Inter J Stomatol ›› 2013, Vol. 40 ›› Issue (3): 288-290.doi: 10.7518/gjkq.2013.03.003

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Proliferation and differentiation of the vascular endothelial cell under microvibration

Wang Xiaojing, Zhu Zhuoli, Yu Haiyang   

  1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University,Chengdu 610041, China
  • Received:2013-01-15 Revised:2013-02-28 Online:2013-05-01 Published:2013-05-01

Abstract:

Objective To evaluate the proliferation and differentiation of human umbilical vein endothelial cell(HUVEC) under microvibration. Methods HUVECs were randomly divided into three groups: Group A was blank control group, group B was vibrated under 80 Hz, 30 min per day, group C was vibrated under 40 Hz, 30 min per day; observed HUVEC proliferations based on micro-vibration duration on 0, 3, 5, 7 d. Methyl thiazolyl tetrazolium(MTT) assay was used to detect cell proliferation and growth curve. According to the result of the growth curve, we took another HUVECs divided into 4 groups:Group a was vibrated immediately, group b was blank control of group a, group c was vibrated during three days, group d was blank control of group c. Western blot assay was used to detect secretion of intercellular adhesion molecule(ICAM)-1, endothelial nitric oxide synthase(eNOS) and phosphorylate-eNOS. Results MTT growth curve showed the proliferation mainly from the first to the third day, stepped into the plateau period from the fifth day. Group C proliferated more quickly than in group B from the first day, more quickly than group A from the third day, proliferation capacity of group B was always lower than group A, and the differences were statistically significant(P<0.05). The expressions of ICAM-1 and PeNOS in group c were the highest in all groups. Conclusion The low amplitude high frequency microvibration promoted ICAM-1 and P-eNOS expression, enhanced cell adhesion.

Key words: microvibration, human umbilical vein endothelial cell, proliferation


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