Inter J Stomatol ›› 2013, Vol. 40 ›› Issue (3): 288-290.doi: 10.7518/gjkq.2013.03.003
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Wang Xiaojing, Zhu Zhuoli, Yu Haiyang
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Objective To evaluate the proliferation and differentiation of human umbilical vein endothelial cell(HUVEC) under microvibration. Methods HUVECs were randomly divided into three groups: Group A was blank control group, group B was vibrated under 80 Hz, 30 min per day, group C was vibrated under 40 Hz, 30 min per day; observed HUVEC proliferations based on micro-vibration duration on 0, 3, 5, 7 d. Methyl thiazolyl tetrazolium(MTT) assay was used to detect cell proliferation and growth curve. According to the result of the growth curve, we took another HUVECs divided into 4 groups:Group a was vibrated immediately, group b was blank control of group a, group c was vibrated during three days, group d was blank control of group c. Western blot assay was used to detect secretion of intercellular adhesion molecule(ICAM)-1, endothelial nitric oxide synthase(eNOS) and phosphorylate-eNOS. Results MTT growth curve showed the proliferation mainly from the first to the third day, stepped into the plateau period from the fifth day. Group C proliferated more quickly than in group B from the first day, more quickly than group A from the third day, proliferation capacity of group B was always lower than group A, and the differences were statistically significant(P<0.05). The expressions of ICAM-1 and PeNOS in group c were the highest in all groups. Conclusion The low amplitude high frequency microvibration promoted ICAM-1 and P-eNOS expression, enhanced cell adhesion.
Key words: microvibration, human umbilical vein endothelial cell, proliferation
Wang Xiaojing, Zhu Zhuoli, Yu Haiyang. Proliferation and differentiation of the vascular endothelial cell under microvibration[J].Int J Stomatol, 2013, 40(3): 288-290.
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URL: http://www.gjkqyxzz.cn/EN/10.7518/gjkq.2013.03.003
http://www.gjkqyxzz.cn/EN/Y2013/V40/I3/288
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