国际口腔医学杂志

国际口腔医学杂志 ›› 2024, Vol. 51 ›› Issue (1): 45-51.doi: 10.7518/gjkq.2024016

• 论著 • 上一篇    下一篇

Toll样受体和白细胞介素-17单核苷酸多态性与口腔扁平苔藓易感性的相关性研究

李然1,2(),胡月3,张宁4,高瑞芳1,2,王翔宇1,2,葛学军2()   

  1. 1.山西医科大学口腔医（学）院儿童口腔预防科 太原 030001
    2.山西医科大学口腔医（学）院 口腔疾病防治与新材料山西省重点实验室 太原 030001
    3.太原恒伦悦伦口腔医院有限公司儿童口腔科 太原 030006
    4.北京应急总医院口腔科 北京 100028
  • 收稿日期:2023-04-07 修回日期:2023-10-09 出版日期:2024-01-01 发布日期:2024-01-10
  • 通讯作者: 葛学军
  • 作者简介:李然，副教授，博士，Email：lraner@163.com
  • 基金资助:
    山西省基础研究计划项目(202103021223235)

The correlation study between single nucleotide polymorphisms of Toll-like receptor and interleukin-17 and oral lichen planus

Li Ran1,2(),Hu Yue3,Zhang Ning4,Gao Ruifang1,2,Wang Xiangyu1,2,Ge Xuejun2()   

  1. 1.Dept. of Pediatric and Preventive Dentistry, Shanxi Medical University School & Hospital of Stomatology, Taiyuan 030001, China
    2.Shanxi Medical University School & Hospital of Stomatology, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, China
    3.Dept. of Pediatric Dentistry, Homony Long Stomatological Hospital, Taiyuan 030006, China
    4.Dept. of Stomatology, Emergency General Hospital, Beijing 100028, China
  • Received:2023-04-07 Revised:2023-10-09 Online:2024-01-01 Published:2024-01-10
  • Contact: Xuejun Ge
  • Supported by:
    Shanxi Province Basic Research Program(202103021223235)

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摘要：

目的 探讨Toll样受体（TLR）和白细胞介素-17（IL-17）基因的遗传多态性及与口腔扁平苔藓（OLP）的相关性。 方法 选取病例组（OLP组）患者与对照组（未患OLP人群）外周血提取的DNA为研究对象，选取TLR与IL-17上的7个单核苷酸多态性（SNP）位点，分别是TLR3中的rs5743312、TLR2中的rs121917864和IL-17A中的5个SNP位点（rs4711998、rs3819024、rs8193036、rs3748067和rs17878530）。 采用高分辨率熔解曲线分析法进行外周血细胞PCR产物基因多态性检测，并与DNA测序结果比对。统计分析SNP位点与OLP发生的相关性。 结果 rs5743312（TLR3）、rs3819024（IL-17A）与OLP的发生相关，并有统计学意义（P<0.05）。 其余5个SNP位点未检测出与OLP相关。 结论 rs5743312（TLR3）和rs3819024（IL-17A）可能与OLP的易感性相关。

关键词: 单核苷酸多态性, 高分辨率熔解曲线分析, 口腔扁平苔藓, 易感性

Abstract:

Objective This study aimed to explore the genetic polymorphisms of the Toll-like receptor (TLR) and interleukin (IL)-17 genes and their correlation with oral lichen planus (OLP). Methods DNA extracted from the peripheral blood of patients in the case (OLP group) and control (non-OLP population) groups were selected as the research subjects. Seven single nucleotide polymorphisms were attempted in our study, namely, rs5743312 in TLR3, rs121917864 in TLR2, and five others (rs4711998, rs3819024, rs8193036, rs3748067, and rs17878530) in IL-17A. High resolution melting curve analysis was used to detect the gene polymorphism of PCR products in peripheral blood cells and compared with DNA sequencing. The correlation between polymorphic sites and the occurrence of OLP was statistically analyzed. Results We found that rs5743312 (TLR3) and rs3819024 (IL-17A) had a statistically significant association with OLP (P<0.05). The remaining five SNPs cannot be tested for association with OLP. Conclusion rs5743312 (TLR3) and rs-3819024 (IL-17A) may contribute to the initiation of OLP.

Key words: single nucleotide polymorphism, high resolution curve melting analysis, oral lichen planus, susceptibility

中图分类号: 

  • R781.5

表 1

病例组与对照组基线特征"

纳入临床指标病例组（n=83）对照组（n=87）P值
男/女19/6422/650.715 0
平均年龄/岁47.8846.910.630 7
年龄范围/岁17~7821~60
病程/月1.0~36.0
病程均数/月11.3
糜烂型/n55
非糜烂型/n28

表 2

rs5743312、rs4711998和rs3819024的HWE检验"

检验参数rs5743312rs4711998rs3819024
基因型CT/TTAG/GGAG/GG
n27/1334/1643/5
χ2值5.261.743.05
P值0.072 20.418 70.217 1

图 1

PCR-HRM对SNP位点熔解曲线图A：rs5743312；B：rs4711998；C：rs3819024。"

图 2

PCR-HRM对SNP位点分型曲线图A：rs5743312；B：rs4711998；C：rs3819024。"

图 3

DNA测序结果A：rs5743312；B：rs4711998；C：rs3819024。"

表 3

OLP与rs5743312、rs4711998和rs3819024等位基因频率分布及多态性关联分析"

SNP组别例数/n等位基因型（n/%）χ2值P值OR95% CI
C/GT/A
rs5743312病例组83113/68.053/32.06.8460.0091.9311.175, 3.174
对照组87140/80.034/20.0
rs4711998病例组8366/39.8100/60.20.8020.3701.2230.787, 1.094
对照组8761/35.1113/64.9
rs3819024病例组8353/31.9113/68.112.91<0.0010.4480.288, 0.697
对照组8789/51.185/48.9

表 4

OLP与rs5743312、rs4711998和rs3819024位点多态性关联分析"

SNP组别例数/n基因型（n/%）χ2值P值OR95% CI
次要等位纯合型至少含1个主要等位基因性型
rs5743312病例组8313/15.770/84.34.4110.0363.0461.035, 8.965
对照组875/10.682/89.4
rs4711998病例组8316/19.367/80.81.3990.2371.650.716, 3.803
对照组8711/12.676/87.4
rs3819024病例组835/6.078/94.015.076<0.0016.290.058, 0.439
对照组8725/28.762/71.3
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