Int J Stomatol

Inter J Stomatol ›› 2012, Vol. 39 ›› Issue (1): 33-36.doi: 10.3969/j.issn.1673-5749.2012.01.009

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Biological effects of nano -hydroxyapatite -aliphatic polyester -amide composite on the osteoblasts

Deng Xia1, Xia Xi2.   

  1. 1. Dept. of Stomatology, Nuclear of Industry 416 Hospital, Chengdu 610051, China; 2. Dept. of Prosthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China
  • Received:2011-04-20 Revised:2011-10-09 Online:2012-01-01 Published:2012-01-01

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Abstract:

Objective To evaluate the biological effects of nano-hydroxyapatite-aliphatic polyester-amide composite（ nHA-PEA） on the osteoblast. Methods The Dulbecco minimum essential medium（DMEM） leaching liquor of nHA-PEA was applied to the osteoblasts of the test groups while the DMEM itself was applied to control. The methyl thiazolyl tetrazolium assay, flow cytometry and alkaline phosphatase（AKP） analysis were used to evaluate the changes in cell growth, cell cycle and cell function. Moreover, osteoblasts were cultured on the surface of nHA-PEA composite and the attachment, growth and proliferation of osteoblast were investigated. Results The cultured osteoblasts grew well and showed nomorphological variation. Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 92%～107% without dose-dependent effect（P>0.05）. The cell cycle and AKP activity were similar in test and control groups（P>0.05）. Good cell attachment and proliferation manner were observed on the membranes. Conclusion nHA-PEA has no negative effects on the osteoblast and its osteoblastcompatibility is proved.

Key words: nano-hydroxyapatite-aliphatic polyester-amide composite, osteoblast, biocompatibility


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