Int J Stomatol

Int J Stomatol ›› 2026, Vol. 53 ›› Issue (6): 833-841.doi: 10.7518/gjkq.2026141

• Original Article • Previous Articles    

Effect of circFNDC3B regulation of the miRNA-206/annexin A2 pathway on the biological behavior of oral squamous cell carcinoma SCC-9 cells

Chenlei Zhao1,2(),Jie Guo2,Linyu Jin2,Song Li3,Zhizheng Zhuang3,Yongle Qiu1,2()   

  1. 1.School of Stomatology, Hebei Medical University, Shijiazhuang 050000, China
    2.Dept. of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China
    3.Dept. of Stomatology, Affiliated Hospital of Hebei University, Baoding 071000, China
  • Received:2026-02-02 Revised:2026-09-03 Online:2026-11-01 Published:2026-10-29
  • Contact: Yongle Qiu E-mail:chenlei_zhao11@163.com;qqqyl@hebmu.edu.cn
  • Supported by:
    Hebei Natural Science Foundation(H2024206476);Medical Science Research Project of Hebei(20250693)

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Abstract:

Objective This work aimed to explore the utility of circFNDC3B in malignant biological behavior of oral squamous cell carcinoma (OSCC) and its regulatory relationship with miRNA (miR)-206 and annexin A2 (ANXA2). Methods Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect the mRNA and related protein expression levels of circFNDC3B, miR-206, and ANXA2 in OSCC tissue, cancerous tissue, SCC-9 cells, and human oral keratinocytes (HOK) cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT), scratch healing, Transwell, and flow cytometry were used to investigate the effects of circFNDC3B silencing on the proliferation, migration, invasion, cycle, and apoptosis of SCC-9 cells. Dual luciferase assay was applied to validate the gene targeting relationship of circFNDC3B, miR-206, and ANXA2. The association between circFNDC3B expression and patients’ clinical pathological characteristics and treatment prognosis was analyzed. Results In OSCC tissue cells, circFNDC3B showed high expression. Silencing circFNDC3B inhibited ANXA2 expression by upregulating miR-206, leading to decreased proliferation, migration, and invasion capabilities of SCC-9 cells; increased proportions of cells in the G0/G1 phase; and increased apoptosis rates. Its expression was only linked to lymph node metastasis in patients, and high-expressing patients had lower overall survival. Conclusion CircFNDC3B influen-ces the miR-206/ANXA2 signaling axis, enhancing aggressive traits in OSCC cells.

Key words: oral squamous cell carcinoma, circFNDC3B, miRNA-206, annexin A2, proliferation, invasion

CLC Number: 

  • R739.8

TrendMD: 

Tab 1

The primer sequences"

基因引物
circFNDC3B正向引物：5'-TCAGTGCATTCAAGGAAGCC-3'
反向引物：5'-CCATCCTCTGCTCTTATTGTGAA-3'
miR-206正向引物：5'-AGGCCACATGCTTCTTTATATCCC-3'
反向引物：5'-CGAAACCACACACTTCCTTACATT-3'
ANXA2正向引物：5'-GGAGATGACTGAAGCCCGACA-3'
反向引物：5'-GAAGGCCAGGCAATGCTTAGG-3'
U6正向引物：5'-CGCTTCGGCAGCACATATACTA-3'
反向引物：5'-ATGGAACGCTTCACGAATTTGC-3'
GAPDH正向引物：5'-GCTTCGGCAGCACATATA-3'
反向引物：5'-ATTTGCGTGTCATCCTTGC-3'

Fig 1

qRT-PCR detection of relative expression levels of genes in each group"

Tab 2

Effect of silencing circFNDC3B on the expres-sion levels of circFNDC3B, miR-206 and ANXA2 in SCC-9 cells"

组别circFNDC3BmiR-206ANXA2
ctrl组0.99±0.051.05±0.101.10±0.20
si-NC组0.98±0.051.05±0.111.19±0.20
si-FNDC3B组0.45±0.07*#1.69±0.10*#0.55±0.15*#
si-FNDC3B+miR-206 NC组0.44±0.051.71±0.120.53±0.15
si-FNDC3B+miR-206 inhibitor组0.47±0.051.25±0.10@&0.89±0.18@&

Fig 2

Proliferation of cells in each group using MTT assay"

Fig 3

Migration of cells in each group using scratch healing assay inverted optical microscope"

Fig 4

Invasion of cells in each group using Transwell assay inverted optical microscope"

Fig 5

Cycle of cells in each group using flow cytometry detection and statistical chart"

Fig 6

Apoptosis of cells in each group using Flow cytometry detection and statistical chart"

Fig 7

Expression of cells in each group using Western blot and statistical chart"

Fig 8

circFNDC3B and ANXA2 binding to miR-206"

Tab 3

Relationship between expression level of circFNDC3B and clinical and pathological characteristics of OSCC patients"

临床病理

特征分类

n=75circFNDC3Bχ2P值
高表达（n=45）低表达（n=30）
年龄/岁
<602918（24.00）11（14.67）0.0910.763
≥604627（36.00）19（25.33）
性别
男4227 （36.00）15 （20.00）0.8340.361
女3318 （24.00）15 （20.00）
吸烟
是4528（37.33）17（22.67）0.2310.631
否3017（22.67）13（17.33）
肿瘤位置
舌癌2920 （26.67）9（12.00）1.7370.629
颊癌158 （10.66）7（9.33）
牙龈癌2112 （16.00）9（12.00）
其他105（6.67）5（6.67）
T分期
T13015（20.00）15（20.00）5.9270.115
T22213（17.33）9（12.00）
T31612（16.00）4（5.33）
T475（6.67）2（2.67）
淋巴结转移
是3525（33.33）10（13.33）7.2000.007
否4020（26.67）20（26.67）
远处转移
是1510（13.33）5（6.67）0.5840.445
否6035（46.67）25（33.33）
生存状态
生存4021（28.00）19（25.33）2.0090.156
死亡3524（32.00）11（14.67）

Fig 9

Analysis of overall survival in OSCC patients"

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