Int J Stomatol

Inter J Stomatol ›› 2018, Vol. 45 ›› Issue (1): 26-31.doi: 10.7518/gjkq.2018.01.005

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The effects and underlying mechanism of lysophosphatidic acid in β-catenin nuclear translocation of dental pulp cells

Fang Hongzhi1, Yang Hui2, Shao Meiying3, Hu Tao4   

  1. 1. Dept. of Stomatology, The Third People’s Hospital of Chengdu, Chengdu 610031, China
    2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of General Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    3. Dept. of Stomatology, No. 4 West China Teaching Hospital, Sichuan University, Chengdu 610041, China
    4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2017-05-15 Revised:2017-10-19 Online:2018-01-01 Published:2018-01-01
  • Supported by:

    This study was supported by National Natural Science Foundation of China (81400504), Applied Basic Research Project of Sichuan Provincial Science and Technology Department (2014JY0073, 2013JY0164) and China Postdoctoral Science Foundation (2014M562332).

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Abstract:

Objective The aim of our study is to investigate the biological effects of lysophosphatidic acid (LPA) on β-catenin accumulation, activation, and nuclear translocation of cultured human dental pulp cells (DPCs) ex vivo. Methods LPA was used to stimulate dental pulp cells, and Y-27632 was used to inhibit Rho-associated protein kinase (ROCK). Fluorescence microscopy and Western blot were used to detect the effects of Rho/ROCK on β-catenin accumulation, activation, and nuclear transloca- tion. Results β-catenin accumulated around the nuclear membrane after LPA stimulation of DPC for 3 h. When treated for 6 or 10 h with LPA, β-catenin translocated into the nucleus in some dental pulp cells; however, nuclear translocation of β-catenin was abrogated by Y-27632. Results of Western blot showed that LPA promoted β-catenin expression and activation. ROCK inhibition also degraded the level of β-catenin activation. Conclusion LPA regulated β-catenin accumulation, activation, and nuclear translocation via the Rho/ROCK signaling pathway.

Key words: dental pulp cell, lysophosphatidic acid, Rho-associated protein kinase, β-catenin

CLC Number: 

  • R37

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