钛合金表面氧化石墨烯涂层的制备

doi:10.7518/gjkq.2018.05.008

国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (5): 539-545.doi: 10.7518/gjkq.2018.05.008

钛合金表面氧化石墨烯涂层的制备

邓雪阳,潘兰兰,胡婷,李文华,向学熔()

重庆医科大学附属口腔医院牙周科口腔疾病与生物医学重庆市重点实验室重庆市高校市级口腔生物医学工程重点实验室重庆 401147

收稿日期:2017-10-19 修回日期:2018-04-10 出版日期:2018-09-01 发布日期:2018-09-20
通讯作者: 向学熔
作者简介:邓雪阳,硕士,Email：gushuzi188@sina.com
基金资助:
重庆医科大学国家自然科学基金预研项目(NSFYY20151- 6);重庆市渝北区科技计划项目(2015 Social 10);重庆市高校创新团队建设计划资助项目(CXTDG201602006)

Preparation of graphene oxide coatings on titanium alloy surface

Xueyang Deng,Lanlan Pan,Ting Hu,Wenhua Li,Xuerong. Xiang()

Dept. of Periodontology, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China

Received:2017-10-19 Revised:2018-04-10 Online:2018-09-01 Published:2018-09-20
Contact: Xuerong. Xiang
Supported by:
This study was supported by Pre-Research Project of National Natural Science Foundation of China in Chongqing Medical University(NSFYY20151- 6);Science and Technology Planning Project in Yubei District of Chongqing(2015 Social 10);Project Supported by Program for Innovation Team Building at Institutions of Higher Education in Chongqing(CXTDG201602006)

摘要/Abstract

摘要：

目的利用氧化石墨烯（GO）制备钛合金表面涂层,初步评价其生物相容性。方法采用层层自组装的方法,在碱热处理后的钛合金表面形成由GO阳离子（GO-NH₃ ⁺）、GO阴离子（GO-COO ^-）溶液组成的氧化石墨烯涂层。使用接触角测量仪、扫描电子显微镜、原子力显微镜、万能材料试验机初步分析其亲水性、表面形貌、涂层厚度、涂层粘接强度等特征,扫描电子显微镜、荧光显微镜观察骨髓间充质干细胞在其表面的生长情况,贴膜实验观察其抑菌性能。结果通过层层自组装方法在碱热处理后的钛合金表面制备出具有较好亲水性和粘接强度的、纳米级形貌的GO涂层。骨髓间充质干细胞在具有GO涂层的钛片的表面易于黏附,而且骨髓间充质干细胞活性保持良好。GO涂层对牙龈卟啉单胞菌的杀菌率为86.34%。结论 GO涂层的钛合金有良好的亲水性、生物相容性及抗菌性。

关键词: 氧化石墨烯, 表面涂层, 生物相容性, 抑菌

Abstract:

Objective To prepare a kind of coating on the titanium alloy surface with graphene oxide (GO) and to evaluate its biocompatibility preliminarily. Methods Ti substrates prepared by the alkali-hydrothermal reaction were coated with GO (Ti/GO) through layer-by-layer assembly of positively (GO-NH₃ ⁺) and negatively (GO-COO ^-) charged GO sheet with fifteen bilayers. The hydrophobic nature of GO coating on Ti substrates was analyzed by contact angle meter, and the microstructure of GO coating on Ti substrates were characterized by scanning electron microscope (SEM) and atomic force microscope (AFM). The in vitro cellular response was observed by the biological behaviors of bone marrow mesenchymal stem cells (BMSCs) which were inoculated on Ti substrates by SEM and fluorescence microscopy. Porphyromonas gingivalis was utilized to test the antibacterial effect of the Ti/GO surface. Results The GO coating with nano-scale surface topography on the aikali-hydrothermal reacted Ti alloy discs which was hydrophilic was prepared. The GO coating improved the adhension of BMSCs and kept cells live. 86.34% of bacteria had been killed on the Ti/GO surface. Conclusion The GO-coated Ti discs had good biocompatibility and antibacterial property.

Key words: graphene oxide, surface coating, biocom-patibility, antibacterial property

中图分类号:

R783.1

邓雪阳,潘兰兰,胡婷,李文华,向学熔. 钛合金表面氧化石墨烯涂层的制备[J]. 国际口腔医学杂志, 2018, 45(5): 539-545.

Xueyang Deng,Lanlan Pan,Ting Hu,Wenhua Li,Xuerong. Xiang. Preparation of graphene oxide coatings on titanium alloy surface[J]. Inter J Stomatol, 2018, 45(5): 539-545.

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钛合金表面氧化石墨烯涂层的制备

Preparation of graphene oxide coatings on titanium alloy surface

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