Inter J Stomatol ›› 2018, Vol. 45 ›› Issue (5): 522-526.doi: 10.7518/gjkq.2018.05.005

• Implantology • Previous Articles     Next Articles

Research progress on enamel matrix derivative in dental implantation

Mengmeng Mi,Haibin Xia,Min. Wang()   

  1. Dept. of Implantology, Hospital of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2017-12-25 Revised:2018-06-14 Online:2018-09-01 Published:2018-09-20
  • Contact: Min. Wang
  • Supported by:
    This study was supported by National Natural Science Foundation of China(81500866)


Enamel matrix derivative comes from enamel matrix proteins, which are extracted from young pig tooth germ. The derivative can promote regeneration of periodontal tissues, bone induction, and biomineralization. At present, enamel matrix derivative is mainly used to treat periodontal diseases by promoting regeneration of new cementum, insertion of new functionally oriented periodontal ligament fibers, and formation of new alveolar bone. When used in dental implantation, enamel matrix derivative can promote osseointegration of bone-implant interfaces, repair of bone defects in implants, and healing of soft tissue around implants. As dental implantation is applied in increasingly wider settings, this article offers an overview of the use of enamel matrix derivative in dental implantation.

Key words: enamel matrix derivative, dental implantation, hard tissue, soft tissue

CLC Number: 

