206；C：ANXA2。与癌旁组织相比，*P<0.05；与HOK相比，**P<0.01。"

表 2

沉默circFNDC3B对SCC-9细胞中circFNDC3B、miR-206、ANXA2表达水平的影响"

组别circFNDC3BmiR-206ANXA2
ctrl组0.99±0.051.05±0.101.10±0.20
si-NC组0.98±0.051.05±0.111.19±0.20
si-FNDC3B组0.45±0.07*#1.69±0.10*#0.55±0.15*#
si-FNDC3B+miR-206 NC组0.44±0.051.71±0.120.53±0.15
si-FNDC3B+miR-206 inhibitor组0.47±0.051.25±0.10@&0.89±0.18@&

图2

MTT法检测各组细胞增殖情况与ctrl组、si-NC组相比，*P<0.05；与si-FNDC3B+miR-206 NC组相比，**P<0.01。"

图 3

划痕愈合实验检测各组细胞迁移情况 倒置光学显微镜"

图 4

Transwell实验检测各组细胞侵袭情况 倒置光学显微镜"

图 5

流式细胞术检测各组细胞周期情况及统计图"

图 6

流式细胞术检测各组细胞凋亡情况及统计图与ctrl组和si-NC组相比，*P<0.05；与si-FNDC3B+miR-206 NC组相比，**P<0.01。"

图 7

Western blot检测各组细胞蛋白表达情况及统计图1：ctrl；2：si-NC；3：si-FNDC3B；4：si-FNDC3B+miR-206 NC；5：si-FNDC3B+miR-206 inhibitor。与ctrl组和si-NC组相比，*P<0.05；与si-FNDC3B+miR-206 NC组相比，**P<0.01。"

图 8

circFNDC3B和ANXA2与miR-206靶向结合关系A：数据库预测circFNDC3B、ANXA2与miR-206结合位点；B、C：双萤光素酶报告基因实验检测circFNDC3B（B）和ANXA2（C）与miR-206之间的靶向关系。*P<0.05。"

表 3

circFNDC3B表达水平与OSCC患者临床病理特征的关系 (n（%）)"

临床病理

特征分类

n=75circFNDC3Bχ2P值
高表达（n=45）低表达（n=30）
年龄/岁
<602918（24.00）11（14.67）0.0910.763
≥604627（36.00）19（25.33）
性别
男4227 （36.00）15 （20.00）0.8340.361
女3318 （24.00）15 （20.00）
吸烟
是4528（37.33）17（22.67）0.2310.631
否3017（22.67）13（17.33）
肿瘤位置
舌癌2920 （26.67）9（12.00）1.7370.629
颊癌158 （10.66）7（9.33）
牙龈癌2112 （16.00）9（12.00）
其他105（6.67）5（6.67）
T分期
T13015（20.00）15（20.00）5.9270.115
T22213（17.33）9（12.00）
T31612（16.00）4（5.33）
T475（6.67）2（2.67）
淋巴结转移
是3525（33.33）10（13.33）7.2000.007
否4020（26.67）20（26.67）
远处转移
是1510（13.33）5（6.67）0.5840.445
否6035（46.67）25（33.33）
生存状态
生存4021（28.00）19（25.33）2.0090.156
死亡3524（32.00）11（14.67）

图9

OSCC患者总生存率分析"

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