Inter J Stomatol ›› 2017, Vol. 44 ›› Issue (4): 421-425.doi: 10.7518/gjkq.2017.04.010

• Original Articles • Previous Articles     Next Articles

Effect of nicotine and mecamylamine on growth of periodontal pathogens

Liu Shiyu, Tian Mi, Shi Liran, Pan Weilin, Wang Yiyao, Li Mingyun   

  1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2016-07-22 Revised:2017-04-13 Online:2017-07-01 Published:2017-07-01
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(81400501), the Special Funds of State Key Laboratory of Oral Diseases(SKLOD201525) and Sichuan University Students’ Innovative Training Program(20140298).

Abstract: Objective This work aims to study the effects of nicotine and mecamylamine on periodontal pathogen growth and pathogen-formed biofilms. Methods Doubling dilution was used to determine the minimum inhibition concentration(MIC) and minimum bactericidal concentration(MBC) of nicotine on Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans. The influences of 1/16 MIC, 1/8 MIC, 1/4 MIC, 1/2 MIC, and MIC of nicotine on the biofilms formed by P. gingivalis and A. actinomycetemcomitans were studied by light absorbance determination on a microplate reader. Results The MIC of nicotine against planktonic P. gingivalis was 8 mg·mL-1, which was similar to that of A. actinomycetemcomitans. When the nicotine concentration increased by a degree, the absorbance of the two bacterial biofilms increased. However, the absorbance decreased at further increase in nicotine concentration. The absorbance decreased to a minimum at a nicotine concentration of 8 mg·mL-1, which was also the minimum biofilm inhibition concentration. The group with mecamylamine(nicotine-receptor antagonist) showed higher absorbance than the control group. Conclusion Minimal doses of nicotine can promote the biofilm formation of P. gingivalis and A. actinomycetemcomitans, and continued increase in nicotine concentration inhibits biofilm formation. In the presence of mecamylamine, nicotine increases the biofilms formed by the P. gingivalis and A. actinomycetemcomitans. This effect is attributed to the decrease in nicotine concentration caused by mecamylamine.

Key words: nicotine, mecamylamine, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, biofilm

CLC Number: 

  • R780.2

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