  • R783.3

[1] Hammarström L, Heijl L, Gestrelius S , Periodontal regeneration in a buccal dehiscence model in mon-keys after application of enamel matrix proteins[J]. J Clin Periodontol, 1997,24(9 Pt 2):669-677.
doi: 10.1111/cpe.1997.24.issue-9
[2] 吴飞俊, 裴路 . 釉基质蛋白衍生物治疗牙周组织再生进展的研究[J]. 医学研究生学报, 2013,26(6):650-653.
Wu FJ, Pei L . Enamel matrix derivative and perio-dontal tissue regeneration[J]. J Med Postgra, 2013,26(6):650-653.
[3] Bartlett JD, Ganss B, Goldberg M , et al. Protein-protein interactions of the developing enamel matrix[J]. Curr Top Dev Biol, 2006,74:57-115.
doi: 10.1016/S0070-2153(06)74003-0
[4] Dacy JA, Spears R, Hallmon WW , et al. Effects of phosphated titanium and enamel matrix derivatives on osteoblast behavior in vitro[J]. Int J Oral Maxillo-fac Implants, 2007,22(5):701-709.
doi: 10.1016/j.ijom.2007.03.014 pmid: 3215085
[5] Miron RJ, Chandad F, Buser D , et al. Effect of enamel matrix derivative liquid on osteoblast and periodontal ligament cell proliferation and differentiation[J]. J Periodontol, 2016,87(1):91-99.
doi: 10.1902/jop.2015.150389
[6] Miron RJ, Sculean A, Cochran DL , et al. Twenty years of enamel matrix derivative: the past, the pre-sent and the future[J]. J Clin Periodontol, 2016,43(8):668-683.
doi: 10.1111/jcpe.12546 pmid: 26987551
[7] Qu Z, Andrukhov O, Laky M , et al. Effect of enamel matrix derivative on proliferation and differentiation of osteoblast cells grown on the titanium implant surface[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2011,111(4):517-522.
doi: 10.1016/j.tripleo.2010.10.011
[8] Craig RG, Kamer AR, Kallur SP , et al. Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface: a pilot study in the minipig[J]. J Oral Implantol, 2006,32(5):228-236.
doi: 10.1563/820.1
[9] Wada Y, Mizuno M, Nodasaka Y , et al. The effect of enamel matrix derivative on spreading, prolifera-tion, and differentiation of osteoblasts cultured on zirconia[J]. Int J Oral Maxillofac Implants, 2012,27(4):849-858.
[10] 杨旭, 谭建国, 赵辛蓉 , 等. 釉基质蛋白促进骨质疏松症大鼠股骨种植体骨结合的研究[J]. 现代口腔医学杂志, 2006,20(5):505-508.
doi: 10.3969/j.issn.1003-7632.2006.05.020
Yang X, Tan JG, Zhao XR , et al. Effects of enamel matrix proteins on osseointegration of titanium im-plants in osteoporosis Wistar rats femurs[J]. J Modern Somatol, 2006,20(5):505-508.
doi: 10.3969/j.issn.1003-7632.2006.05.020
[11] Birang R, Shahabooei M, Mashhadiabbas F , et al. Effect of enamel matrix derivative on bone formation around intraosseous titanium implant: an experimen-tal study in canine model[J]. Dent Res J (Isfahan), 2012,9(6):790-796.
[12] Wen B, Li Z, Nie R , et al. Influence of biphasic cal-cium phosphate surfaces coated with enamel matrix derivative on vertical bone growth in an extra-oral rabbit model[J]. Clin Oral Implants Res, 2016,27(10):1297-1304.
doi: 10.1111/clr.2016.27.issue-10
[13] Eickholz P, Röllke L, Schacher B , et al. Enamel matrix derivative in propylene glycol alginate for treatment of infrabony defects with or without sys-temic doxycycline: 12- and 24-month results[J]. J Periodontol, 2014,85(5):669-675.
doi: 10.1902/jop.2013.130290
[14] Mrozik KM, Gronthos S, Menicanin D , et al. Effect of coating straumann bone ceramic with emdogain on mesenchymal stromal cell hard tissue forma-tion[J]. Clin Oral Investig, 2012,16(3):867-878.
doi: 10.1007/s00784-011-0558-3
[15] John V, De Poi R, Blanchard S , Socket preservation as a precursor of future implant placement: review of the literature and case reports[J]. Compend Contin Educ Dent, 2007,28(12):646-654, 671.
[16] Alkan EA, Tüter G, Parlar A , et al. Evaluation of peri-implant crevicular fluid prostaglandin E2 levels in augmented extraction sockets by different bioma-terials[J]. Acta Odontol Scand, 2016,74(7):532-538.
doi: 10.1080/00016357.2016.1214979
[17] Favato MN, Vidigal BC, Cosso MG , et al. Impact of human maxillary sinus volume on grafts dimensional changes used in maxillary sinus augmentation: a mul-tislice tomographic study[J]. Clin Oral Implants Res, 2015,26(12):1450-1455.
doi: 10.1111/clr.12488
[18] Simion M, Ferrantino L, Idotta E , et al. The associa-tion of guided bone regeneration and enamel matrix derivative for suprabony reconstruction in the es-thetic area: a case report[J]. Int J Periodontics Resto-rative Dent, 2015,35(6):767-772.
