Int J Stomatol

Inter J Stomatol ›› 2017, Vol. 44 ›› Issue (6): 679-685.doi: 10.7518/gjkq.2017.06.011

• Original Articles • Previous Articles     Next Articles

Mechanical strain induces mouse bone mesenchymal stem cells osteogenic differentiation

Xue Lingfa1, Zhang Daizun2, Xiao Wenlin1, Yu Baojun1   

  1. 1. Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University; The Oral Key Laboratory of Education Department of Shandong Province, Qingdao 266555, China;
    2. Dept. of Pediatric Dentistry, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
  • Received:2016-12-23 Revised:2017-07-20 Online:2017-11-01 Published:2017-11-01
  • Supported by:
    ; This study supported by Science and Technology Program in Colleges and Universities of Shandong(J12LK57) and Special Fund for Applied Study and Public Health, Huangdao District, Qingdao(2014-1-84).

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Abstract: Objective This study aimed to investigate the stimulating mechanism of p38 mitogen-activated protein kinase(MAPK) signaling pathway and Osterix under intermittent mechanical stretch tension to accelerate the osteogenic differentiation of bone mesenchymal stem cells(BMSCs) in mice. Methods Three groups of C57BL/6J BMSC, namely, blank control group, strain group, and inhibitor group(p38MAPK signal pathway inhibitor SB203580 + strain), were prepared and exposed to 0.8% intermittent mechanical strain at 0.5 Hz twice a day for 30 min at each time by using a Flexercell strain unit. The cells in the three groups were then harvested on days 1, 3, and 5, respectively. Changes in the mRNA expression of ALP, COLⅠ, and OCN were detected through real time-polymerase chain reaction(RT-PCR) and the protein expression of P-p38MAPK was observed through Western blot analysis. osterix gene was knocked down by small interfering RNA(siRNA), and Osterix protein expression was determined through Western blot. The mRNA expression levels of ALP, COL I, and OCN were identified through RT-PCR. Results Mechanical tension force could promote the mRNA expression of ALP, COL I, OCN, and osterix. osterix silencing could decrease the mRNA expression of ALP, COL I, and OCN. Western blot revealed that the protein expression levels of Osterix and P-p38MAPK in the strain group were significantly higher than those in the control group(P<0.05). The mRNA expression of ALP, COL I, OCN, and osterix decreased after SB203580 was used. Conclusion Intermittent mechanical strain promotes the osteogenic differentiation of BMSC via the p38MAPK-Osterix pathways.

Key words: intermittent stretching force, bone marrow stem cell, osteoblastic differentiation, p38MAPK signal pathway

CLC Number: 

  • Q786

TrendMD: 
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