doi: 10.11607/prd.2297
[19] El Helow K , El Askary Ael S. Regenerative barriers in immediate implant placement: a literature review[J]. Implant Dent, 2008,17(3):360-371.
doi: 10.1097/ID.0b013e3181813406 pmid: 18784536
[20] Cangini F, Cornelini R , A comparison between enamel matrix derivative and a bioabsorbable membrane to enhance healing around transmucosal immediate post-extraction implants[J]. J Periodontol, 2005,76(10):1785-1792.
doi: 10.1902/jop.2005.76.10.1785
[21] Lim HC, Lee JS, Jung UW , et al. Bone regenerative potential of enamel matrix protein in the circumfe-rential defect around a dental implant[J]. Implant Dent, 2016,25(2):179-185.
doi: 10.1097/ID.0000000000000383
[22] Franke Stenport V, Johansson CB , Enamel matrix derivative and titanium implants[J]. J Clin Periodon-tol, 2003,30(4):359-363.
doi: 10.1034/j.1600-051X.2003.00326.x
[23] Murai M, Sato S, Koshi R , et al. Effects of the enamel matrix derivative and beta-tricalcium phosphate on bone augmentation within a titanium cap in rabbit calvarium[J]. J Oral Sci, 2005,47(4):209-217.
doi: 10.2334/josnusd.47.209
[24] 黎卫星, 姜玲, 张晓燕 , 等. 种植体周围软组织状态对种植义齿修复的影响[J]. 医学研究生学报, 2011,24(4):420-422.
Li WX, Jiang L, Zhang XY , et al. Influence of the peri-implant soft tissue state to the implant supported denture restoration[J]. J Med Postgra, 2011,24(4):420-422.
[25] Bates C, Marino V, Fazzalari NL , et al. Soft tissue attachment to titanium implants coated with growth factors[J]. Clin Implant Dent Relat Res, 2013,15(1):53-63.
doi: 10.1111/j.1708-8208.2010.00327.x pmid: 21435158
[26] Wang Y, Zhang Y, Jing D , et al. Enamel matrix de-rivative improves gingival fibroblast cell behavior cultured on titanium surfaces[J]. Clin Oral Investig, 2016,20(4):685-695.
doi: 10.1007/s00784-015-1558-5
[27] Guimarães GF, de Araújo VC, Nery JC , et al. Mi-crovessel density evaluation of the effect of enamel matrix derivative on soft tissue after implant place-ment: a preliminary study[J]. Int J Periodontics Restorative Dent, 2015,35(5):733-738.
[28] Schwarz F, Rothamel D, Herten M , et al. Effect of enamel matrix protein derivative on the attachment, proliferation, and viability of human SaOs2 osteo-blasts on titanium implants[J]. Clin Oral Investig, 2004,8(3):165-171.
[29] Lang NP, Berglundh T , Working Group 4 of Seventh European Workshop on Periodontology. Periimplant diseases: where are we now? Consensus of the seventh european workshop on periodontology[J]. J Clin Periodontol, 2011,38(Suppl 11):178-181.
doi: 10.1111/jcpe.2011.38.issue-s11
[30] Lindhe J, Meyle J , Group D of European Workshop on Periodontology. Peri-implant diseases: consensus report of the sixth european workshop on perio-dontology[J]. J Clin Periodontol, 2008,35(8 Suppl):282-285.
doi: 10.1111/cpe.2008.35.issue-s8
[31] Kashefimehr A, Pourabbas R, Faramarzi M , et al. Effects of enamel matrix derivative on non-surgical management of peri-implant mucositis: a double-blind randomized clinical trial[J]. Clin Oral Investig, 2017,21(7):2379-2388.
doi: 10.1007/s00784-016-2033-7
[32] Faramarzi M, Goharfar Z, Pourabbas R , et al. Micro-biological and clinical effects of enamel matrix de-rivative and sustained-release micro-spherical mino-cycline application as an adjunct to non-surgical therapy in peri-implant mucosal inflammation[J]. J Korean Assoc Oral Maxillofac Surg, 2015,41(4):181-189.
doi: 10.5125/jkaoms.2015.41.4.181
[33] Froum SJ, Froum SH, Rosen PS , Successful manage-ment of peri-implantitis with a regenerative approach: a consecutive series of 51 treated implants with 3- to 7.5-year follow-up[J]. Int J Periodontics Restorative Dent, 2012,32(1):11-20.
[34] Isehed C, Holmlund A, Renvert S , et al. Effectiveness of enamel matrix derivative on the clinical and micro-biological outcomes following surgical regenerative treatment of peri-implantitis. A randomized con-trolled trial[J]. J Clin Periodontol, 2016,43(10):863-873.
doi: 10.1111/jcpe.2016.43.issue-10
[35] Froum SJ, Froum SH, Rosen PS , A regenerative approach to the successful treatment of peri-im-plantitis: a consecutive series of 170 implants in 100 patients with 2- to 10-year follow-up[J]. Int J Perio-dontics Restorative Dent, 2015,35(6):857-863.
doi: 10.11607/prd.2571
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